Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes

Several methods of cultivation of Leishmania parasites are available, and among them, biphasic media like Nicolle–Novy–MacNeal medium are considered to be superior for the initial isolation of the parasites and their in vivo infectivity. In the present work, the efficacy of sheep blood–LB agar base...

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Detalles Bibliográficos
Autor principal: Nasiri, Vahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825544/
https://www.ncbi.nlm.nih.gov/pubmed/23925445
http://dx.doi.org/10.1007/s00436-013-3560-7
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author Nasiri, Vahid
author_facet Nasiri, Vahid
author_sort Nasiri, Vahid
collection PubMed
description Several methods of cultivation of Leishmania parasites are available, and among them, biphasic media like Nicolle–Novy–MacNeal medium are considered to be superior for the initial isolation of the parasites and their in vivo infectivity. In the present work, the efficacy of sheep blood–LB agar base medium (SLM) was evaluated for the cultivation of Leishmania major promastigotes. The SLM supports the proliferation of the parasites and can be used for routine isolation and cultivation of Leishmania parasites with acceptable in vivo infectivity for research purposes.
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spelling pubmed-38255442013-11-21 Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes Nasiri, Vahid Parasitol Res Original Paper Several methods of cultivation of Leishmania parasites are available, and among them, biphasic media like Nicolle–Novy–MacNeal medium are considered to be superior for the initial isolation of the parasites and their in vivo infectivity. In the present work, the efficacy of sheep blood–LB agar base medium (SLM) was evaluated for the cultivation of Leishmania major promastigotes. The SLM supports the proliferation of the parasites and can be used for routine isolation and cultivation of Leishmania parasites with acceptable in vivo infectivity for research purposes. Springer Berlin Heidelberg 2013-08-08 2013 /pmc/articles/PMC3825544/ /pubmed/23925445 http://dx.doi.org/10.1007/s00436-013-3560-7 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Nasiri, Vahid
Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes
title Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes
title_full Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes
title_fullStr Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes
title_full_unstemmed Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes
title_short Sheep blood–LB agar base medium (SLM) as a simple and suitable medium for the cultivation of Leishmania major promastigotes
title_sort sheep blood–lb agar base medium (slm) as a simple and suitable medium for the cultivation of leishmania major promastigotes
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3825544/
https://www.ncbi.nlm.nih.gov/pubmed/23925445
http://dx.doi.org/10.1007/s00436-013-3560-7
work_keys_str_mv AT nasirivahid sheepbloodlbagarbasemediumslmasasimpleandsuitablemediumforthecultivationofleishmaniamajorpromastigotes

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