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A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system

Within the nervous system, intracellular Cl(−) and pH regulate fundamental processes including cell proliferation, metabolism, synaptic transmission, and network excitability. Cl(−) and pH are often co-regulated, and network activity results in the movement of both Cl(−) and H(+). Tools to accuratel...

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Autores principales: Raimondo, Joseph V., Joyce, Bradley, Kay, Louise, Schlagheck, Theresa, Newey, Sarah E., Srinivas, Shankar, Akerman, Colin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826072/
https://www.ncbi.nlm.nih.gov/pubmed/24312004
http://dx.doi.org/10.3389/fncel.2013.00202
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author Raimondo, Joseph V.
Joyce, Bradley
Kay, Louise
Schlagheck, Theresa
Newey, Sarah E.
Srinivas, Shankar
Akerman, Colin J.
author_facet Raimondo, Joseph V.
Joyce, Bradley
Kay, Louise
Schlagheck, Theresa
Newey, Sarah E.
Srinivas, Shankar
Akerman, Colin J.
author_sort Raimondo, Joseph V.
collection PubMed
description Within the nervous system, intracellular Cl(−) and pH regulate fundamental processes including cell proliferation, metabolism, synaptic transmission, and network excitability. Cl(−) and pH are often co-regulated, and network activity results in the movement of both Cl(−) and H(+). Tools to accurately measure these ions are crucial for understanding their role under physiological and pathological conditions. Although genetically-encoded Cl(−) and pH sensors have been described previously, these either lack ion specificity or are unsuitable for neuronal use. Here we present ClopHensorN—a new genetically-encoded ratiometric Cl(−) and pH sensor that is optimized for the nervous system. We demonstrate the ability of ClopHensorN to dissociate and simultaneously quantify Cl(−) and H(+) concentrations under a variety of conditions. In addition, we establish the sensor's utility by characterizing activity-dependent ion dynamics in hippocampal neurons.
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spelling pubmed-38260722013-12-05 A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system Raimondo, Joseph V. Joyce, Bradley Kay, Louise Schlagheck, Theresa Newey, Sarah E. Srinivas, Shankar Akerman, Colin J. Front Cell Neurosci Neuroscience Within the nervous system, intracellular Cl(−) and pH regulate fundamental processes including cell proliferation, metabolism, synaptic transmission, and network excitability. Cl(−) and pH are often co-regulated, and network activity results in the movement of both Cl(−) and H(+). Tools to accurately measure these ions are crucial for understanding their role under physiological and pathological conditions. Although genetically-encoded Cl(−) and pH sensors have been described previously, these either lack ion specificity or are unsuitable for neuronal use. Here we present ClopHensorN—a new genetically-encoded ratiometric Cl(−) and pH sensor that is optimized for the nervous system. We demonstrate the ability of ClopHensorN to dissociate and simultaneously quantify Cl(−) and H(+) concentrations under a variety of conditions. In addition, we establish the sensor's utility by characterizing activity-dependent ion dynamics in hippocampal neurons. Frontiers Media S.A. 2013-11-13 /pmc/articles/PMC3826072/ /pubmed/24312004 http://dx.doi.org/10.3389/fncel.2013.00202 Text en Copyright © 2013 Raimondo, Joyce, Kay, Schlagheck, Newey, Srinivas and Akerman. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Raimondo, Joseph V.
Joyce, Bradley
Kay, Louise
Schlagheck, Theresa
Newey, Sarah E.
Srinivas, Shankar
Akerman, Colin J.
A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
title A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
title_full A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
title_fullStr A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
title_full_unstemmed A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
title_short A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
title_sort genetically-encoded chloride and ph sensor for dissociating ion dynamics in the nervous system
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826072/
https://www.ncbi.nlm.nih.gov/pubmed/24312004
http://dx.doi.org/10.3389/fncel.2013.00202
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