Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression

The objective of the current study was to determine the clinical significance of junctional adhesion molecule A (JAM-A) in patients with non-small cell lung cancer (NSCLC) and the biological function of JAM-A in NSCLC cell lines. We showed that JAM-A is predominantly expressed in cell membranes and...

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Autores principales: Zhang, Min, Luo, Wenting, Huang, Bo, Liu, Zihui, Sun, Limei, Zhang, Qingfu, Qiu, Xueshan, Xu, Ke, Wang, Enhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827132/
https://www.ncbi.nlm.nih.gov/pubmed/24265754
http://dx.doi.org/10.1371/journal.pone.0079173
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author Zhang, Min
Luo, Wenting
Huang, Bo
Liu, Zihui
Sun, Limei
Zhang, Qingfu
Qiu, Xueshan
Xu, Ke
Wang, Enhua
author_facet Zhang, Min
Luo, Wenting
Huang, Bo
Liu, Zihui
Sun, Limei
Zhang, Qingfu
Qiu, Xueshan
Xu, Ke
Wang, Enhua
author_sort Zhang, Min
collection PubMed
description The objective of the current study was to determine the clinical significance of junctional adhesion molecule A (JAM-A) in patients with non-small cell lung cancer (NSCLC) and the biological function of JAM-A in NSCLC cell lines. We showed that JAM-A is predominantly expressed in cell membranes and high expression of JAM-A occurred in 37% of lung tumor specimens compared to corresponding normal tissues. High expression of JAM-A was significantly correlated with TNM stage (P = 0.021), lymph node metastasis (P = 0.007), and decreased overall survival (P = 0.02), In addition, we observed that silencing JAM-A by small interfering RNA inhibited tumor cell proliferation and induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that knockdown of JAM-A decreased the protein levels of cyclin D1, CDK4, 6, and P-Rb. Thus, JAM-A plays an important role in NSCLC progression.
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spelling pubmed-38271322013-11-21 Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression Zhang, Min Luo, Wenting Huang, Bo Liu, Zihui Sun, Limei Zhang, Qingfu Qiu, Xueshan Xu, Ke Wang, Enhua PLoS One Research Article The objective of the current study was to determine the clinical significance of junctional adhesion molecule A (JAM-A) in patients with non-small cell lung cancer (NSCLC) and the biological function of JAM-A in NSCLC cell lines. We showed that JAM-A is predominantly expressed in cell membranes and high expression of JAM-A occurred in 37% of lung tumor specimens compared to corresponding normal tissues. High expression of JAM-A was significantly correlated with TNM stage (P = 0.021), lymph node metastasis (P = 0.007), and decreased overall survival (P = 0.02), In addition, we observed that silencing JAM-A by small interfering RNA inhibited tumor cell proliferation and induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that knockdown of JAM-A decreased the protein levels of cyclin D1, CDK4, 6, and P-Rb. Thus, JAM-A plays an important role in NSCLC progression. Public Library of Science 2013-11-12 /pmc/articles/PMC3827132/ /pubmed/24265754 http://dx.doi.org/10.1371/journal.pone.0079173 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Min
Luo, Wenting
Huang, Bo
Liu, Zihui
Sun, Limei
Zhang, Qingfu
Qiu, Xueshan
Xu, Ke
Wang, Enhua
Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression
title Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression
title_full Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression
title_fullStr Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression
title_full_unstemmed Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression
title_short Overexpression of JAM-A in Non-Small Cell Lung Cancer Correlates with Tumor Progression
title_sort overexpression of jam-a in non-small cell lung cancer correlates with tumor progression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827132/
https://www.ncbi.nlm.nih.gov/pubmed/24265754
http://dx.doi.org/10.1371/journal.pone.0079173
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