Cargando…
Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium
Angiotensin II (Ang II) has been found to exert preconditioning (PC)–like effect in mammalian hearts. The present investigation reported for the first time a unique mitogen activated protein (MAP) kinase signalling in Ang II PC of the heart involving lipid rafts, which generated a survival signal by...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823257/ https://www.ncbi.nlm.nih.gov/pubmed/17760840 http://dx.doi.org/10.1111/j.1582-4934.2007.00067.x |
_version_ | 1782290539011375104 |
---|---|
author | Das, Manika Das, Samarjit Das, Dipak K |
author_facet | Das, Manika Das, Samarjit Das, Dipak K |
author_sort | Das, Manika |
collection | PubMed |
description | Angiotensin II (Ang II) has been found to exert preconditioning (PC)–like effect in mammalian hearts. The present investigation reported for the first time a unique mitogen activated protein (MAP) kinase signalling in Ang II PC of the heart involving lipid rafts, which generated a survival signal by differentially associating MAP kinases with caveolin. A group of rat hearts was treated with Ang II in the absence or presence of NADPH oxidase inhibitor, apocynin or a cell permeable reactive oxygen species (ROS) scavenger, N-acetyl-cysteine (NAC). Ang II pre-treatment improved post-ischaemic ventricular recovery, myocardial infraction and decreased the number of cardiomyocyte apoptosis indicating PC effect of Ang II. Both apocynin and NAC abolished the PC ability of Ang II. In Ang II treated heart, there was a decreased association of p38MAPKβ & extracellular-signal regulated kinase (ERK) 1/ 2 (anti-death signalling component) with caveolin while there was an increased association of p38MAPKα & Jun N-terminal kinase (JNK) (death signalling component) indicating reduced amount of death signal components and increased amount of anti-death signalling components being available to the Ang II treated heart to generate a survival signal, which was reversed with NAC or apocynin. The survival signal was also demonstrated by increased phosphorylation of serine/threonine-protein kinase B (AKT) and enhanced induction of expression of Bcl-2 during Ang II PC and its reversal with NAC & apocynin treated heart. |
format | Online Article Text |
id | pubmed-3823257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38232572015-04-27 Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium Das, Manika Das, Samarjit Das, Dipak K J Cell Mol Med Articles Angiotensin II (Ang II) has been found to exert preconditioning (PC)–like effect in mammalian hearts. The present investigation reported for the first time a unique mitogen activated protein (MAP) kinase signalling in Ang II PC of the heart involving lipid rafts, which generated a survival signal by differentially associating MAP kinases with caveolin. A group of rat hearts was treated with Ang II in the absence or presence of NADPH oxidase inhibitor, apocynin or a cell permeable reactive oxygen species (ROS) scavenger, N-acetyl-cysteine (NAC). Ang II pre-treatment improved post-ischaemic ventricular recovery, myocardial infraction and decreased the number of cardiomyocyte apoptosis indicating PC effect of Ang II. Both apocynin and NAC abolished the PC ability of Ang II. In Ang II treated heart, there was a decreased association of p38MAPKβ & extracellular-signal regulated kinase (ERK) 1/ 2 (anti-death signalling component) with caveolin while there was an increased association of p38MAPKα & Jun N-terminal kinase (JNK) (death signalling component) indicating reduced amount of death signal components and increased amount of anti-death signalling components being available to the Ang II treated heart to generate a survival signal, which was reversed with NAC or apocynin. The survival signal was also demonstrated by increased phosphorylation of serine/threonine-protein kinase B (AKT) and enhanced induction of expression of Bcl-2 during Ang II PC and its reversal with NAC & apocynin treated heart. Blackwell Publishing Ltd 2007-07 2007-06-24 /pmc/articles/PMC3823257/ /pubmed/17760840 http://dx.doi.org/10.1111/j.1582-4934.2007.00067.x Text en |
spellingShingle | Articles Das, Manika Das, Samarjit Das, Dipak K Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium |
title | Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium |
title_full | Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium |
title_fullStr | Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium |
title_full_unstemmed | Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium |
title_short | Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium |
title_sort | caveolin and map kinase interaction in angiotensin ii preconditioning of the myocardium |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823257/ https://www.ncbi.nlm.nih.gov/pubmed/17760840 http://dx.doi.org/10.1111/j.1582-4934.2007.00067.x |
work_keys_str_mv | AT dasmanika caveolinandmapkinaseinteractioninangiotensiniipreconditioningofthemyocardium AT dassamarjit caveolinandmapkinaseinteractioninangiotensiniipreconditioningofthemyocardium AT dasdipakk caveolinandmapkinaseinteractioninangiotensiniipreconditioningofthemyocardium |