Cargando…

Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice

Cyclooxygenase-2 (COX-2)-dependent prostaglandin (PG) E(2) synthesis in the spinal cord plays a major role in the development of inflammatory hyperalgesia and allodynia. Microsomal PGE(2) synthase-1 (mPGES-1) isomerizes COX-2-derived PGH(2) to PGE(2). Here, we evaluated the effect of mPGES-1-deficie...

Descripción completa

Detalles Bibliográficos
Autores principales: Brenneis, Christian, Coste, Ovidiu, Schmidt, Ronald, Angioni, Carlo, Popp, Laura, Nusing, Rolf M, Becker, Wiebke, Scholich, Klaus, Geisslinger, Gerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822549/
https://www.ncbi.nlm.nih.gov/pubmed/18419601
http://dx.doi.org/10.1111/j.1582-4934.2007.00110.x
_version_ 1782290424757485568
author Brenneis, Christian
Coste, Ovidiu
Schmidt, Ronald
Angioni, Carlo
Popp, Laura
Nusing, Rolf M
Becker, Wiebke
Scholich, Klaus
Geisslinger, Gerd
author_facet Brenneis, Christian
Coste, Ovidiu
Schmidt, Ronald
Angioni, Carlo
Popp, Laura
Nusing, Rolf M
Becker, Wiebke
Scholich, Klaus
Geisslinger, Gerd
author_sort Brenneis, Christian
collection PubMed
description Cyclooxygenase-2 (COX-2)-dependent prostaglandin (PG) E(2) synthesis in the spinal cord plays a major role in the development of inflammatory hyperalgesia and allodynia. Microsomal PGE(2) synthase-1 (mPGES-1) isomerizes COX-2-derived PGH(2) to PGE(2). Here, we evaluated the effect of mPGES-1-deficiency on the noci-ceptive behavior in various models of nociception that depend on PGE(2) synthesis. Surprisingly, in the COX-2-dependent zymosan-evoked hyperalgesia model, the nociceptive behavior was not reduced in mPGES-1-deficient mice despite a marked decrease of the spinal PGE(2) synthesis. Similarly, the nociceptive behavior was unaltered in mPGES-1-deficient mice in the formalin test. Importantly, spinal cords and primary spinal cord cells derived from mPGES-1-deficient mice showed a redirection of the PGE(2) synthesis to PGD(2), PGF(2α) and 6-keto-PGF(1α) (stable metabolite of PGI(2)). Since the latter prostaglandins serve also as mediators of noci-ception they may compensate the loss of PGE(2) synthesis in mPGES-1-deficient mice.
format Online
Article
Text
id pubmed-3822549
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-38225492015-04-27 Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice Brenneis, Christian Coste, Ovidiu Schmidt, Ronald Angioni, Carlo Popp, Laura Nusing, Rolf M Becker, Wiebke Scholich, Klaus Geisslinger, Gerd J Cell Mol Med Images in Cellular / Molecular Medicine Cyclooxygenase-2 (COX-2)-dependent prostaglandin (PG) E(2) synthesis in the spinal cord plays a major role in the development of inflammatory hyperalgesia and allodynia. Microsomal PGE(2) synthase-1 (mPGES-1) isomerizes COX-2-derived PGH(2) to PGE(2). Here, we evaluated the effect of mPGES-1-deficiency on the noci-ceptive behavior in various models of nociception that depend on PGE(2) synthesis. Surprisingly, in the COX-2-dependent zymosan-evoked hyperalgesia model, the nociceptive behavior was not reduced in mPGES-1-deficient mice despite a marked decrease of the spinal PGE(2) synthesis. Similarly, the nociceptive behavior was unaltered in mPGES-1-deficient mice in the formalin test. Importantly, spinal cords and primary spinal cord cells derived from mPGES-1-deficient mice showed a redirection of the PGE(2) synthesis to PGD(2), PGF(2α) and 6-keto-PGF(1α) (stable metabolite of PGI(2)). Since the latter prostaglandins serve also as mediators of noci-ception they may compensate the loss of PGE(2) synthesis in mPGES-1-deficient mice. Blackwell Publishing Ltd 2008-04 2007-08-31 /pmc/articles/PMC3822549/ /pubmed/18419601 http://dx.doi.org/10.1111/j.1582-4934.2007.00110.x Text en ©2008 The Authors Journal compilation © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Images in Cellular / Molecular Medicine
Brenneis, Christian
Coste, Ovidiu
Schmidt, Ronald
Angioni, Carlo
Popp, Laura
Nusing, Rolf M
Becker, Wiebke
Scholich, Klaus
Geisslinger, Gerd
Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice
title Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice
title_full Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice
title_fullStr Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice
title_full_unstemmed Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice
title_short Consequences of altered eicosanoid patterns for nociceptive processing in mPGES-1-deficient mice
title_sort consequences of altered eicosanoid patterns for nociceptive processing in mpges-1-deficient mice
topic Images in Cellular / Molecular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822549/
https://www.ncbi.nlm.nih.gov/pubmed/18419601
http://dx.doi.org/10.1111/j.1582-4934.2007.00110.x
work_keys_str_mv AT brenneischristian consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT costeovidiu consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT schmidtronald consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT angionicarlo consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT popplaura consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT nusingrolfm consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT beckerwiebke consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT scholichklaus consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice
AT geisslingergerd consequencesofalteredeicosanoidpatternsfornociceptiveprocessinginmpges1deficientmice