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Transport Reversal during Heteroexchange: A Kinetic Study
It is known that secondary transporters, which utilize transmembrane ionic gradients to drive their substrates up a concentration gradient, can reverse the uptake and instead release their substrates. Unfortunately, the Michaelis-Menten kinetic scheme, which is popular in transporter studies, does n...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825127/ https://www.ncbi.nlm.nih.gov/pubmed/24307897 http://dx.doi.org/10.1155/2013/683256 |
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author | Makarov, V. Kucheryavykh, L. Kucheryavykh, Y. Rivera, A. Eaton, M. J. Skatchkov, S. N. Inyushin, M. |
author_facet | Makarov, V. Kucheryavykh, L. Kucheryavykh, Y. Rivera, A. Eaton, M. J. Skatchkov, S. N. Inyushin, M. |
author_sort | Makarov, V. |
collection | PubMed |
description | It is known that secondary transporters, which utilize transmembrane ionic gradients to drive their substrates up a concentration gradient, can reverse the uptake and instead release their substrates. Unfortunately, the Michaelis-Menten kinetic scheme, which is popular in transporter studies, does not include transporter reversal, and it completely neglects the possibility of equilibrium between the substrate concentrations on both sides of the membrane. We have developed a complex two-substrate kinetic model that includes transport reversal. This model allows us to construct analytical formulas allowing the calculation of a “heteroexchange” and “transacceleration” using standard Michaelis coefficients for respective substrates. This approach can help to understand how glial and other cells accumulate substrates without synthesis and are able to release such substrates and gliotransmitters. |
format | Online Article Text |
id | pubmed-3825127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38251272013-12-04 Transport Reversal during Heteroexchange: A Kinetic Study Makarov, V. Kucheryavykh, L. Kucheryavykh, Y. Rivera, A. Eaton, M. J. Skatchkov, S. N. Inyushin, M. J Biophys Research Article It is known that secondary transporters, which utilize transmembrane ionic gradients to drive their substrates up a concentration gradient, can reverse the uptake and instead release their substrates. Unfortunately, the Michaelis-Menten kinetic scheme, which is popular in transporter studies, does not include transporter reversal, and it completely neglects the possibility of equilibrium between the substrate concentrations on both sides of the membrane. We have developed a complex two-substrate kinetic model that includes transport reversal. This model allows us to construct analytical formulas allowing the calculation of a “heteroexchange” and “transacceleration” using standard Michaelis coefficients for respective substrates. This approach can help to understand how glial and other cells accumulate substrates without synthesis and are able to release such substrates and gliotransmitters. Hindawi Publishing Corporation 2013 2013-10-26 /pmc/articles/PMC3825127/ /pubmed/24307897 http://dx.doi.org/10.1155/2013/683256 Text en Copyright © 2013 V. Makarov et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Makarov, V. Kucheryavykh, L. Kucheryavykh, Y. Rivera, A. Eaton, M. J. Skatchkov, S. N. Inyushin, M. Transport Reversal during Heteroexchange: A Kinetic Study |
title | Transport Reversal during Heteroexchange: A Kinetic Study |
title_full | Transport Reversal during Heteroexchange: A Kinetic Study |
title_fullStr | Transport Reversal during Heteroexchange: A Kinetic Study |
title_full_unstemmed | Transport Reversal during Heteroexchange: A Kinetic Study |
title_short | Transport Reversal during Heteroexchange: A Kinetic Study |
title_sort | transport reversal during heteroexchange: a kinetic study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825127/ https://www.ncbi.nlm.nih.gov/pubmed/24307897 http://dx.doi.org/10.1155/2013/683256 |
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