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Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens
Toll-like receptors (TLRs) are members of the cellular receptors that constitute a major component of the evolutionary conserved pattern recognition system (PRR). TLRs are expressed in a wide variety of tissues and cell types. In this study we compared the expression profiles of the chicken TLR1, TL...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825276/ https://www.ncbi.nlm.nih.gov/pubmed/23873159 http://dx.doi.org/10.1007/s13353-013-0161-1 |
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author | Sławińska, Anna D’Andrea, Mariasilvia Pilla, Fabio Bednarczyk, Marek Siwek, Maria |
author_facet | Sławińska, Anna D’Andrea, Mariasilvia Pilla, Fabio Bednarczyk, Marek Siwek, Maria |
author_sort | Sławińska, Anna |
collection | PubMed |
description | Toll-like receptors (TLRs) are members of the cellular receptors that constitute a major component of the evolutionary conserved pattern recognition system (PRR). TLRs are expressed in a wide variety of tissues and cell types. In this study we compared the expression profiles of the chicken TLR1, TLR2 and TLR5 genes in a range of organs (lung, ovary, liver, thymus, duodenum, spleen and large intestine) in commercial Hy-Line (HL) and indigenous Green-legged Partridgelike (GP) chickens. The level of mRNA was determined with RT-qPCR using the TaqMan probes for target and reference (ACTB) genes. We determined that the tissue profiles differed with respect to each TLR and they were ranked as follows: spleen, lungs, large intestine (TLR1), large intestine, lungs, thymus/ovary (TLR2) and lungs, thymus, liver (TLR5). A differential expression between HL and GP chickens was determined for TLR1 and TLR5 genes in large intestine and thymus of HL (P < 0.05) and GP (P < 0.05) chickens. We conclude that the commercial chickens expressed higher levels of TLR1 mRNA in large intestine and TLR5 mRNA in thymus than indigenous chickens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13353-013-0161-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3825276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-38252762013-11-21 Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens Sławińska, Anna D’Andrea, Mariasilvia Pilla, Fabio Bednarczyk, Marek Siwek, Maria J Appl Genet Animal Genetics • Short Communication Toll-like receptors (TLRs) are members of the cellular receptors that constitute a major component of the evolutionary conserved pattern recognition system (PRR). TLRs are expressed in a wide variety of tissues and cell types. In this study we compared the expression profiles of the chicken TLR1, TLR2 and TLR5 genes in a range of organs (lung, ovary, liver, thymus, duodenum, spleen and large intestine) in commercial Hy-Line (HL) and indigenous Green-legged Partridgelike (GP) chickens. The level of mRNA was determined with RT-qPCR using the TaqMan probes for target and reference (ACTB) genes. We determined that the tissue profiles differed with respect to each TLR and they were ranked as follows: spleen, lungs, large intestine (TLR1), large intestine, lungs, thymus/ovary (TLR2) and lungs, thymus, liver (TLR5). A differential expression between HL and GP chickens was determined for TLR1 and TLR5 genes in large intestine and thymus of HL (P < 0.05) and GP (P < 0.05) chickens. We conclude that the commercial chickens expressed higher levels of TLR1 mRNA in large intestine and TLR5 mRNA in thymus than indigenous chickens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13353-013-0161-1) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2013-07-20 2013 /pmc/articles/PMC3825276/ /pubmed/23873159 http://dx.doi.org/10.1007/s13353-013-0161-1 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Animal Genetics • Short Communication Sławińska, Anna D’Andrea, Mariasilvia Pilla, Fabio Bednarczyk, Marek Siwek, Maria Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
title | Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
title_full | Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
title_fullStr | Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
title_full_unstemmed | Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
title_short | Expression profiles of Toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
title_sort | expression profiles of toll-like receptors 1, 2 and 5 in selected organs of commercial and indigenous chickens |
topic | Animal Genetics • Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825276/ https://www.ncbi.nlm.nih.gov/pubmed/23873159 http://dx.doi.org/10.1007/s13353-013-0161-1 |
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