Cargando…

Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs

In this work, inulinase was produced by solid state fermentation by Streptomyces sp. using copra waste as carbon source. The nutrients were screened by Plackett–Burman design. From the pareto chart it was found that the nutrients, namely, soya bean cake, MgSO(4)·7H(2)O and (NH(4))(2)SO(4) were found...

Descripción completa

Detalles Bibliográficos
Autores principales: Dilipkumar, M., Rajasimman, M., Rajamohan, N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824788/
https://www.ncbi.nlm.nih.gov/pubmed/28324419
http://dx.doi.org/10.1007/s13205-012-0112-2
_version_ 1782290744590991360
author Dilipkumar, M.
Rajasimman, M.
Rajamohan, N.
author_facet Dilipkumar, M.
Rajasimman, M.
Rajamohan, N.
author_sort Dilipkumar, M.
collection PubMed
description In this work, inulinase was produced by solid state fermentation by Streptomyces sp. using copra waste as carbon source. The nutrients were screened by Plackett–Burman design. From the pareto chart it was found that the nutrients, namely, soya bean cake, MgSO(4)·7H(2)O and (NH(4))(2)SO(4) were found to be most significant nutrient components. Hence, these three components were selected for further optimization using central composite design (CCD) in response surface methodology (RSM). The optimum conditions were soya bean cake: 0.05711 g/gds, MgSO(4)·7H(2)O: 0.00063 g/gds and (NH(4))(2)SO(4): 0.00772 g/gds. Under these optimized conditions, the production of inulinase was found to be 131 U/gds. The constants in the Michaelis–Menten equation were evaluated and high R(2) value implies the fitness of the model.
format Online
Article
Text
id pubmed-3824788
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-38247882013-11-19 Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs Dilipkumar, M. Rajasimman, M. Rajamohan, N. 3 Biotech Original Article In this work, inulinase was produced by solid state fermentation by Streptomyces sp. using copra waste as carbon source. The nutrients were screened by Plackett–Burman design. From the pareto chart it was found that the nutrients, namely, soya bean cake, MgSO(4)·7H(2)O and (NH(4))(2)SO(4) were found to be most significant nutrient components. Hence, these three components were selected for further optimization using central composite design (CCD) in response surface methodology (RSM). The optimum conditions were soya bean cake: 0.05711 g/gds, MgSO(4)·7H(2)O: 0.00063 g/gds and (NH(4))(2)SO(4): 0.00772 g/gds. Under these optimized conditions, the production of inulinase was found to be 131 U/gds. The constants in the Michaelis–Menten equation were evaluated and high R(2) value implies the fitness of the model. Springer Berlin Heidelberg 2013-01-03 2013-12 /pmc/articles/PMC3824788/ /pubmed/28324419 http://dx.doi.org/10.1007/s13205-012-0112-2 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/2.0/ This article is published under license to BioMed Central Ltd. Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Dilipkumar, M.
Rajasimman, M.
Rajamohan, N.
Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs
title Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs
title_full Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs
title_fullStr Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs
title_full_unstemmed Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs
title_short Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs
title_sort enhanced inulinase production by streptomyces sp. in solid state fermentation through statistical designs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824788/
https://www.ncbi.nlm.nih.gov/pubmed/28324419
http://dx.doi.org/10.1007/s13205-012-0112-2
work_keys_str_mv AT dilipkumarm enhancedinulinaseproductionbystreptomycesspinsolidstatefermentationthroughstatisticaldesigns
AT rajasimmanm enhancedinulinaseproductionbystreptomycesspinsolidstatefermentationthroughstatisticaldesigns
AT rajamohann enhancedinulinaseproductionbystreptomycesspinsolidstatefermentationthroughstatisticaldesigns