Isolation of monomeric photosystem II that retains the subunit PsbS

Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in thei...

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Autores principales: Haniewicz, Patrycja, De Sanctis, Daniele, Büchel, Claudia, Schröder, Wolfgang P., Loi, Maria Cecilia, Kieselbach, Thomas, Bochtler, Matthias, Piano, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Regular Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825537/
https://www.ncbi.nlm.nih.gov/pubmed/23975205
http://dx.doi.org/10.1007/s11120-013-9914-2
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author Haniewicz, Patrycja
De Sanctis, Daniele
Büchel, Claudia
Schröder, Wolfgang P.
Loi, Maria Cecilia
Kieselbach, Thomas
Bochtler, Matthias
Piano, Dario
author_facet Haniewicz, Patrycja
De Sanctis, Daniele
Büchel, Claudia
Schröder, Wolfgang P.
Loi, Maria Cecilia
Kieselbach, Thomas
Bochtler, Matthias
Piano, Dario
author_sort Haniewicz, Patrycja
collection PubMed
description Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A “milder” procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501–1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.
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spelling pubmed-38255372013-11-21 Isolation of monomeric photosystem II that retains the subunit PsbS Haniewicz, Patrycja De Sanctis, Daniele Büchel, Claudia Schröder, Wolfgang P. Loi, Maria Cecilia Kieselbach, Thomas Bochtler, Matthias Piano, Dario Photosynth Res Regular Paper Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A “milder” procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501–1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants. Springer Netherlands 2013-08-24 2013 /pmc/articles/PMC3825537/ /pubmed/23975205 http://dx.doi.org/10.1007/s11120-013-9914-2 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Regular Paper
Haniewicz, Patrycja
De Sanctis, Daniele
Büchel, Claudia
Schröder, Wolfgang P.
Loi, Maria Cecilia
Kieselbach, Thomas
Bochtler, Matthias
Piano, Dario
Isolation of monomeric photosystem II that retains the subunit PsbS
title Isolation of monomeric photosystem II that retains the subunit PsbS
title_full Isolation of monomeric photosystem II that retains the subunit PsbS
title_fullStr Isolation of monomeric photosystem II that retains the subunit PsbS
title_full_unstemmed Isolation of monomeric photosystem II that retains the subunit PsbS
title_short Isolation of monomeric photosystem II that retains the subunit PsbS
title_sort isolation of monomeric photosystem ii that retains the subunit psbs
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825537/
https://www.ncbi.nlm.nih.gov/pubmed/23975205
http://dx.doi.org/10.1007/s11120-013-9914-2
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