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The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

The Calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein is highly expressed in mammalian brain as well as in neuroblastoma NB2a cells and pheochromocytoma PC12 cells. This protein interacts with several targets such as cytoskeletal proteins or ERK1/2 kinase and seems to be...

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Autores principales: Wasik, Urszula, Filipek, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824344/
https://www.ncbi.nlm.nih.gov/pubmed/24078263
http://dx.doi.org/10.1007/s11064-013-1155-4
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author Wasik, Urszula
Filipek, Anna
author_facet Wasik, Urszula
Filipek, Anna
author_sort Wasik, Urszula
collection PubMed
description The Calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein is highly expressed in mammalian brain as well as in neuroblastoma NB2a cells and pheochromocytoma PC12 cells. This protein interacts with several targets such as cytoskeletal proteins or ERK1/2 kinase and seems to be involved in many cellular processes. In this work we examined a post-translational modification of CacyBP/SIP which might have an effect on its function. Since theoretical analysis of the amino acid sequence of CacyBP/SIP indicated several lysine residues which could potentially be sumoylated we checked experimentally whether this protein might be modified by SUMO attachment. We have shown that indeed CacyBP/SIP bound the E2 SUMO ligase, Ubc9, in neuroblastoma NB2a cell extract and was sumoylated in these cells. By fractionation of NB2a cell extract we have found that, contrary to the majority of SUMO-modified proteins, sumoylated CacyBP/SIP is present in the cytoplasmic and not in the nuclear fraction. We have also established that lysine 16 is the residue which undergoes sumoylation in the CacyBP/SIP protein.
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spelling pubmed-38243442013-11-21 The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells Wasik, Urszula Filipek, Anna Neurochem Res Original Paper The Calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein is highly expressed in mammalian brain as well as in neuroblastoma NB2a cells and pheochromocytoma PC12 cells. This protein interacts with several targets such as cytoskeletal proteins or ERK1/2 kinase and seems to be involved in many cellular processes. In this work we examined a post-translational modification of CacyBP/SIP which might have an effect on its function. Since theoretical analysis of the amino acid sequence of CacyBP/SIP indicated several lysine residues which could potentially be sumoylated we checked experimentally whether this protein might be modified by SUMO attachment. We have shown that indeed CacyBP/SIP bound the E2 SUMO ligase, Ubc9, in neuroblastoma NB2a cell extract and was sumoylated in these cells. By fractionation of NB2a cell extract we have found that, contrary to the majority of SUMO-modified proteins, sumoylated CacyBP/SIP is present in the cytoplasmic and not in the nuclear fraction. We have also established that lysine 16 is the residue which undergoes sumoylation in the CacyBP/SIP protein. Springer US 2013-09-28 2013 /pmc/articles/PMC3824344/ /pubmed/24078263 http://dx.doi.org/10.1007/s11064-013-1155-4 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Wasik, Urszula
Filipek, Anna
The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
title The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
title_full The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
title_fullStr The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
title_full_unstemmed The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
title_short The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells
title_sort cacybp/sip protein is sumoylated in neuroblastoma nb2a cells
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824344/
https://www.ncbi.nlm.nih.gov/pubmed/24078263
http://dx.doi.org/10.1007/s11064-013-1155-4
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