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K-RAS and P53 Mutations in Association with COX-2 and Htert Expression and Clinico-Pathological Status of Nsclc Patients

We evaluated the occurrence of mutations in P53, K-RAS, COX-2, expression of COX-2 and hTERT and relations among clinicopathological signs. P53 mutations were identified in 34.4% of tumours, the majority of them occurring in SCC (squamous cell carcinoma, 55.6%). K-RAS was mutated in 12.2% of NSCLC t...

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Detalles Bibliográficos
Autores principales: Stražišar, Mojca, Rott, Tomaž, Glavač, Damjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3827803/
https://www.ncbi.nlm.nih.gov/pubmed/18957720
http://dx.doi.org/10.1155/2008/232743
Descripción
Sumario:We evaluated the occurrence of mutations in P53, K-RAS, COX-2, expression of COX-2 and hTERT and relations among clinicopathological signs. P53 mutations were identified in 34.4% of tumours, the majority of them occurring in SCC (squamous cell carcinoma, 55.6%). K-RAS was mutated in 12.2% of NSCLC tumours, the majority of the mutations being found in ADC (adenocarcinoma, 27.0%). Mutational screening detected three different COX-2 mutations and five different P53 mutations, published for the first time. With RT-PCR we observed that the expression of the tested genes, hTERT and COX-2, was highly significant for ADC (p < 0.01) and SCC (p < 0.05). Statistical analysis of the combined results revealed significant correlation between expression of COX-2 and hTERT (p < 0.001), hTERT expression and staging (p < 0.05) and survival (p < 0.01). A positive correlation between COX-2 expression and K-RAS mutation (p <0.05) was also observed. This study provides insight into associations between the analysed biomarkers and the clinical-pathological data of the patients.