Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes

Several methods of cultivation of Leishmania parasites are available, and among them, biphasic media like Nicolle–Novy–MacNeal medium are considered to be superior for the initial isolation of the parasites and their in vivo infectivity. In the present work, the efficacy of sheep blood–LB agar base...

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Detalles Bibliográficos
Autor principal: Nasiri, Vahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825544/
https://www.ncbi.nlm.nih.gov/pubmed/23925445
http://dx.doi.org/10.1007/s00436-013-3560-7
Descripción
Sumario:Several methods of cultivation of Leishmania parasites are available, and among them, biphasic media like Nicolle–Novy–MacNeal medium are considered to be superior for the initial isolation of the parasites and their in vivo infectivity. In the present work, the efficacy of sheep blood–LB agar base medium (SLM) was evaluated for the cultivation of Leishmania major promastigotes. The SLM supports the proliferation of the parasites and can be used for routine isolation and cultivation of Leishmania parasites with acceptable in vivo infectivity for research purposes.

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