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Selective CB2 up-regulation in women affected by endometrial inflammation

Endometritis is defined as an inflammation of the endometrial mucosa of the uterus. In endometritis large amounts of toxic mediators, including nitric oxide (NO) are released by inflammatory cells. As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanis...

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Autores principales: Iuvone, Teresa, De Filippis, Daniele, Di Spiezio Sardo, Attilio, D'Amico, Alessandra, Simonetti, Sara, Sparice, Stefania, Esposito, Giuseppe, Bifulco, Giuseppe, Insabato, Luigi, Nappi, Carmine, Guida, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822551/
https://www.ncbi.nlm.nih.gov/pubmed/18419603
http://dx.doi.org/10.1111/j.1582-4934.2007.00085.x
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author Iuvone, Teresa
De Filippis, Daniele
Di Spiezio Sardo, Attilio
D'Amico, Alessandra
Simonetti, Sara
Sparice, Stefania
Esposito, Giuseppe
Bifulco, Giuseppe
Insabato, Luigi
Nappi, Carmine
Guida, Maurizio
author_facet Iuvone, Teresa
De Filippis, Daniele
Di Spiezio Sardo, Attilio
D'Amico, Alessandra
Simonetti, Sara
Sparice, Stefania
Esposito, Giuseppe
Bifulco, Giuseppe
Insabato, Luigi
Nappi, Carmine
Guida, Maurizio
author_sort Iuvone, Teresa
collection PubMed
description Endometritis is defined as an inflammation of the endometrial mucosa of the uterus. In endometritis large amounts of toxic mediators, including nitric oxide (NO) are released by inflammatory cells. As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanisms, by changing the endo-cannabinoid system (ECS) which comprises both CB(1) and CB(2) cannabinoid receptors and their endogenous ligands. The aim of our study was to seek out evidence for the presence of cannabinoid receptors in inflammatory endometrial tissue as well as for their potential role in endometrial inflammation. Our results showed a selective up-regulation of both transcription and expression of CB(2) receptors in biopsies from women affected by endometrial inflammation compared to healthy women. The experiments with the nitric oxide-donor S-Nitroso-L-Glutathione (GSNO) suggest that such a selective up-regulation may be related to the nitric oxide release occurring during endometrial inflammation. In addition, we demonstrated an increase in chymase expression, a marker of mast cells, in biopsies of women affected by endometritis. Therefore our results support the hypothesis that the up-regulation of CB(2) occurs mainly on mast cells and that it might tend to sensitize these cells to the anti-inflammatory effect exerted by endogenous cannabinoids by binding their receptor and thus preventing the mast cell degranulation and the release of pro-inflammatory mediators. In conclusion, we believe that the selective CB(2) up-regulation might play a role as a novel prognostic factor in endometrial inflammation.
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spelling pubmed-38225512015-04-27 Selective CB2 up-regulation in women affected by endometrial inflammation Iuvone, Teresa De Filippis, Daniele Di Spiezio Sardo, Attilio D'Amico, Alessandra Simonetti, Sara Sparice, Stefania Esposito, Giuseppe Bifulco, Giuseppe Insabato, Luigi Nappi, Carmine Guida, Maurizio J Cell Mol Med Images in Cellular / Molecular Medicine Endometritis is defined as an inflammation of the endometrial mucosa of the uterus. In endometritis large amounts of toxic mediators, including nitric oxide (NO) are released by inflammatory cells. As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanisms, by changing the endo-cannabinoid system (ECS) which comprises both CB(1) and CB(2) cannabinoid receptors and their endogenous ligands. The aim of our study was to seek out evidence for the presence of cannabinoid receptors in inflammatory endometrial tissue as well as for their potential role in endometrial inflammation. Our results showed a selective up-regulation of both transcription and expression of CB(2) receptors in biopsies from women affected by endometrial inflammation compared to healthy women. The experiments with the nitric oxide-donor S-Nitroso-L-Glutathione (GSNO) suggest that such a selective up-regulation may be related to the nitric oxide release occurring during endometrial inflammation. In addition, we demonstrated an increase in chymase expression, a marker of mast cells, in biopsies of women affected by endometritis. Therefore our results support the hypothesis that the up-regulation of CB(2) occurs mainly on mast cells and that it might tend to sensitize these cells to the anti-inflammatory effect exerted by endogenous cannabinoids by binding their receptor and thus preventing the mast cell degranulation and the release of pro-inflammatory mediators. In conclusion, we believe that the selective CB(2) up-regulation might play a role as a novel prognostic factor in endometrial inflammation. Blackwell Publishing Ltd 2008-04 2007-07-28 /pmc/articles/PMC3822551/ /pubmed/18419603 http://dx.doi.org/10.1111/j.1582-4934.2007.00085.x Text en ©2008 The Authors Journal compilation © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Images in Cellular / Molecular Medicine
Iuvone, Teresa
De Filippis, Daniele
Di Spiezio Sardo, Attilio
D'Amico, Alessandra
Simonetti, Sara
Sparice, Stefania
Esposito, Giuseppe
Bifulco, Giuseppe
Insabato, Luigi
Nappi, Carmine
Guida, Maurizio
Selective CB2 up-regulation in women affected by endometrial inflammation
title Selective CB2 up-regulation in women affected by endometrial inflammation
title_full Selective CB2 up-regulation in women affected by endometrial inflammation
title_fullStr Selective CB2 up-regulation in women affected by endometrial inflammation
title_full_unstemmed Selective CB2 up-regulation in women affected by endometrial inflammation
title_short Selective CB2 up-regulation in women affected by endometrial inflammation
title_sort selective cb2 up-regulation in women affected by endometrial inflammation
topic Images in Cellular / Molecular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822551/
https://www.ncbi.nlm.nih.gov/pubmed/18419603
http://dx.doi.org/10.1111/j.1582-4934.2007.00085.x
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