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Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin

Enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. E. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. Using bacteriophage as an alternative treatment to drug-resistant bacteria has been revit...

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Autores principales: Zhang, Wenhui, Mi, Zhiqiang, Yin, Xiuyun, Fan, Hang, An, Xiaoping, Zhang, Zhiyi, Chen, Jiankui, Tong, Yigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827423/
https://www.ncbi.nlm.nih.gov/pubmed/24236180
http://dx.doi.org/10.1371/journal.pone.0080435
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author Zhang, Wenhui
Mi, Zhiqiang
Yin, Xiuyun
Fan, Hang
An, Xiaoping
Zhang, Zhiyi
Chen, Jiankui
Tong, Yigang
author_facet Zhang, Wenhui
Mi, Zhiqiang
Yin, Xiuyun
Fan, Hang
An, Xiaoping
Zhang, Zhiyi
Chen, Jiankui
Tong, Yigang
author_sort Zhang, Wenhui
collection PubMed
description Enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. E. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. Using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. In this research, an E. faecalis bacteriophage named IME-EF1 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that the isolated IME-EF1 belong to the Siphoviridae family, and has a linear double-stranded DNA genome consisting of 57,081 nucleotides. The IME-EF1 genome has a 40.04% G+C content and contains 98 putative coding sequences. In addition, IME-EF1 has an isometric head with a width of 35 nm to 60 nm and length of 75 nm to 90 nm, as well as morphology resembling a tadpole. IME-EF1 can adsorb to its host cells within 9 min, with an absorbance rate more than 99% and a latent period time of 25 min. The endolysin of IME-EF1 contains a CHAP domain in its N-terminal and has a wider bactericidal spectrum than its parental bacteriophage, including 2 strains of vancomycin-resistant E. faecalis. When administrated intraperitoneally, one dose of IME-EF1 or its endolysin can reduce bacterial count in the blood and protected the mice from a lethal challenge of E. faecalis, with a survival rate of 60% or 80%, respectively. Although bacteriophage could rescue mice from bacterial challenge, to the best of our knowledge, this study further supports the potential function of bacteriophage in dealing with E. faecalis infection in vivo. The results also indicated that the newly isolated bacteriophage IME-EF1 enriched the arsenal library of lytic E. faecalis bacteriophages and presented another choice for phage therapy in the future.
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spelling pubmed-38274232013-11-14 Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin Zhang, Wenhui Mi, Zhiqiang Yin, Xiuyun Fan, Hang An, Xiaoping Zhang, Zhiyi Chen, Jiankui Tong, Yigang PLoS One Research Article Enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. E. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. Using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. In this research, an E. faecalis bacteriophage named IME-EF1 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that the isolated IME-EF1 belong to the Siphoviridae family, and has a linear double-stranded DNA genome consisting of 57,081 nucleotides. The IME-EF1 genome has a 40.04% G+C content and contains 98 putative coding sequences. In addition, IME-EF1 has an isometric head with a width of 35 nm to 60 nm and length of 75 nm to 90 nm, as well as morphology resembling a tadpole. IME-EF1 can adsorb to its host cells within 9 min, with an absorbance rate more than 99% and a latent period time of 25 min. The endolysin of IME-EF1 contains a CHAP domain in its N-terminal and has a wider bactericidal spectrum than its parental bacteriophage, including 2 strains of vancomycin-resistant E. faecalis. When administrated intraperitoneally, one dose of IME-EF1 or its endolysin can reduce bacterial count in the blood and protected the mice from a lethal challenge of E. faecalis, with a survival rate of 60% or 80%, respectively. Although bacteriophage could rescue mice from bacterial challenge, to the best of our knowledge, this study further supports the potential function of bacteriophage in dealing with E. faecalis infection in vivo. The results also indicated that the newly isolated bacteriophage IME-EF1 enriched the arsenal library of lytic E. faecalis bacteriophages and presented another choice for phage therapy in the future. Public Library of Science 2013-11-13 /pmc/articles/PMC3827423/ /pubmed/24236180 http://dx.doi.org/10.1371/journal.pone.0080435 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Wenhui
Mi, Zhiqiang
Yin, Xiuyun
Fan, Hang
An, Xiaoping
Zhang, Zhiyi
Chen, Jiankui
Tong, Yigang
Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
title Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
title_full Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
title_fullStr Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
title_full_unstemmed Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
title_short Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
title_sort characterization of enterococcus faecalis phage ime-ef1 and its endolysin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827423/
https://www.ncbi.nlm.nih.gov/pubmed/24236180
http://dx.doi.org/10.1371/journal.pone.0080435
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