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The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules

Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery....

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Autores principales: Green, Rebecca A., Mayers, Jonathan R., Wang, Shaohe, Lewellyn, Lindsay, Desai, Arshad, Audhya, Anjon, Oegema, Karen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824018/
https://www.ncbi.nlm.nih.gov/pubmed/24217623
http://dx.doi.org/10.1083/jcb.201306036
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author Green, Rebecca A.
Mayers, Jonathan R.
Wang, Shaohe
Lewellyn, Lindsay
Desai, Arshad
Audhya, Anjon
Oegema, Karen
author_facet Green, Rebecca A.
Mayers, Jonathan R.
Wang, Shaohe
Lewellyn, Lindsay
Desai, Arshad
Audhya, Anjon
Oegema, Karen
author_sort Green, Rebecca A.
collection PubMed
description Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery. In this paper, we assess the contribution of midbody microtubules versus the midbody ring in the Caenorhabditis elegans embryo. We show that abscission occurs in two stages. First, the cytoplasm in the daughter cells becomes isolated, coincident with formation of the intercellular bridge; proper progression through this stage required the septins (a midbody ring component) but not the membrane-remodeling endosomal sorting complex required for transport (ESCRT) machinery. Second, the midbody and midbody ring are released into a specific daughter cell during the subsequent cell division; this stage required the septins and the ESCRT machinery. Surprisingly, midbody microtubules were dispensable for both stages. These results delineate distinct steps during abscission and highlight the central role of the midbody ring, rather than midbody microtubules, in their execution.
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spelling pubmed-38240182014-05-11 The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules Green, Rebecca A. Mayers, Jonathan R. Wang, Shaohe Lewellyn, Lindsay Desai, Arshad Audhya, Anjon Oegema, Karen J Cell Biol Research Articles Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring–derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery. In this paper, we assess the contribution of midbody microtubules versus the midbody ring in the Caenorhabditis elegans embryo. We show that abscission occurs in two stages. First, the cytoplasm in the daughter cells becomes isolated, coincident with formation of the intercellular bridge; proper progression through this stage required the septins (a midbody ring component) but not the membrane-remodeling endosomal sorting complex required for transport (ESCRT) machinery. Second, the midbody and midbody ring are released into a specific daughter cell during the subsequent cell division; this stage required the septins and the ESCRT machinery. Surprisingly, midbody microtubules were dispensable for both stages. These results delineate distinct steps during abscission and highlight the central role of the midbody ring, rather than midbody microtubules, in their execution. The Rockefeller University Press 2013-11-11 /pmc/articles/PMC3824018/ /pubmed/24217623 http://dx.doi.org/10.1083/jcb.201306036 Text en © 2013 Green et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Green, Rebecca A.
Mayers, Jonathan R.
Wang, Shaohe
Lewellyn, Lindsay
Desai, Arshad
Audhya, Anjon
Oegema, Karen
The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
title The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
title_full The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
title_fullStr The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
title_full_unstemmed The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
title_short The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
title_sort midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824018/
https://www.ncbi.nlm.nih.gov/pubmed/24217623
http://dx.doi.org/10.1083/jcb.201306036
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