Protein A-Mouse Acidic Mammalian Chitinase-V5-His Expressed in Periplasmic Space of Escherichia coli Possesses Chitinase Functions Comparable to CHO-Expressed Protein

Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fused to Protein A at the N-terminus and V5 epitope a...

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Detalles Bibliográficos
Autores principales: Kashimura, Akinori, Okawa, Kazuaki, Ishikawa, Kotarou, Kida, Yuta, Iwabuchi, Kokoro, Matsushima, Yudai, Sakaguchi, Masayoshi, Sugahara, Yasusato, Oyama, Fumitaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823863/
https://www.ncbi.nlm.nih.gov/pubmed/24244337
http://dx.doi.org/10.1371/journal.pone.0078669

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