Cargando…

A Simple Alkaline Method for Decellularizing Human Amniotic Membrane for Cell Culture

Human amniotic membrane is a standard substratum used to culture limbal epithelial stem cells for transplantation to patients with limbal stem cell deficiency. Various methods were developed to decellularize amniotic membrane, because denuded membrane is poorly immunogenic and better supports repopu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Saghizadeh, Mehrnoosh, Winkler, Michael A., Kramerov, Andrei A., Hemmati, David M., Ghiam, Chantelle A., Dimitrijevich, Slobodan D., Sareen, Dhruv, Ornelas, Loren, Ghiasi, Homayon, Brunken, William J., Maguen, Ezra, Rabinowitz, Yaron S., Svendsen, Clive N., Jirsova, Katerina, Ljubimov, Alexander V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827346/ https://www.ncbi.nlm.nih.gov/pubmed/24236148 http://dx.doi.org/10.1371/journal.pone.0079632

Ejemplares similares

Persistence of reduced expression of putative stem cell markers and slow wound healing in cultured diabetic limbal epithelial cells
por: Kramerov, Andrei A., et al.
Publicado: (2015)

Concise Review: Stem Cells for Corneal Wound Healing
por: Saghizadeh, Mehrnoosh, et al.
Publicado: (2017)

Differentially Expressed Wound Healing-Related microRNAs in the Human Diabetic Cornea
por: Funari, Vincent A., et al.
Publicado: (2013)

Reversal of dual epigenetic repression of non-canonical Wnt-5a normalises diabetic corneal epithelial wound healing and stem cells
por: Shah, Ruchi, et al.
Publicado: (2023)

Alterations of epithelial stem cell marker patterns in human diabetic corneas and effects of c-met gene therapy
por: Saghizadeh, Mehrnoosh, et al.
Publicado: (2011)

Targeting miR-146a to Treat Delayed Wound Healing in Human Diabetic Organ-Cultured Corneas
por: Winkler, Michael A., et al.
Publicado: (2014)

Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells
por: Leszczynska, Aleksandra, et al.
Publicado: (2018)

Integrated Transcriptome and Proteome Analyses Reveal the Regulatory Role of miR-146a in Human Limbal Epithelium via Notch Signaling
por: Poe, Adam J., et al.
Publicado: (2020)

Different tropism of adenoviruses and adeno-associated viruses to corneal cells: implications for corneal gene therapy
por: Liu, J., et al.
Publicado: (2008)

Inhibition of Apoptosis Blocks Human Motor Neuron Cell Death in a Stem Cell Model of Spinal Muscular Atrophy
por: Sareen, Dhruv, et al.
Publicado: (2012)

Role of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein K (gK) Pathogenic CD8(+) T Cells in Exacerbation of Eye Disease
por: Jaggi, Ujjaldeep, et al.
Publicado: (2018)

MicroRNA and Protein Cargos of Human Limbal Epithelial Cell-Derived Exosomes and Their Regulatory Roles in Limbal Stromal Cells of Diabetic and Non-Diabetic Corneas
por: Verma, Nagendra, et al.
Publicado: (2023)

Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
por: Yucer, Nur, et al.
Publicado: (2017)

Presence and distribution of 14-3-3 proteins in human ocular surface tissues
por: Shankardas, Jwalitha, et al.
Publicado: (2008)

Genome-wide analysis suggests a differential microRNA signature associated with normal and diabetic human corneal limbus
por: Kulkarni, Mangesh, et al.
Publicado: (2017)

NPR-B natriuretic peptide receptors in human corneal epithelium: mRNA, immunohistochemistochemical, protein, and biochemical pharmacology studies
por: Katoli, Parvaneh, et al.
Publicado: (2010)

Immunosuppressive Functions of M2 Macrophages Derived from iPSCs of Patients with ALS and Healthy Controls
por: Zhao, Weihua, et al.
Publicado: (2020)

Gene expression changes in the retina following subretinal injection of human neural progenitor cells into a rodent model for retinal degeneration
por: Jones, Melissa K., et al.
Publicado: (2016)

Characterization and functional expression of the natriuretic peptide system in human lens epithelial cells
por: Cammarata, Patrick R., et al.
Publicado: (2010)

ZBED4, a cone and Müller cell protein in human retina, has a different cellular expression in mouse
por: Saghizadeh, Mehrnoosh, et al.
Publicado: (2011)

GMP-grade human neural progenitors delivered subretinally protect vision in rat model of retinal degeneration and survive in minipigs
por: Lu, Bin, et al.
Publicado: (2023)

Knockout of signal peptide peptidase in the eye reduces HSV-1 replication and eye disease in ocularly infected mice
por: Wang, Shaohui, et al.
Publicado: (2022)

SARS-CoV-2 and its beta variant of concern infect human conjunctival epithelial cells and induce differential antiviral innate immune response
por: Singh, Sneha, et al.
Publicado: (2022)

Phytoplasma Infection could Affect Chemical Composition of Artemisia sieberi
por: Hemmati, Chamran, et al.
Publicado: (2019)

Effects of two different decellularization routes on the mechanical properties of decellularized lungs
por: Urbano, Jessica Julioti, et al.
Publicado: (2017)

Decellularized Porcine Cartilage Scaffold; Validation of Decellularization and Evaluation of Biomarkers of Chondrogenesis
por: Stone, Roxanne N., et al.
Publicado: (2021)

Can Heart Valve Decellularization Be Standardized? A Review of the Parameters Used for the Quality Control of Decellularization Processes
por: Naso, F., et al.
Publicado: (2022)

CCR2(+) migratory macrophages with M1 status are the early-responders in the cornea of HSV-1 infected mice
por: Lee, Dhong Hyun, et al.
Publicado: (2019)

Herpes Simplex Virus 1 Glycoproteins Differentially Regulate the Activity of Costimulatory Molecules and T Cells
por: Matundan, Harry H., et al.
Publicado: (2022)

Cortical neurons derived from human pluripotent stem cells lacking FMRP display altered spontaneous firing patterns
por: Das Sharma, Shreya, et al.
Publicado: (2020)

Optimized protocol for whole organ decellularization
por: Schmitt, A., et al.
Publicado: (2017)

Comparison of Decellularization Protocols for Preparing a Decellularized Porcine Annulus Fibrosus Scaffold
por: Xu, Haiwei, et al.
Publicado: (2014)

Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications
por: Nouri Barkestani, Mahsa, et al.
Publicado: (2021)

Decellularization of placentas: establishing a protocol
por: Leonel, L.C.P.C., et al.
Publicado: (2017)

Involvement of STAT4 in IgG subtype switching and ocular HSV-1 replication in mice
por: Allen, Sariah J., et al.
Publicado: (2010)

Ocular infection of mice with an avirulent recombinant HSV-1 expressing IL-4 and an attenuated HSV-1 strain generates virulent recombinants in vivo
por: Mott, Kevin R., et al.
Publicado: (2010)

A comparative study of experimental mouse models of central nervous system demyelination
por: Dumitrascu, Oana M., et al.
Publicado: (2014)

Blocking HSV-1 glycoprotein K binding to signal peptide peptidase reduces virus infectivity in vitro and in vivo
por: Wang, Shaohui, et al.
Publicado: (2021)

Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods
por: SESLİ, Melis, et al.
Publicado: (2018)

Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization
por: Isidan, Abdulkadir, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_9gs4bk6ep6vcj8mphac37ur9kg