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Phospholipid composition and longevity: lessons from Ames dwarf mice

Membrane fatty acid (FA) composition is correlated with longevity in mammals. The “membrane pacemaker hypothesis of ageing” proposes that animals which cellular membranes contain high amounts of polyunsaturated FAs (PUFAs) have shorter life spans because their membranes are more susceptible to perox...

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Autores principales: Valencak, Teresa G., Ruf, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825011/
https://www.ncbi.nlm.nih.gov/pubmed/23640425
http://dx.doi.org/10.1007/s11357-013-9533-z
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author Valencak, Teresa G.
Ruf, Thomas
author_facet Valencak, Teresa G.
Ruf, Thomas
author_sort Valencak, Teresa G.
collection PubMed
description Membrane fatty acid (FA) composition is correlated with longevity in mammals. The “membrane pacemaker hypothesis of ageing” proposes that animals which cellular membranes contain high amounts of polyunsaturated FAs (PUFAs) have shorter life spans because their membranes are more susceptible to peroxidation and further oxidative damage. It remains to be shown, however, that long-lived phenotypes such as the Ames dwarf mouse have membranes containing fewer PUFAs and thus being less prone to peroxidation, as would be predicted from the membrane pacemaker hypothesis of ageing. Here, we show that across four different tissues, i.e., muscle, heart, liver and brain as well as in liver mitochondria, Ames dwarf mice possess membrane phospholipids containing between 30 and 60 % PUFAs (depending on the tissue), which is similar to PUFA contents of their normal-sized, short-lived siblings. However, we found that that Ames dwarf mice membrane phospholipids were significantly poorer in n-3 PUFAs. While lack of a difference in PUFA contents is contradicting the membrane pacemaker hypothesis, the lower n-3 PUFAs content in the long-lived mice provides some support for the membrane pacemaker hypothesis of ageing, as n-3 PUFAs comprise those FAs being blamed most for causing oxidative damage. By comparing tissue composition between 1-, 2- and 6-month-old mice in both phenotypes, we found that membranes differed both in quantity of PUFAs and in the prevalence of certain PUFAs. In sum, membrane composition in the Ames dwarf mouse supports the concept that tissue FA composition is related to longevity.
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spelling pubmed-38250112013-11-25 Phospholipid composition and longevity: lessons from Ames dwarf mice Valencak, Teresa G. Ruf, Thomas Age (Dordr) Article Membrane fatty acid (FA) composition is correlated with longevity in mammals. The “membrane pacemaker hypothesis of ageing” proposes that animals which cellular membranes contain high amounts of polyunsaturated FAs (PUFAs) have shorter life spans because their membranes are more susceptible to peroxidation and further oxidative damage. It remains to be shown, however, that long-lived phenotypes such as the Ames dwarf mouse have membranes containing fewer PUFAs and thus being less prone to peroxidation, as would be predicted from the membrane pacemaker hypothesis of ageing. Here, we show that across four different tissues, i.e., muscle, heart, liver and brain as well as in liver mitochondria, Ames dwarf mice possess membrane phospholipids containing between 30 and 60 % PUFAs (depending on the tissue), which is similar to PUFA contents of their normal-sized, short-lived siblings. However, we found that that Ames dwarf mice membrane phospholipids were significantly poorer in n-3 PUFAs. While lack of a difference in PUFA contents is contradicting the membrane pacemaker hypothesis, the lower n-3 PUFAs content in the long-lived mice provides some support for the membrane pacemaker hypothesis of ageing, as n-3 PUFAs comprise those FAs being blamed most for causing oxidative damage. By comparing tissue composition between 1-, 2- and 6-month-old mice in both phenotypes, we found that membranes differed both in quantity of PUFAs and in the prevalence of certain PUFAs. In sum, membrane composition in the Ames dwarf mouse supports the concept that tissue FA composition is related to longevity. Springer Netherlands 2013-05-03 2013-12 /pmc/articles/PMC3825011/ /pubmed/23640425 http://dx.doi.org/10.1007/s11357-013-9533-z Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Valencak, Teresa G.
Ruf, Thomas
Phospholipid composition and longevity: lessons from Ames dwarf mice
title Phospholipid composition and longevity: lessons from Ames dwarf mice
title_full Phospholipid composition and longevity: lessons from Ames dwarf mice
title_fullStr Phospholipid composition and longevity: lessons from Ames dwarf mice
title_full_unstemmed Phospholipid composition and longevity: lessons from Ames dwarf mice
title_short Phospholipid composition and longevity: lessons from Ames dwarf mice
title_sort phospholipid composition and longevity: lessons from ames dwarf mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825011/
https://www.ncbi.nlm.nih.gov/pubmed/23640425
http://dx.doi.org/10.1007/s11357-013-9533-z
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