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The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype
Different diseases require different immune responses for efficient protection. Thus, prophylactic vaccines should prime the immune system for the particular type of response needed for protection against a given infectious agent. We have here tested fusion DNA vaccines which encode proteins that bi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823800/ https://www.ncbi.nlm.nih.gov/pubmed/24244595 http://dx.doi.org/10.1371/journal.pone.0080008 |
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author | Grødeland, Gunnveig Mjaaland, Siri Tunheim, Gro Fredriksen, Agnete B. Bogen, Bjarne |
author_facet | Grødeland, Gunnveig Mjaaland, Siri Tunheim, Gro Fredriksen, Agnete B. Bogen, Bjarne |
author_sort | Grødeland, Gunnveig |
collection | PubMed |
description | Different diseases require different immune responses for efficient protection. Thus, prophylactic vaccines should prime the immune system for the particular type of response needed for protection against a given infectious agent. We have here tested fusion DNA vaccines which encode proteins that bivalently target influenza hemagglutinins (HA) to different surface molecules on antigen presenting cells (APC). We demonstrate that targeting to MHC class II molecules predominantly induced an antibody/Th2 response, whereas targeting to CCR1/3/5 predominantly induced a CD8(+)/Th1 T cell response. With respect to antibodies, the polarizing effect was even more pronounced upon intramuscular (i.m) delivery as compared to intradermal (i.d.) vaccination. Despite these differences in induced immune responses, both vaccines protected against a viral challenge with influenza H1N1. Substitution of HA with ovalbumin (OVA) demonstrated that polarization of immune responses, as a consequence of APC targeting specificity, could be extended to other antigens. Taken together, the results demonstrate that vaccination can be tailor-made to induce a particular phenotype of adaptive immune responses by specifically targeting different surface molecules on APCs. |
format | Online Article Text |
id | pubmed-3823800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38238002013-11-15 The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype Grødeland, Gunnveig Mjaaland, Siri Tunheim, Gro Fredriksen, Agnete B. Bogen, Bjarne PLoS One Research Article Different diseases require different immune responses for efficient protection. Thus, prophylactic vaccines should prime the immune system for the particular type of response needed for protection against a given infectious agent. We have here tested fusion DNA vaccines which encode proteins that bivalently target influenza hemagglutinins (HA) to different surface molecules on antigen presenting cells (APC). We demonstrate that targeting to MHC class II molecules predominantly induced an antibody/Th2 response, whereas targeting to CCR1/3/5 predominantly induced a CD8(+)/Th1 T cell response. With respect to antibodies, the polarizing effect was even more pronounced upon intramuscular (i.m) delivery as compared to intradermal (i.d.) vaccination. Despite these differences in induced immune responses, both vaccines protected against a viral challenge with influenza H1N1. Substitution of HA with ovalbumin (OVA) demonstrated that polarization of immune responses, as a consequence of APC targeting specificity, could be extended to other antigens. Taken together, the results demonstrate that vaccination can be tailor-made to induce a particular phenotype of adaptive immune responses by specifically targeting different surface molecules on APCs. Public Library of Science 2013-11-11 /pmc/articles/PMC3823800/ /pubmed/24244595 http://dx.doi.org/10.1371/journal.pone.0080008 Text en © 2013 Grødeland et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Grødeland, Gunnveig Mjaaland, Siri Tunheim, Gro Fredriksen, Agnete B. Bogen, Bjarne The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype |
title | The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype |
title_full | The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype |
title_fullStr | The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype |
title_full_unstemmed | The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype |
title_short | The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype |
title_sort | specificity of targeted vaccines for apc surface molecules influences the immune response phenotype |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823800/ https://www.ncbi.nlm.nih.gov/pubmed/24244595 http://dx.doi.org/10.1371/journal.pone.0080008 |
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