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Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function

Epicardial adipose tissue (EAT) has been implicated in the development of heart disease. Nonetheless, the crosstalk between factors secreted from EAT and cardiomyocytes has not been studied. Here, we examined the effect of factors secreted from EAT on contractile function and insulin signalling in p...

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Autores principales: Greulich, Sabrina, de Wiza, Daniella Herzfeld, Preilowski, Sebastian, Ding, Zhaoping, Mueller, Heidi, Langin, Dominique, Jaquet, Kornelia, Ouwens, D Margriet, Eckel, Juergen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822951/
https://www.ncbi.nlm.nih.gov/pubmed/21143387
http://dx.doi.org/10.1111/j.1582-4934.2010.01232.x
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author Greulich, Sabrina
de Wiza, Daniella Herzfeld
Preilowski, Sebastian
Ding, Zhaoping
Mueller, Heidi
Langin, Dominique
Jaquet, Kornelia
Ouwens, D Margriet
Eckel, Juergen
author_facet Greulich, Sabrina
de Wiza, Daniella Herzfeld
Preilowski, Sebastian
Ding, Zhaoping
Mueller, Heidi
Langin, Dominique
Jaquet, Kornelia
Ouwens, D Margriet
Eckel, Juergen
author_sort Greulich, Sabrina
collection PubMed
description Epicardial adipose tissue (EAT) has been implicated in the development of heart disease. Nonetheless, the crosstalk between factors secreted from EAT and cardiomyocytes has not been studied. Here, we examined the effect of factors secreted from EAT on contractile function and insulin signalling in primary rat cardiomocytes. EAT and subcutaneous adipose tissue (SAT) were isolated from guinea pigs fed a high-fat (HFD) or standard diet. HFD feeding for 6 months induced glucose intolerance, and decreased fractional shortening and ejection fraction (all P < 0.05). Conditioned media (CM) generated from EAT and SAT explants were subjected to cytokine profiling using antibody arrays, or incubated with cardiomyocytes to assess the effects on insulin action and contractile function. Eleven factors were differentially secreted by EAT when compared to SAT. Furthermore, secretion of 30 factors by EAT was affected by HFD feeding. Most prominently, activin A-immunoreactivity was 6.4-fold higher in CM from HFD versus standard diet-fed animals and, 2-fold higher in EAT versus SAT. In cardiomyocytes, CM from EAT of HFD-fed animals increased SMAD2-phosphorylation, a marker for activin A-signalling, decreased sarcoplasmic-endoplasmic reticulum calcium ATPase 2a expression, and reduced insulin-mediated phosphorylation of Akt-Ser473 versus CM from SAT and standard diet-fed animals. Finally, CM from EAT of HFD-fed animals as compared to CM from the other groups markedly reduced sarcomere shortening and cytosolic Ca(2+) fluxes in cardiomyocytes. These data provide evidence for an interaction between factors secreted from EAT and cardiomyocyte function.
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spelling pubmed-38229512015-04-06 Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function Greulich, Sabrina de Wiza, Daniella Herzfeld Preilowski, Sebastian Ding, Zhaoping Mueller, Heidi Langin, Dominique Jaquet, Kornelia Ouwens, D Margriet Eckel, Juergen J Cell Mol Med Original Articles Epicardial adipose tissue (EAT) has been implicated in the development of heart disease. Nonetheless, the crosstalk between factors secreted from EAT and cardiomyocytes has not been studied. Here, we examined the effect of factors secreted from EAT on contractile function and insulin signalling in primary rat cardiomocytes. EAT and subcutaneous adipose tissue (SAT) were isolated from guinea pigs fed a high-fat (HFD) or standard diet. HFD feeding for 6 months induced glucose intolerance, and decreased fractional shortening and ejection fraction (all P < 0.05). Conditioned media (CM) generated from EAT and SAT explants were subjected to cytokine profiling using antibody arrays, or incubated with cardiomyocytes to assess the effects on insulin action and contractile function. Eleven factors were differentially secreted by EAT when compared to SAT. Furthermore, secretion of 30 factors by EAT was affected by HFD feeding. Most prominently, activin A-immunoreactivity was 6.4-fold higher in CM from HFD versus standard diet-fed animals and, 2-fold higher in EAT versus SAT. In cardiomyocytes, CM from EAT of HFD-fed animals increased SMAD2-phosphorylation, a marker for activin A-signalling, decreased sarcoplasmic-endoplasmic reticulum calcium ATPase 2a expression, and reduced insulin-mediated phosphorylation of Akt-Ser473 versus CM from SAT and standard diet-fed animals. Finally, CM from EAT of HFD-fed animals as compared to CM from the other groups markedly reduced sarcomere shortening and cytosolic Ca(2+) fluxes in cardiomyocytes. These data provide evidence for an interaction between factors secreted from EAT and cardiomyocyte function. Blackwell Publishing Ltd 2011-11 2011-10-24 /pmc/articles/PMC3822951/ /pubmed/21143387 http://dx.doi.org/10.1111/j.1582-4934.2010.01232.x Text en © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Original Articles
Greulich, Sabrina
de Wiza, Daniella Herzfeld
Preilowski, Sebastian
Ding, Zhaoping
Mueller, Heidi
Langin, Dominique
Jaquet, Kornelia
Ouwens, D Margriet
Eckel, Juergen
Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
title Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
title_full Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
title_fullStr Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
title_full_unstemmed Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
title_short Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
title_sort secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822951/
https://www.ncbi.nlm.nih.gov/pubmed/21143387
http://dx.doi.org/10.1111/j.1582-4934.2010.01232.x
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