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A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite

Previously we developed genomic resources for orchids, including transcriptomic analyses using next-generation sequencing techniques and construction of a web-based orchid genomic database. Here, we report a modified molecular model of flower development in the Orchidaceae based on functional analys...

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Autores principales: Su, Chun-lin, Chen, Wan-Chieh, Lee, Ann-Ying, Chen, Chun-Yi, Chang, Yao-Chien Alex, Chao, Ya-Ting, Shih, Ming-Che
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827201/
https://www.ncbi.nlm.nih.gov/pubmed/24265826
http://dx.doi.org/10.1371/journal.pone.0080462
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author Su, Chun-lin
Chen, Wan-Chieh
Lee, Ann-Ying
Chen, Chun-Yi
Chang, Yao-Chien Alex
Chao, Ya-Ting
Shih, Ming-Che
author_facet Su, Chun-lin
Chen, Wan-Chieh
Lee, Ann-Ying
Chen, Chun-Yi
Chang, Yao-Chien Alex
Chao, Ya-Ting
Shih, Ming-Che
author_sort Su, Chun-lin
collection PubMed
description Previously we developed genomic resources for orchids, including transcriptomic analyses using next-generation sequencing techniques and construction of a web-based orchid genomic database. Here, we report a modified molecular model of flower development in the Orchidaceae based on functional analysis of gene expression profiles in Phalaenopsis aphrodite (a moth orchid) that revealed novel roles for the transcription factors involved in floral organ pattern formation. Phalaenopsis orchid floral organ-specific genes were identified by microarray analysis. Several critical transcription factors including AP3, PI, AP1 and AGL6, displayed distinct spatial distribution patterns. Phylogenetic analysis of orchid MADS box genes was conducted to infer the evolutionary relationship among floral organ-specific genes. The results suggest that gene duplication MADS box genes in orchid may have resulted in their gaining novel functions during evolution. Based on these analyses, a modified model of orchid flowering was proposed. Comparison of the expression profiles of flowers of a peloric mutant and wild-type Phalaenopsis orchid further identified genes associated with lip morphology and peloric effects. Large scale investigation of gene expression profiles revealed that homeotic genes from the ABCDE model of flower development classes A and B in the Phalaenopsis orchid have novel functions due to evolutionary diversification, and display differential expression patterns.
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spelling pubmed-38272012013-11-21 A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite Su, Chun-lin Chen, Wan-Chieh Lee, Ann-Ying Chen, Chun-Yi Chang, Yao-Chien Alex Chao, Ya-Ting Shih, Ming-Che PLoS One Research Article Previously we developed genomic resources for orchids, including transcriptomic analyses using next-generation sequencing techniques and construction of a web-based orchid genomic database. Here, we report a modified molecular model of flower development in the Orchidaceae based on functional analysis of gene expression profiles in Phalaenopsis aphrodite (a moth orchid) that revealed novel roles for the transcription factors involved in floral organ pattern formation. Phalaenopsis orchid floral organ-specific genes were identified by microarray analysis. Several critical transcription factors including AP3, PI, AP1 and AGL6, displayed distinct spatial distribution patterns. Phylogenetic analysis of orchid MADS box genes was conducted to infer the evolutionary relationship among floral organ-specific genes. The results suggest that gene duplication MADS box genes in orchid may have resulted in their gaining novel functions during evolution. Based on these analyses, a modified model of orchid flowering was proposed. Comparison of the expression profiles of flowers of a peloric mutant and wild-type Phalaenopsis orchid further identified genes associated with lip morphology and peloric effects. Large scale investigation of gene expression profiles revealed that homeotic genes from the ABCDE model of flower development classes A and B in the Phalaenopsis orchid have novel functions due to evolutionary diversification, and display differential expression patterns. Public Library of Science 2013-11-12 /pmc/articles/PMC3827201/ /pubmed/24265826 http://dx.doi.org/10.1371/journal.pone.0080462 Text en © 2013 Su et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Su, Chun-lin
Chen, Wan-Chieh
Lee, Ann-Ying
Chen, Chun-Yi
Chang, Yao-Chien Alex
Chao, Ya-Ting
Shih, Ming-Che
A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite
title A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite
title_full A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite
title_fullStr A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite
title_full_unstemmed A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite
title_short A Modified ABCDE Model of Flowering in Orchids Based on Gene Expression Profiling Studies of the Moth Orchid Phalaenopsis aphrodite
title_sort modified abcde model of flowering in orchids based on gene expression profiling studies of the moth orchid phalaenopsis aphrodite
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827201/
https://www.ncbi.nlm.nih.gov/pubmed/24265826
http://dx.doi.org/10.1371/journal.pone.0080462
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