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Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline
Experimental autoimmune neuritis (EAN) is a widely used animal model of the human acute inflammatory demyelinating polyradiculoneuropathy, which is the most common subtype of Guillain-Barré Syndrome. EAN is pathologically characterized by breakdown of the blood-nerve barrier, infiltration of reactiv...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823360/ https://www.ncbi.nlm.nih.gov/pubmed/18400050 http://dx.doi.org/10.1111/j.1582-4934.2008.00333.x |
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author | Zhang, Zhi-Yuan Zhang, Zhiren Fauser, Uwe Schluesener, Hermann J |
author_facet | Zhang, Zhi-Yuan Zhang, Zhiren Fauser, Uwe Schluesener, Hermann J |
author_sort | Zhang, Zhi-Yuan |
collection | PubMed |
description | Experimental autoimmune neuritis (EAN) is a widely used animal model of the human acute inflammatory demyelinating polyradiculoneuropathy, which is the most common subtype of Guillain-Barré Syndrome. EAN is pathologically characterized by breakdown of the blood-nerve barrier, infiltration of reactive immune cells, local inflammation, demyelination in the peripheral nervous system and mechanical allodynia. Minocycline is known to have neuroprotective and anti-inflammatory effects. Furthermore, relieve of neuropathic pain following minocycline administration was observed in a variety of animal models. Here, we investigated the effects of minocycline on rat EAN. Suppressive treatment with minocycline (50 mg/kg body weight daily immediately after immunization) significantly attenuated the severity and duration of EAN. Macrophage and T-cell infiltration and demyelination in sciatic nerves of EAN rats treated with minocycline were significantly reduced compared to phosphate-buffered saline (PBS)-treated EAN rats. mRNA expressions of matrix metallopeptidase-9, inducible nitric oxide synthase and pro-inflammatory cytokines interleukin-1 β and tumour necrosis factor-α in EAN sciatic nerves were greatly decreased by administration of minocycline as well. Furthermore, minocycline attenuated mechanical allodynia in EAN rats and greatly suppressed spinal microglial activation. All together, our data showed that minocycline could effectively suppress the peripheral and spinal inflammation (immune activation) to improve outcome in EAN rats, which suggests that minocycline may be considered as a potential candidate of pharmacological treatment for autoimmune-mediated neuropathies. |
format | Online Article Text |
id | pubmed-3823360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38233602015-04-27 Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline Zhang, Zhi-Yuan Zhang, Zhiren Fauser, Uwe Schluesener, Hermann J J Cell Mol Med Articles Experimental autoimmune neuritis (EAN) is a widely used animal model of the human acute inflammatory demyelinating polyradiculoneuropathy, which is the most common subtype of Guillain-Barré Syndrome. EAN is pathologically characterized by breakdown of the blood-nerve barrier, infiltration of reactive immune cells, local inflammation, demyelination in the peripheral nervous system and mechanical allodynia. Minocycline is known to have neuroprotective and anti-inflammatory effects. Furthermore, relieve of neuropathic pain following minocycline administration was observed in a variety of animal models. Here, we investigated the effects of minocycline on rat EAN. Suppressive treatment with minocycline (50 mg/kg body weight daily immediately after immunization) significantly attenuated the severity and duration of EAN. Macrophage and T-cell infiltration and demyelination in sciatic nerves of EAN rats treated with minocycline were significantly reduced compared to phosphate-buffered saline (PBS)-treated EAN rats. mRNA expressions of matrix metallopeptidase-9, inducible nitric oxide synthase and pro-inflammatory cytokines interleukin-1 β and tumour necrosis factor-α in EAN sciatic nerves were greatly decreased by administration of minocycline as well. Furthermore, minocycline attenuated mechanical allodynia in EAN rats and greatly suppressed spinal microglial activation. All together, our data showed that minocycline could effectively suppress the peripheral and spinal inflammation (immune activation) to improve outcome in EAN rats, which suggests that minocycline may be considered as a potential candidate of pharmacological treatment for autoimmune-mediated neuropathies. Blackwell Publishing Ltd 2009-02 2008-04-08 /pmc/articles/PMC3823360/ /pubmed/18400050 http://dx.doi.org/10.1111/j.1582-4934.2008.00333.x Text en © 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Articles Zhang, Zhi-Yuan Zhang, Zhiren Fauser, Uwe Schluesener, Hermann J Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline |
title | Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline |
title_full | Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline |
title_fullStr | Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline |
title_full_unstemmed | Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline |
title_short | Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline |
title_sort | improved outcome of ean, an animal model of gbs, through amelioration of peripheral and central inflammation by minocycline |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823360/ https://www.ncbi.nlm.nih.gov/pubmed/18400050 http://dx.doi.org/10.1111/j.1582-4934.2008.00333.x |
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