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Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma
The clinical relevance of human leucocyte antigen-G (HLA-G) has been postulated in malignancies. Hepatocellular carcinoma (HCC) is a major contributor to cancer incidence and mortality worldwide; however, potential roles of HLA-G in HCC remain unknown. In the current study, HLA-G expression in 219 p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823007/ https://www.ncbi.nlm.nih.gov/pubmed/19799650 http://dx.doi.org/10.1111/j.1582-4934.2009.00917.x |
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author | Lin, A Chen, H-X Zhu, C-C Zhang, X Xu, H-H Zhang, J-G Wang, Q Zhou, W-J Yan, W-H |
author_facet | Lin, A Chen, H-X Zhu, C-C Zhang, X Xu, H-H Zhang, J-G Wang, Q Zhou, W-J Yan, W-H |
author_sort | Lin, A |
collection | PubMed |
description | The clinical relevance of human leucocyte antigen-G (HLA-G) has been postulated in malignancies. Hepatocellular carcinoma (HCC) is a major contributor to cancer incidence and mortality worldwide; however, potential roles of HLA-G in HCC remain unknown. In the current study, HLA-G expression in 219 primary HCC lesions and their adjacent non-tumourous samples was analysed with immunohistochemistry. Correlations among HLA-G expression and various clinical parameters were evaluated. Meanwhile, functional analysis of transfected cell surface HLA-G expression on NK cell cytolysis was performed in vitro. HLA-G expression was observed in 50.2% (110/219) of primary HCC lesions, and undetectable in corresponding adjacent normal liver tissues. HLA-G expression was found in 37.8%, 41.9% and 71.4% of stage I, II and III HCC lesions, respectively. Data revealed that HLA-G expression in HCC was strongly correlated to advanced disease stage (I versus II, P= 0.882; I versus III, P= 0.020; II versus III, P= 0.037). HLA-G expression was also more frequently observed in elder patients (≥median 52 years, 57.5%versus 43.4%, P= 0.004). Meanwhile, plasma soluble HLA-G in HCC patients was significantly higher than that in normal controls (median, 92.49U/ml versus 9.29U/ml, P= 0.000). Functional assay showed that HLA-G expression in transfected cells could dramatically decrease the NK cell cytolysis (P= 0.036), which could be markedly restored by the blockade of HLA-G (P= 0.004) and its receptor ILT2 (P= 0.019). Our finding indicated that HLA-G expression was strongly correlated to advanced disease stage, and more frequently observed in elder patients. Its relevance to HCC progression might be result from the inhibition of NK cell cytolysis. |
format | Online Article Text |
id | pubmed-3823007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38230072015-04-20 Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma Lin, A Chen, H-X Zhu, C-C Zhang, X Xu, H-H Zhang, J-G Wang, Q Zhou, W-J Yan, W-H J Cell Mol Med Articles The clinical relevance of human leucocyte antigen-G (HLA-G) has been postulated in malignancies. Hepatocellular carcinoma (HCC) is a major contributor to cancer incidence and mortality worldwide; however, potential roles of HLA-G in HCC remain unknown. In the current study, HLA-G expression in 219 primary HCC lesions and their adjacent non-tumourous samples was analysed with immunohistochemistry. Correlations among HLA-G expression and various clinical parameters were evaluated. Meanwhile, functional analysis of transfected cell surface HLA-G expression on NK cell cytolysis was performed in vitro. HLA-G expression was observed in 50.2% (110/219) of primary HCC lesions, and undetectable in corresponding adjacent normal liver tissues. HLA-G expression was found in 37.8%, 41.9% and 71.4% of stage I, II and III HCC lesions, respectively. Data revealed that HLA-G expression in HCC was strongly correlated to advanced disease stage (I versus II, P= 0.882; I versus III, P= 0.020; II versus III, P= 0.037). HLA-G expression was also more frequently observed in elder patients (≥median 52 years, 57.5%versus 43.4%, P= 0.004). Meanwhile, plasma soluble HLA-G in HCC patients was significantly higher than that in normal controls (median, 92.49U/ml versus 9.29U/ml, P= 0.000). Functional assay showed that HLA-G expression in transfected cells could dramatically decrease the NK cell cytolysis (P= 0.036), which could be markedly restored by the blockade of HLA-G (P= 0.004) and its receptor ILT2 (P= 0.019). Our finding indicated that HLA-G expression was strongly correlated to advanced disease stage, and more frequently observed in elder patients. Its relevance to HCC progression might be result from the inhibition of NK cell cytolysis. Blackwell Publishing Ltd 2010-08 2009-10-12 /pmc/articles/PMC3823007/ /pubmed/19799650 http://dx.doi.org/10.1111/j.1582-4934.2009.00917.x Text en © 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Articles Lin, A Chen, H-X Zhu, C-C Zhang, X Xu, H-H Zhang, J-G Wang, Q Zhou, W-J Yan, W-H Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma |
title | Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma |
title_full | Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma |
title_fullStr | Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma |
title_full_unstemmed | Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma |
title_short | Aberrant human leucocyte antigen-G expression and its clinical relevance in hepatocellular carcinoma |
title_sort | aberrant human leucocyte antigen-g expression and its clinical relevance in hepatocellular carcinoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823007/ https://www.ncbi.nlm.nih.gov/pubmed/19799650 http://dx.doi.org/10.1111/j.1582-4934.2009.00917.x |
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