Isolation of monomeric photosystem II that retains the subunit PsbS

Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in thei...

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Detalles Bibliográficos
Autores principales: Haniewicz, Patrycja, De Sanctis, Daniele, Büchel, Claudia, Schröder, Wolfgang P., Loi, Maria Cecilia, Kieselbach, Thomas, Bochtler, Matthias, Piano, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Regular Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825537/
https://www.ncbi.nlm.nih.gov/pubmed/23975205
http://dx.doi.org/10.1007/s11120-013-9914-2
Descripción
Sumario:Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A “milder” procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501–1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.

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