Cargando…

Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.

The physiological, biochemical and molecular mechanisms regulating the initiation of a regenerative pathway remain partially unknown. Efforts to identify the biological features that confer transformation ability, or the tendency of some cells to induce transgene silencing, would help to improve pla...

Descripción completa

Detalles Bibliográficos
Autores principales: Delporte, Fabienne, Muhovski, Yordan, Pretova, Anna, Watillon, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825128/
https://www.ncbi.nlm.nih.gov/pubmed/24078158
http://dx.doi.org/10.1007/s11033-013-2696-y
_version_ 1782290770872500224
author Delporte, Fabienne
Muhovski, Yordan
Pretova, Anna
Watillon, Bernard
author_facet Delporte, Fabienne
Muhovski, Yordan
Pretova, Anna
Watillon, Bernard
author_sort Delporte, Fabienne
collection PubMed
description The physiological, biochemical and molecular mechanisms regulating the initiation of a regenerative pathway remain partially unknown. Efforts to identify the biological features that confer transformation ability, or the tendency of some cells to induce transgene silencing, would help to improve plant genetic engineering. The objective of our study was to monitor the evolution of plant cell competencies in relation to both in vitro tissue culture regeneration and the genetic transformation properties. We used a simple wheat regeneration procedure as an experimental model for studying the regenerative capacity of plant cells and their receptivity to direct gene transfer over the successive steps of the regenerative pathway. Target gene profiling studies and biochemical assays were conducted to follow some of the mechanisms triggered during the somatic-to-embryogenic transition (i.e. dedifferentiation, cell division activation, redifferentiation) and affecting the accessibility of plant cells to receive and stably express the exogenous DNA introduced by bombardment. Our results seem to indicate that the control of cell-cycle (S-phase) and host defense strategies can be crucial determinants of genetic transformation efficiency. The results from studies conducted at macro-, micro- and molecular scales are then integrated into a holistic approach that addresses the question of tissue culture and transgenesis competencies more broadly. Through this multilevel analysis we try to establish functional links between both regenerative capacity and transformation receptiveness, and thereby to provide a more global and integrated vision of both processes, at the core of defense/adaptive mechanisms and survival, between undifferentiated cell proliferation and organization. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11033-013-2696-y) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-3825128
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-38251282013-11-21 Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L. Delporte, Fabienne Muhovski, Yordan Pretova, Anna Watillon, Bernard Mol Biol Rep Article The physiological, biochemical and molecular mechanisms regulating the initiation of a regenerative pathway remain partially unknown. Efforts to identify the biological features that confer transformation ability, or the tendency of some cells to induce transgene silencing, would help to improve plant genetic engineering. The objective of our study was to monitor the evolution of plant cell competencies in relation to both in vitro tissue culture regeneration and the genetic transformation properties. We used a simple wheat regeneration procedure as an experimental model for studying the regenerative capacity of plant cells and their receptivity to direct gene transfer over the successive steps of the regenerative pathway. Target gene profiling studies and biochemical assays were conducted to follow some of the mechanisms triggered during the somatic-to-embryogenic transition (i.e. dedifferentiation, cell division activation, redifferentiation) and affecting the accessibility of plant cells to receive and stably express the exogenous DNA introduced by bombardment. Our results seem to indicate that the control of cell-cycle (S-phase) and host defense strategies can be crucial determinants of genetic transformation efficiency. The results from studies conducted at macro-, micro- and molecular scales are then integrated into a holistic approach that addresses the question of tissue culture and transgenesis competencies more broadly. Through this multilevel analysis we try to establish functional links between both regenerative capacity and transformation receptiveness, and thereby to provide a more global and integrated vision of both processes, at the core of defense/adaptive mechanisms and survival, between undifferentiated cell proliferation and organization. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11033-013-2696-y) contains supplementary material, which is available to authorized users. Springer Netherlands 2013-09-29 2013 /pmc/articles/PMC3825128/ /pubmed/24078158 http://dx.doi.org/10.1007/s11033-013-2696-y Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Delporte, Fabienne
Muhovski, Yordan
Pretova, Anna
Watillon, Bernard
Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.
title Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.
title_full Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.
title_fullStr Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.
title_full_unstemmed Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.
title_short Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.
title_sort analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in triticum aestivum l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825128/
https://www.ncbi.nlm.nih.gov/pubmed/24078158
http://dx.doi.org/10.1007/s11033-013-2696-y
work_keys_str_mv AT delportefabienne analysisofexpressionprofilesofselectedgenesassociatedwiththeregenerativepropertyandthereceptivitytogenetransferduringsomaticembryogenesisintriticumaestivuml
AT muhovskiyordan analysisofexpressionprofilesofselectedgenesassociatedwiththeregenerativepropertyandthereceptivitytogenetransferduringsomaticembryogenesisintriticumaestivuml
AT pretovaanna analysisofexpressionprofilesofselectedgenesassociatedwiththeregenerativepropertyandthereceptivitytogenetransferduringsomaticembryogenesisintriticumaestivuml
AT watillonbernard analysisofexpressionprofilesofselectedgenesassociatedwiththeregenerativepropertyandthereceptivitytogenetransferduringsomaticembryogenesisintriticumaestivuml