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Identification of telocytes in the upper lamina propria of the human urinary tract

The upper lamina propria (ULP) area of interstitial cells (IC) has been studied extensively in bladder, but is rather unexplored in the rest of the urinary tract. This cell layer is intriguing because of the localization directly underneath the urothelium, the intercellular contacts and the close re...

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Autores principales: Gevaert, Thomas, Vos, Rita, Aa, Frank, Joniau, Steven, Oord, Joost, Roskams, Tania, Ridder, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822978/
https://www.ncbi.nlm.nih.gov/pubmed/22151349
http://dx.doi.org/10.1111/j.1582-4934.2011.01504.x
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author Gevaert, Thomas
Vos, Rita
Aa, Frank
Joniau, Steven
Oord, Joost
Roskams, Tania
Ridder, Dirk
author_facet Gevaert, Thomas
Vos, Rita
Aa, Frank
Joniau, Steven
Oord, Joost
Roskams, Tania
Ridder, Dirk
author_sort Gevaert, Thomas
collection PubMed
description The upper lamina propria (ULP) area of interstitial cells (IC) has been studied extensively in bladder, but is rather unexplored in the rest of the urinary tract. This cell layer is intriguing because of the localization directly underneath the urothelium, the intercellular contacts and the close relationship with nerve endings and capillaries. In this study, we examine the ULP layer of IC in human renal pelvis, ureter and urethra, and we make a comparison with ULP IC in bladder. Tissue was obtained from normal areas in nephrectomy, cystectomy and prostatectomy specimens, and processed for morphology, immunohistochemistry and electron microscopy. A morphological and immunohistochemical phenotype for the ULP IC was assessed and region-dependent differences were looked for. The ULP IC in renal pelvis, ureter and urethra had a similar ultrastructural phenotype, which differed somehow from that of bladder IC, that is, thinner and longer cytoplasmic processes, no peripheral actin filaments and presence of dense core granules and microtubules. Together with their immunohistochemical profile, these features are most compatible with the phenotype of telocytes, a recently discovered group of stromal cells. Based on their global ultrastructural and immunohistochemical phenotype, ULP IC in human bladder should also be classified as telocytes. The most striking immunohistochemical finding was the variable expression of oestrogen receptor (ER) and progesterone receptor (PR). The functional relevance of ULP telocytes in the urinary tract remains to be elucidated, and ER and PR might therefore be promising pharmacological research targets.
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spelling pubmed-38229782015-03-27 Identification of telocytes in the upper lamina propria of the human urinary tract Gevaert, Thomas Vos, Rita Aa, Frank Joniau, Steven Oord, Joost Roskams, Tania Ridder, Dirk J Cell Mol Med Original Articles The upper lamina propria (ULP) area of interstitial cells (IC) has been studied extensively in bladder, but is rather unexplored in the rest of the urinary tract. This cell layer is intriguing because of the localization directly underneath the urothelium, the intercellular contacts and the close relationship with nerve endings and capillaries. In this study, we examine the ULP layer of IC in human renal pelvis, ureter and urethra, and we make a comparison with ULP IC in bladder. Tissue was obtained from normal areas in nephrectomy, cystectomy and prostatectomy specimens, and processed for morphology, immunohistochemistry and electron microscopy. A morphological and immunohistochemical phenotype for the ULP IC was assessed and region-dependent differences were looked for. The ULP IC in renal pelvis, ureter and urethra had a similar ultrastructural phenotype, which differed somehow from that of bladder IC, that is, thinner and longer cytoplasmic processes, no peripheral actin filaments and presence of dense core granules and microtubules. Together with their immunohistochemical profile, these features are most compatible with the phenotype of telocytes, a recently discovered group of stromal cells. Based on their global ultrastructural and immunohistochemical phenotype, ULP IC in human bladder should also be classified as telocytes. The most striking immunohistochemical finding was the variable expression of oestrogen receptor (ER) and progesterone receptor (PR). The functional relevance of ULP telocytes in the urinary tract remains to be elucidated, and ER and PR might therefore be promising pharmacological research targets. Blackwell Publishing Ltd 2012-09 2012-08-23 /pmc/articles/PMC3822978/ /pubmed/22151349 http://dx.doi.org/10.1111/j.1582-4934.2011.01504.x Text en Copyright © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
spellingShingle Original Articles
Gevaert, Thomas
Vos, Rita
Aa, Frank
Joniau, Steven
Oord, Joost
Roskams, Tania
Ridder, Dirk
Identification of telocytes in the upper lamina propria of the human urinary tract
title Identification of telocytes in the upper lamina propria of the human urinary tract
title_full Identification of telocytes in the upper lamina propria of the human urinary tract
title_fullStr Identification of telocytes in the upper lamina propria of the human urinary tract
title_full_unstemmed Identification of telocytes in the upper lamina propria of the human urinary tract
title_short Identification of telocytes in the upper lamina propria of the human urinary tract
title_sort identification of telocytes in the upper lamina propria of the human urinary tract
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822978/
https://www.ncbi.nlm.nih.gov/pubmed/22151349
http://dx.doi.org/10.1111/j.1582-4934.2011.01504.x
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