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The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824018/ https://www.ncbi.nlm.nih.gov/pubmed/24217623 http://dx.doi.org/10.1083/jcb.201306036 |
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author | Green, Rebecca A. Mayers, Jonathan R. Wang, Shaohe Lewellyn, Lindsay Desai, Arshad Audhya, Anjon Oegema, Karen |
author_facet | Green, Rebecca A. Mayers, Jonathan R. Wang, Shaohe Lewellyn, Lindsay Desai, Arshad Audhya, Anjon Oegema, Karen |
author_sort | Green, Rebecca A. |
collection | PubMed |
description | Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery. In this paper, we assess the contribution of midbody microtubules versus the midbody ring in the Caenorhabditis elegans embryo. We show that abscission occurs in two stages. First, the cytoplasm in the daughter cells becomes isolated, coincident with formation of the intercellular bridge; proper progression through this stage required the septins (a midbody ring component) but not the membrane-remodeling endosomal sorting complex required for transport (ESCRT) machinery. Second, the midbody and midbody ring are released into a specific daughter cell during the subsequent cell division; this stage required the septins and the ESCRT machinery. Surprisingly, midbody microtubules were dispensable for both stages. These results delineate distinct steps during abscission and highlight the central role of the midbody ring, rather than midbody microtubules, in their execution. |
format | Online Article Text |
id | pubmed-3824018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-38240182014-05-11 The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules Green, Rebecca A. Mayers, Jonathan R. Wang, Shaohe Lewellyn, Lindsay Desai, Arshad Audhya, Anjon Oegema, Karen J Cell Biol Research Articles Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery. In this paper, we assess the contribution of midbody microtubules versus the midbody ring in the Caenorhabditis elegans embryo. We show that abscission occurs in two stages. First, the cytoplasm in the daughter cells becomes isolated, coincident with formation of the intercellular bridge; proper progression through this stage required the septins (a midbody ring component) but not the membrane-remodeling endosomal sorting complex required for transport (ESCRT) machinery. Second, the midbody and midbody ring are released into a specific daughter cell during the subsequent cell division; this stage required the septins and the ESCRT machinery. Surprisingly, midbody microtubules were dispensable for both stages. These results delineate distinct steps during abscission and highlight the central role of the midbody ring, rather than midbody microtubules, in their execution. The Rockefeller University Press 2013-11-11 /pmc/articles/PMC3824018/ /pubmed/24217623 http://dx.doi.org/10.1083/jcb.201306036 Text en © 2013 Green et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Green, Rebecca A. Mayers, Jonathan R. Wang, Shaohe Lewellyn, Lindsay Desai, Arshad Audhya, Anjon Oegema, Karen The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
title | The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
title_full | The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
title_fullStr | The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
title_full_unstemmed | The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
title_short | The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
title_sort | midbody ring scaffolds the abscission machinery in the absence of midbody microtubules |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824018/ https://www.ncbi.nlm.nih.gov/pubmed/24217623 http://dx.doi.org/10.1083/jcb.201306036 |
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