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Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells
The increasing use of induction heating (IH) cooktops has roused public concern in Japan and Europe regarding potential health effects. The purpose of this study was to evaluate the effects of exposure to a magnetic field at 23 kHz (which is the maximum output power frequency of most IH cooktops) on...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823784/ https://www.ncbi.nlm.nih.gov/pubmed/23722077 http://dx.doi.org/10.1093/jrr/rrt063 |
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author | Sakurai, Tomonori Narita, Eijiro Shinohara, Naoki Miyakoshi, Junji |
author_facet | Sakurai, Tomonori Narita, Eijiro Shinohara, Naoki Miyakoshi, Junji |
author_sort | Sakurai, Tomonori |
collection | PubMed |
description | The increasing use of induction heating (IH) cooktops has roused public concern in Japan and Europe regarding potential health effects. The purpose of this study was to evaluate the effects of exposure to a magnetic field at 23 kHz (which is the maximum output power frequency of most IH cooktops) on gene expression in a human-fetus-derived astroglia cell line, SVGp12. The cells were exposed to the magnetic field at 2 mT(rms) [which is approximately 74 times higher than the reference level in the most recent International Commission on Non-Ionizing Radiation Protection (ICNIRP) guidelines], for 2, 4 and 6 h, using a previously reported exposure system. Gene expression was evaluated using an Agilent cDNA microarray. We did not detect any significant effects of the magnetic field on the gene expression profile. On the contrary, heat treatment at 43°C for 2 h used as a positive control significantly affected gene expression, including inducing heat shock proteins, which indicated that our protocol for microarray analysis was appropriate. From these results, we conclude that exposure of human-fetus-derived astroglia cells to an intermediate-frequency magnetic field at 23 kHz and 2 mT(rms) for up to 6 h does not induce detectable alteration of gene expression. |
format | Online Article Text |
id | pubmed-3823784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-38237842013-11-12 Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells Sakurai, Tomonori Narita, Eijiro Shinohara, Naoki Miyakoshi, Junji J Radiat Res Biology The increasing use of induction heating (IH) cooktops has roused public concern in Japan and Europe regarding potential health effects. The purpose of this study was to evaluate the effects of exposure to a magnetic field at 23 kHz (which is the maximum output power frequency of most IH cooktops) on gene expression in a human-fetus-derived astroglia cell line, SVGp12. The cells were exposed to the magnetic field at 2 mT(rms) [which is approximately 74 times higher than the reference level in the most recent International Commission on Non-Ionizing Radiation Protection (ICNIRP) guidelines], for 2, 4 and 6 h, using a previously reported exposure system. Gene expression was evaluated using an Agilent cDNA microarray. We did not detect any significant effects of the magnetic field on the gene expression profile. On the contrary, heat treatment at 43°C for 2 h used as a positive control significantly affected gene expression, including inducing heat shock proteins, which indicated that our protocol for microarray analysis was appropriate. From these results, we conclude that exposure of human-fetus-derived astroglia cells to an intermediate-frequency magnetic field at 23 kHz and 2 mT(rms) for up to 6 h does not induce detectable alteration of gene expression. Oxford University Press 2013-11 2013-05-30 /pmc/articles/PMC3823784/ /pubmed/23722077 http://dx.doi.org/10.1093/jrr/rrt063 Text en © The Author 2013. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biology Sakurai, Tomonori Narita, Eijiro Shinohara, Naoki Miyakoshi, Junji Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells |
title | Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells |
title_full | Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells |
title_fullStr | Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells |
title_full_unstemmed | Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells |
title_short | Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells |
title_sort | alteration of gene expression by exposure to a magnetic field at 23 khz is not detected in astroglia cells |
topic | Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823784/ https://www.ncbi.nlm.nih.gov/pubmed/23722077 http://dx.doi.org/10.1093/jrr/rrt063 |
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