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The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice

Retinoids regulate not only various cell functions including proliferation and differentiation but also glucose and lipid metabolism. After we observed a marked up-regulation of cellular retinol-binding protein-I (CRBP-I) in the liver of hepatitis B virus x antigen (HBx)-transgenic (HBx Tg) mice whi...

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Autores principales: Kim, Jo-Heon, Alam, Md. Morshedul, Park, Doek Bae, Cho, Moonjae, Lee, Seung-Hong, Jeon, You-Jin, Yu, Dae-Yeul, Kim, Tae Du, Kim, Ha Young, Cho, Chung Gu, Lee, Dae Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Physiological Society and The Korean Society of Pharmacology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823960/
https://www.ncbi.nlm.nih.gov/pubmed/24227948
http://dx.doi.org/10.4196/kjpp.2013.17.5.455
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author Kim, Jo-Heon
Alam, Md. Morshedul
Park, Doek Bae
Cho, Moonjae
Lee, Seung-Hong
Jeon, You-Jin
Yu, Dae-Yeul
Kim, Tae Du
Kim, Ha Young
Cho, Chung Gu
Lee, Dae Ho
author_facet Kim, Jo-Heon
Alam, Md. Morshedul
Park, Doek Bae
Cho, Moonjae
Lee, Seung-Hong
Jeon, You-Jin
Yu, Dae-Yeul
Kim, Tae Du
Kim, Ha Young
Cho, Chung Gu
Lee, Dae Ho
author_sort Kim, Jo-Heon
collection PubMed
description Retinoids regulate not only various cell functions including proliferation and differentiation but also glucose and lipid metabolism. After we observed a marked up-regulation of cellular retinol-binding protein-I (CRBP-I) in the liver of hepatitis B virus x antigen (HBx)-transgenic (HBx Tg) mice which are prone to hepatocellular carcinoma (HCC) and fatty liver, we aimed to evaluate retinoid pathway, including genes for the retinoid physiology, CRBP-I protein expression, and retinoid levels, in the liver of HBx Tg mice. We also assessed the effect of chronic metformin treatment on HCC development in the mice. Many genes involved in hepatic retinoid physiology, including CRBP-I, were altered and the tissue levels of retinol and all-trans retinoic acid (ATRA) were elevated in the liver of HBx Tg mice compared to those of wild type (WT) control mice. CRBP-I protein expression in liver, but not in white adipose tissue, of HBx Tg mice was significantly elevated compared to WT control mice while CRBP-I protein expressions in the liver and WAT of high-fat fed obese and db/db mice were comparable to WT control mice. Chronic treatment of HBx Tg mice with metformin did not affect the incidence of HCC, but slightly increased hepatic CRBP-I level. In conclusion, hepatic CRBP-I level was markedly up-regulated in HCC-prone HBx Tg mice and neither hepatic CRBP-I nor the development of HCC was suppressed by metformin treatment.
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spelling pubmed-38239602013-11-13 The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice Kim, Jo-Heon Alam, Md. Morshedul Park, Doek Bae Cho, Moonjae Lee, Seung-Hong Jeon, You-Jin Yu, Dae-Yeul Kim, Tae Du Kim, Ha Young Cho, Chung Gu Lee, Dae Ho Korean J Physiol Pharmacol Original Article Retinoids regulate not only various cell functions including proliferation and differentiation but also glucose and lipid metabolism. After we observed a marked up-regulation of cellular retinol-binding protein-I (CRBP-I) in the liver of hepatitis B virus x antigen (HBx)-transgenic (HBx Tg) mice which are prone to hepatocellular carcinoma (HCC) and fatty liver, we aimed to evaluate retinoid pathway, including genes for the retinoid physiology, CRBP-I protein expression, and retinoid levels, in the liver of HBx Tg mice. We also assessed the effect of chronic metformin treatment on HCC development in the mice. Many genes involved in hepatic retinoid physiology, including CRBP-I, were altered and the tissue levels of retinol and all-trans retinoic acid (ATRA) were elevated in the liver of HBx Tg mice compared to those of wild type (WT) control mice. CRBP-I protein expression in liver, but not in white adipose tissue, of HBx Tg mice was significantly elevated compared to WT control mice while CRBP-I protein expressions in the liver and WAT of high-fat fed obese and db/db mice were comparable to WT control mice. Chronic treatment of HBx Tg mice with metformin did not affect the incidence of HCC, but slightly increased hepatic CRBP-I level. In conclusion, hepatic CRBP-I level was markedly up-regulated in HCC-prone HBx Tg mice and neither hepatic CRBP-I nor the development of HCC was suppressed by metformin treatment. The Korean Physiological Society and The Korean Society of Pharmacology 2013-10 2013-10-17 /pmc/articles/PMC3823960/ /pubmed/24227948 http://dx.doi.org/10.4196/kjpp.2013.17.5.455 Text en Copyright © 2013 The Korean Physiological Society and The Korean Society of Pharmacology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Jo-Heon
Alam, Md. Morshedul
Park, Doek Bae
Cho, Moonjae
Lee, Seung-Hong
Jeon, You-Jin
Yu, Dae-Yeul
Kim, Tae Du
Kim, Ha Young
Cho, Chung Gu
Lee, Dae Ho
The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice
title The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice
title_full The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice
title_fullStr The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice
title_full_unstemmed The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice
title_short The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice
title_sort effect of metformin treatment on crbp-i level and cancer development in the liver of hbx transgenic mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823960/
https://www.ncbi.nlm.nih.gov/pubmed/24227948
http://dx.doi.org/10.4196/kjpp.2013.17.5.455
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