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The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
The Calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein is highly expressed in mammalian brain as well as in neuroblastoma NB2a cells and pheochromocytoma PC12 cells. This protein interacts with several targets such as cytoskeletal proteins or ERK1/2 kinase and seems to be...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824344/ https://www.ncbi.nlm.nih.gov/pubmed/24078263 http://dx.doi.org/10.1007/s11064-013-1155-4 |
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author | Wasik, Urszula Filipek, Anna |
author_facet | Wasik, Urszula Filipek, Anna |
author_sort | Wasik, Urszula |
collection | PubMed |
description | The Calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein is highly expressed in mammalian brain as well as in neuroblastoma NB2a cells and pheochromocytoma PC12 cells. This protein interacts with several targets such as cytoskeletal proteins or ERK1/2 kinase and seems to be involved in many cellular processes. In this work we examined a post-translational modification of CacyBP/SIP which might have an effect on its function. Since theoretical analysis of the amino acid sequence of CacyBP/SIP indicated several lysine residues which could potentially be sumoylated we checked experimentally whether this protein might be modified by SUMO attachment. We have shown that indeed CacyBP/SIP bound the E2 SUMO ligase, Ubc9, in neuroblastoma NB2a cell extract and was sumoylated in these cells. By fractionation of NB2a cell extract we have found that, contrary to the majority of SUMO-modified proteins, sumoylated CacyBP/SIP is present in the cytoplasmic and not in the nuclear fraction. We have also established that lysine 16 is the residue which undergoes sumoylation in the CacyBP/SIP protein. |
format | Online Article Text |
id | pubmed-3824344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-38243442013-11-21 The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells Wasik, Urszula Filipek, Anna Neurochem Res Original Paper The Calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein is highly expressed in mammalian brain as well as in neuroblastoma NB2a cells and pheochromocytoma PC12 cells. This protein interacts with several targets such as cytoskeletal proteins or ERK1/2 kinase and seems to be involved in many cellular processes. In this work we examined a post-translational modification of CacyBP/SIP which might have an effect on its function. Since theoretical analysis of the amino acid sequence of CacyBP/SIP indicated several lysine residues which could potentially be sumoylated we checked experimentally whether this protein might be modified by SUMO attachment. We have shown that indeed CacyBP/SIP bound the E2 SUMO ligase, Ubc9, in neuroblastoma NB2a cell extract and was sumoylated in these cells. By fractionation of NB2a cell extract we have found that, contrary to the majority of SUMO-modified proteins, sumoylated CacyBP/SIP is present in the cytoplasmic and not in the nuclear fraction. We have also established that lysine 16 is the residue which undergoes sumoylation in the CacyBP/SIP protein. Springer US 2013-09-28 2013 /pmc/articles/PMC3824344/ /pubmed/24078263 http://dx.doi.org/10.1007/s11064-013-1155-4 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Paper Wasik, Urszula Filipek, Anna The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells |
title | The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells |
title_full | The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells |
title_fullStr | The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells |
title_full_unstemmed | The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells |
title_short | The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells |
title_sort | cacybp/sip protein is sumoylated in neuroblastoma nb2a cells |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824344/ https://www.ncbi.nlm.nih.gov/pubmed/24078263 http://dx.doi.org/10.1007/s11064-013-1155-4 |
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