Cargando…
Smyd1b is required for skeletal and cardiac muscle function in zebrafish
Smyd1b is a member of the Smyd family that is specifically expressed in skeletal and cardiac muscles. Smyd1b plays a key role in thick filament assembly during myofibrillogenesis in skeletal muscles of zebrafish embryos. To better characterize Smyd1b function and its mechanism of action in myofibril...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826989/ https://www.ncbi.nlm.nih.gov/pubmed/24068325 http://dx.doi.org/10.1091/mbc.E13-06-0352 |
_version_ | 1782290987152834560 |
---|---|
author | Li, Huiqing Zhong, Yongwang Wang, Zengfeng Gao, Jie Xu, Jin Chu, Wuying Zhang, Jianshe Fang, Shenyun Du, Shao Jun |
author_facet | Li, Huiqing Zhong, Yongwang Wang, Zengfeng Gao, Jie Xu, Jin Chu, Wuying Zhang, Jianshe Fang, Shenyun Du, Shao Jun |
author_sort | Li, Huiqing |
collection | PubMed |
description | Smyd1b is a member of the Smyd family that is specifically expressed in skeletal and cardiac muscles. Smyd1b plays a key role in thick filament assembly during myofibrillogenesis in skeletal muscles of zebrafish embryos. To better characterize Smyd1b function and its mechanism of action in myofibrillogenesis, we analyzed the effects of smyd1b knockdown on myofibrillogenesis in skeletal and cardiac muscles of zebrafish embryos. The results show that knockdown of smyd1b causes significant disruption of myofibril organization in both skeletal and cardiac muscles of zebrafish embryos. Microarray and quantitative reverse transcription-PCR analyses show that knockdown of smyd1b up-regulates heat shock protein 90 (hsp90) and unc45b gene expression. Biochemical analysis reveals that Smyd1b can be coimmunoprecipitated with heat shock protein 90 α-1 and Unc45b, two myosin chaperones expressed in muscle cells. Consistent with its potential function in myosin folding and assembly, knockdown of smyd1b significantly reduces myosin protein accumulation without affecting mRNA expression. This likely results from increased myosin degradation involving unc45b overexpression. Together these data support the idea that Smyd1b may work together with myosin chaperones to control myosin folding, degradation, and assembly into sarcomeres during myofibrillogenesis. |
format | Online Article Text |
id | pubmed-3826989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-38269892014-01-30 Smyd1b is required for skeletal and cardiac muscle function in zebrafish Li, Huiqing Zhong, Yongwang Wang, Zengfeng Gao, Jie Xu, Jin Chu, Wuying Zhang, Jianshe Fang, Shenyun Du, Shao Jun Mol Biol Cell Articles Smyd1b is a member of the Smyd family that is specifically expressed in skeletal and cardiac muscles. Smyd1b plays a key role in thick filament assembly during myofibrillogenesis in skeletal muscles of zebrafish embryos. To better characterize Smyd1b function and its mechanism of action in myofibrillogenesis, we analyzed the effects of smyd1b knockdown on myofibrillogenesis in skeletal and cardiac muscles of zebrafish embryos. The results show that knockdown of smyd1b causes significant disruption of myofibril organization in both skeletal and cardiac muscles of zebrafish embryos. Microarray and quantitative reverse transcription-PCR analyses show that knockdown of smyd1b up-regulates heat shock protein 90 (hsp90) and unc45b gene expression. Biochemical analysis reveals that Smyd1b can be coimmunoprecipitated with heat shock protein 90 α-1 and Unc45b, two myosin chaperones expressed in muscle cells. Consistent with its potential function in myosin folding and assembly, knockdown of smyd1b significantly reduces myosin protein accumulation without affecting mRNA expression. This likely results from increased myosin degradation involving unc45b overexpression. Together these data support the idea that Smyd1b may work together with myosin chaperones to control myosin folding, degradation, and assembly into sarcomeres during myofibrillogenesis. The American Society for Cell Biology 2013-11-15 /pmc/articles/PMC3826989/ /pubmed/24068325 http://dx.doi.org/10.1091/mbc.E13-06-0352 Text en © 2013 Li et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Li, Huiqing Zhong, Yongwang Wang, Zengfeng Gao, Jie Xu, Jin Chu, Wuying Zhang, Jianshe Fang, Shenyun Du, Shao Jun Smyd1b is required for skeletal and cardiac muscle function in zebrafish |
title | Smyd1b is required for skeletal and cardiac muscle function in zebrafish |
title_full | Smyd1b is required for skeletal and cardiac muscle function in zebrafish |
title_fullStr | Smyd1b is required for skeletal and cardiac muscle function in zebrafish |
title_full_unstemmed | Smyd1b is required for skeletal and cardiac muscle function in zebrafish |
title_short | Smyd1b is required for skeletal and cardiac muscle function in zebrafish |
title_sort | smyd1b is required for skeletal and cardiac muscle function in zebrafish |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826989/ https://www.ncbi.nlm.nih.gov/pubmed/24068325 http://dx.doi.org/10.1091/mbc.E13-06-0352 |
work_keys_str_mv | AT lihuiqing smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT zhongyongwang smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT wangzengfeng smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT gaojie smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT xujin smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT chuwuying smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT zhangjianshe smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT fangshenyun smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish AT dushaojun smyd1bisrequiredforskeletalandcardiacmusclefunctioninzebrafish |