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New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35

Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and...

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Autores principales: Pan, Hua-Qi, Zhang, Song-Ya, Wang, Nan, Li, Zhan-Lin, Hua, Hui-Ming, Hu, Jiang-Chun, Wang, Shu-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826141/
https://www.ncbi.nlm.nih.gov/pubmed/24132178
http://dx.doi.org/10.3390/md11103891
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author Pan, Hua-Qi
Zhang, Song-Ya
Wang, Nan
Li, Zhan-Lin
Hua, Hui-Ming
Hu, Jiang-Chun
Wang, Shu-Jin
author_facet Pan, Hua-Qi
Zhang, Song-Ya
Wang, Nan
Li, Zhan-Lin
Hua, Hui-Ming
Hu, Jiang-Chun
Wang, Shu-Jin
author_sort Pan, Hua-Qi
collection PubMed
description Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and octadecylsilyl (ODS) and semi-preparative HPLC, led to the isolation and purification of five metabolites from the fermentation culture of 12A35. Two new spirotetronate antibiotics, lobophorins H (1) and I (2), along with three known analogues, O-β-kijanosyl-(1→17)-kijanolide (3), lobophorins B (4) and F (5) were characterized by 1D, 2D-NMR and MS data. These compounds exhibited significant inhibitory activities against Bacillus subtilis. Compounds 1 and 5 exhibited moderate activities against Staphylococcus aureus. In particular, the new compound lobophorin H (1) showed similar antibacterial activities against B. subtilis CMCC63501 to ampicillin.
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spelling pubmed-38261412013-11-13 New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 Pan, Hua-Qi Zhang, Song-Ya Wang, Nan Li, Zhan-Lin Hua, Hui-Ming Hu, Jiang-Chun Wang, Shu-Jin Mar Drugs Article Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and octadecylsilyl (ODS) and semi-preparative HPLC, led to the isolation and purification of five metabolites from the fermentation culture of 12A35. Two new spirotetronate antibiotics, lobophorins H (1) and I (2), along with three known analogues, O-β-kijanosyl-(1→17)-kijanolide (3), lobophorins B (4) and F (5) were characterized by 1D, 2D-NMR and MS data. These compounds exhibited significant inhibitory activities against Bacillus subtilis. Compounds 1 and 5 exhibited moderate activities against Staphylococcus aureus. In particular, the new compound lobophorin H (1) showed similar antibacterial activities against B. subtilis CMCC63501 to ampicillin. MDPI 2013-10-15 /pmc/articles/PMC3826141/ /pubmed/24132178 http://dx.doi.org/10.3390/md11103891 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pan, Hua-Qi
Zhang, Song-Ya
Wang, Nan
Li, Zhan-Lin
Hua, Hui-Ming
Hu, Jiang-Chun
Wang, Shu-Jin
New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
title New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
title_full New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
title_fullStr New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
title_full_unstemmed New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
title_short New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
title_sort new spirotetronate antibiotics, lobophorins h and i, from a south china sea-derived streptomyces sp. 12a35
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826141/
https://www.ncbi.nlm.nih.gov/pubmed/24132178
http://dx.doi.org/10.3390/md11103891
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