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Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum

A reverse genetics approach was used to investigate the role of γ-aminobutyric acid metabolism in the wheat pathogenic fungus Stagonospora nodorum. The creation of mutants lacking Sdh1, the gene encoding succinic semialdehyde dehydrogenase, resulted in strains that grew poorly on γ-aminobutyric acid...

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Autores principales: Mead, Oliver, Thynne, Eli, Winterberg, Britta, Solomon, Peter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827059/
https://www.ncbi.nlm.nih.gov/pubmed/24265684
http://dx.doi.org/10.1371/journal.pone.0078368
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author Mead, Oliver
Thynne, Eli
Winterberg, Britta
Solomon, Peter S.
author_facet Mead, Oliver
Thynne, Eli
Winterberg, Britta
Solomon, Peter S.
author_sort Mead, Oliver
collection PubMed
description A reverse genetics approach was used to investigate the role of γ-aminobutyric acid metabolism in the wheat pathogenic fungus Stagonospora nodorum. The creation of mutants lacking Sdh1, the gene encoding succinic semialdehyde dehydrogenase, resulted in strains that grew poorly on γ-aminobutyric acid as a nitrogen source. The sdh1 mutants were more susceptible to reactive oxygen stress but were less affected by increased growth temperatures. Pathogenicity assays revealed that the metabolism of γ-aminobutyric acid is required for complete pathogenicity. Growth assays of the wild-type and mutant strains showed that the inclusion of γ-aminobutyric acid as a supplement in minimal media (i.e., not as a nitrogen or carbon source) resulted in restricted growth but increased sporulation. The addition of glutamate, the precursor to GABA, had no effect on either growth or sporulation. The γ-aminobutyric acid effect on sporulation was found to be dose dependent and not restricted to Stagonospora nodorum with a similar effect observed in the dothideomycete Botryosphaeria sp. The positive effect on sporulation was assayed using isomers of γ-aminobutyric acid and other metabolites known to influence asexual development in Stagonospora nodorum but no effect was observed. These data demonstrate that γ-aminobutyric acid plays an important role in Stagonospora nodorum in responding to environmental stresses while also having a positive effect on asexual development.
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spelling pubmed-38270592013-11-21 Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum Mead, Oliver Thynne, Eli Winterberg, Britta Solomon, Peter S. PLoS One Research Article A reverse genetics approach was used to investigate the role of γ-aminobutyric acid metabolism in the wheat pathogenic fungus Stagonospora nodorum. The creation of mutants lacking Sdh1, the gene encoding succinic semialdehyde dehydrogenase, resulted in strains that grew poorly on γ-aminobutyric acid as a nitrogen source. The sdh1 mutants were more susceptible to reactive oxygen stress but were less affected by increased growth temperatures. Pathogenicity assays revealed that the metabolism of γ-aminobutyric acid is required for complete pathogenicity. Growth assays of the wild-type and mutant strains showed that the inclusion of γ-aminobutyric acid as a supplement in minimal media (i.e., not as a nitrogen or carbon source) resulted in restricted growth but increased sporulation. The addition of glutamate, the precursor to GABA, had no effect on either growth or sporulation. The γ-aminobutyric acid effect on sporulation was found to be dose dependent and not restricted to Stagonospora nodorum with a similar effect observed in the dothideomycete Botryosphaeria sp. The positive effect on sporulation was assayed using isomers of γ-aminobutyric acid and other metabolites known to influence asexual development in Stagonospora nodorum but no effect was observed. These data demonstrate that γ-aminobutyric acid plays an important role in Stagonospora nodorum in responding to environmental stresses while also having a positive effect on asexual development. Public Library of Science 2013-11-12 /pmc/articles/PMC3827059/ /pubmed/24265684 http://dx.doi.org/10.1371/journal.pone.0078368 Text en © 2013 Mead et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mead, Oliver
Thynne, Eli
Winterberg, Britta
Solomon, Peter S.
Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum
title Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum
title_full Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum
title_fullStr Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum
title_full_unstemmed Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum
title_short Characterising the Role of GABA and Its Metabolism in the Wheat Pathogen Stagonospora nodorum
title_sort characterising the role of gaba and its metabolism in the wheat pathogen stagonospora nodorum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827059/
https://www.ncbi.nlm.nih.gov/pubmed/24265684
http://dx.doi.org/10.1371/journal.pone.0078368
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