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Evaluation of Reference Genes and Normalization Strategy for Quantitative Real-Time PCR in Human Pancreatic Carcinoma
Histologically verified pairs (n = 10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C(q) values correlated with the degree of RNA degradation. This correlation wa...
Autores principales: | Mohelnikova-Duchonova, Beatrice, Oliverius, Martin, Honsova, Eva, Soucek, Pavel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826910/ https://www.ncbi.nlm.nih.gov/pubmed/22377737 http://dx.doi.org/10.3233/DMA-2011-0875 |
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