Heritable genome editing in C. elegans via a CRISPR-Cas9 system

CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C. elegans germline, utilizing single-guide RNAs that are expressed from a U6 small...

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Autores principales: Friedland, Ari E., Tzur, Yonatan B., Esvelt, Kevin M., Colaiácovo, Monica P., Church, George M., Calarco, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822328/
https://www.ncbi.nlm.nih.gov/pubmed/23817069
http://dx.doi.org/10.1038/nmeth.2532
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author Friedland, Ari E.
Tzur, Yonatan B.
Esvelt, Kevin M.
Colaiácovo, Monica P.
Church, George M.
Calarco, John A.
author_facet Friedland, Ari E.
Tzur, Yonatan B.
Esvelt, Kevin M.
Colaiácovo, Monica P.
Church, George M.
Calarco, John A.
author_sort Friedland, Ari E.
collection PubMed
description CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C. elegans germline, utilizing single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
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spelling pubmed-38223282014-02-01 Heritable genome editing in C. elegans via a CRISPR-Cas9 system Friedland, Ari E. Tzur, Yonatan B. Esvelt, Kevin M. Colaiácovo, Monica P. Church, George M. Calarco, John A. Nat Methods Article CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C. elegans germline, utilizing single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants. 2013-06-30 2013-08 /pmc/articles/PMC3822328/ /pubmed/23817069 http://dx.doi.org/10.1038/nmeth.2532 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Friedland, Ari E.
Tzur, Yonatan B.
Esvelt, Kevin M.
Colaiácovo, Monica P.
Church, George M.
Calarco, John A.
Heritable genome editing in C. elegans via a CRISPR-Cas9 system
title Heritable genome editing in C. elegans via a CRISPR-Cas9 system
title_full Heritable genome editing in C. elegans via a CRISPR-Cas9 system
title_fullStr Heritable genome editing in C. elegans via a CRISPR-Cas9 system
title_full_unstemmed Heritable genome editing in C. elegans via a CRISPR-Cas9 system
title_short Heritable genome editing in C. elegans via a CRISPR-Cas9 system
title_sort heritable genome editing in c. elegans via a crispr-cas9 system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822328/
https://www.ncbi.nlm.nih.gov/pubmed/23817069
http://dx.doi.org/10.1038/nmeth.2532
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