Mutational Analysis of cj0183 Campylobacter jejuni Promoter

Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragment...

Descripción completa

Detalles Bibliográficos
Autores principales: Salamasznska-Guz, Agnieszka, Grodzik, Marta, Klimuszko, Danuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824568/
https://www.ncbi.nlm.nih.gov/pubmed/23884593
http://dx.doi.org/10.1007/s00284-013-0420-8
_version_ 1782290714360545280
author Salamasznska-Guz, Agnieszka
Grodzik, Marta
Klimuszko, Danuta
author_facet Salamasznska-Guz, Agnieszka
Grodzik, Marta
Klimuszko, Danuta
author_sort Salamasznska-Guz, Agnieszka
collection PubMed
description Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene. The β-galactosidase activity determined for the strain harboring the plasmid with the fragment upstream of cj0183 indicated the presence of a promoter in this DNA region. Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription.
format Online
Article
Text
id pubmed-3824568
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-38245682013-11-21 Mutational Analysis of cj0183 Campylobacter jejuni Promoter Salamasznska-Guz, Agnieszka Grodzik, Marta Klimuszko, Danuta Curr Microbiol Article Gene-nominated cj0183 was identified in Campylobacter jejuni NCTC 11168 and in two human isolates 81116 and 81-176. It encodes a protein which shows partial homology to TlyC of Brachyspira hyodysenteriae. The aim of this work was to determine the mechanisms of gene regulation by cloning DNA fragments lying upstream of the cj0183 gene. The β-galactosidase activity determined for the strain harboring the plasmid with the fragment upstream of cj0183 indicated the presence of a promoter in this DNA region. Mutations in cj0183 -10 region, -16 region, and -35 region resulted in changes in gene transcription. Springer US 2013-07-25 2013 /pmc/articles/PMC3824568/ /pubmed/23884593 http://dx.doi.org/10.1007/s00284-013-0420-8 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Salamasznska-Guz, Agnieszka
Grodzik, Marta
Klimuszko, Danuta
Mutational Analysis of cj0183 Campylobacter jejuni Promoter
title Mutational Analysis of cj0183 Campylobacter jejuni Promoter
title_full Mutational Analysis of cj0183 Campylobacter jejuni Promoter
title_fullStr Mutational Analysis of cj0183 Campylobacter jejuni Promoter
title_full_unstemmed Mutational Analysis of cj0183 Campylobacter jejuni Promoter
title_short Mutational Analysis of cj0183 Campylobacter jejuni Promoter
title_sort mutational analysis of cj0183 campylobacter jejuni promoter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824568/
https://www.ncbi.nlm.nih.gov/pubmed/23884593
http://dx.doi.org/10.1007/s00284-013-0420-8
work_keys_str_mv AT salamasznskaguzagnieszka mutationalanalysisofcj0183campylobacterjejunipromoter
AT grodzikmarta mutationalanalysisofcj0183campylobacterjejunipromoter
AT klimuszkodanuta mutationalanalysisofcj0183campylobacterjejunipromoter

Ejemplares similares