Cargando…

FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth

Four and a half LIM protein 1 (FHL1) belongs to the Lin-1, Isl-1 and Mec-3 (LIM)-only protein family and plays important roles in muscle growth and carcinogenesis. However, the biological function of FHL1 remains largely unknown. Here, we show that FHL1 physically and functionally interacted with oe...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Lihua, Niu, Chang, Zheng, Yiqiong, Xiong, Zhihong, Liu, Yufei, Lin, Jing, Sun, Huiwei, Huang, Ke, Yang, Wen, Li, Xiru, Ye, Qinong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822495/
https://www.ncbi.nlm.nih.gov/pubmed/19840196
http://dx.doi.org/10.1111/j.1582-4934.2009.00938.x
_version_ 1782290415501705216
author Ding, Lihua
Niu, Chang
Zheng, Yiqiong
Xiong, Zhihong
Liu, Yufei
Lin, Jing
Sun, Huiwei
Huang, Ke
Yang, Wen
Li, Xiru
Ye, Qinong
author_facet Ding, Lihua
Niu, Chang
Zheng, Yiqiong
Xiong, Zhihong
Liu, Yufei
Lin, Jing
Sun, Huiwei
Huang, Ke
Yang, Wen
Li, Xiru
Ye, Qinong
author_sort Ding, Lihua
collection PubMed
description Four and a half LIM protein 1 (FHL1) belongs to the Lin-1, Isl-1 and Mec-3 (LIM)-only protein family and plays important roles in muscle growth and carcinogenesis. However, the biological function of FHL1 remains largely unknown. Here, we show that FHL1 physically and functionally interacted with oestrogen receptors (ERs), which are involved in breast cancer development and progression. FHL1 bound specifically to the activation function-1 domain of ER. Physical interaction of FHL1 and ER is required for FHL1 repression of oestrogen-responsive gene transcription. FHL1 affected recruitment of ER to an oestrogen-responsive promoter and ER binding to an oestrogen-responsive element. Overexpression of FHL1 in breast cancer cells decreased expression of oestrogen-responsive proteins, whereas knockdown of endogenous FHL1 with FHL1 small interfering RNA increased the expression of these proteins. Further analysis of 46 breast cancer samples showed that FHL1 expression negatively associated with oestrogen-responsive gene expression in breast cancer cells. FHL1 inhibited anchorage-dependent and -independent breast cancer cell growth. These results suggest that FHL1 may play an important role in ER signalling as well as breast cancer cell growth regulation.
format Online
Article
Text
id pubmed-3822495
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-38224952015-04-06 FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth Ding, Lihua Niu, Chang Zheng, Yiqiong Xiong, Zhihong Liu, Yufei Lin, Jing Sun, Huiwei Huang, Ke Yang, Wen Li, Xiru Ye, Qinong J Cell Mol Med Articles Four and a half LIM protein 1 (FHL1) belongs to the Lin-1, Isl-1 and Mec-3 (LIM)-only protein family and plays important roles in muscle growth and carcinogenesis. However, the biological function of FHL1 remains largely unknown. Here, we show that FHL1 physically and functionally interacted with oestrogen receptors (ERs), which are involved in breast cancer development and progression. FHL1 bound specifically to the activation function-1 domain of ER. Physical interaction of FHL1 and ER is required for FHL1 repression of oestrogen-responsive gene transcription. FHL1 affected recruitment of ER to an oestrogen-responsive promoter and ER binding to an oestrogen-responsive element. Overexpression of FHL1 in breast cancer cells decreased expression of oestrogen-responsive proteins, whereas knockdown of endogenous FHL1 with FHL1 small interfering RNA increased the expression of these proteins. Further analysis of 46 breast cancer samples showed that FHL1 expression negatively associated with oestrogen-responsive gene expression in breast cancer cells. FHL1 inhibited anchorage-dependent and -independent breast cancer cell growth. These results suggest that FHL1 may play an important role in ER signalling as well as breast cancer cell growth regulation. Blackwell Publishing Ltd 2011-01 2009-10-16 /pmc/articles/PMC3822495/ /pubmed/19840196 http://dx.doi.org/10.1111/j.1582-4934.2009.00938.x Text en © 2011 The Author Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Articles
Ding, Lihua
Niu, Chang
Zheng, Yiqiong
Xiong, Zhihong
Liu, Yufei
Lin, Jing
Sun, Huiwei
Huang, Ke
Yang, Wen
Li, Xiru
Ye, Qinong
FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth
title FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth
title_full FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth
title_fullStr FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth
title_full_unstemmed FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth
title_short FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth
title_sort fhl1 interacts with oestrogen receptors and regulates breast cancer cell growth
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822495/
https://www.ncbi.nlm.nih.gov/pubmed/19840196
http://dx.doi.org/10.1111/j.1582-4934.2009.00938.x
work_keys_str_mv AT dinglihua fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT niuchang fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT zhengyiqiong fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT xiongzhihong fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT liuyufei fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT linjing fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT sunhuiwei fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT huangke fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT yangwen fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT lixiru fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth
AT yeqinong fhl1interactswithoestrogenreceptorsandregulatesbreastcancercellgrowth