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Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers
Incoming Simian Virus 40 particles bind to their cellular receptor, the glycolipid GM1, in the plasma membrane and thereby induce membrane deformation beneath the virion leading to endocytosis and infection. Efficient membrane deformation depends on receptor lipid structure and the organization of b...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828148/ https://www.ncbi.nlm.nih.gov/pubmed/24244125 http://dx.doi.org/10.1371/journal.pcbi.1003310 |
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author | Szklarczyk, Oliwia M. González-Segredo, Nélido Kukura, Philipp Oppenheim, Ariella Choquet, Daniel Sandoghdar, Vahid Helenius, Ari Sbalzarini, Ivo F. Ewers, Helge |
author_facet | Szklarczyk, Oliwia M. González-Segredo, Nélido Kukura, Philipp Oppenheim, Ariella Choquet, Daniel Sandoghdar, Vahid Helenius, Ari Sbalzarini, Ivo F. Ewers, Helge |
author_sort | Szklarczyk, Oliwia M. |
collection | PubMed |
description | Incoming Simian Virus 40 particles bind to their cellular receptor, the glycolipid GM1, in the plasma membrane and thereby induce membrane deformation beneath the virion leading to endocytosis and infection. Efficient membrane deformation depends on receptor lipid structure and the organization of binding sites on the internalizing particle. To determine the role of receptor diffusion, concentration and the number of receptors required for stable binding in this interaction, we analyze the binding of SV40 to GM1 in supported membrane bilayers by computational modeling based on experimental data. We measure the diffusion rates of SV40 virions in solution by fluorescence correlation spectroscopy and of the receptor in bilayers by single molecule tracking. Quartz-crystal microbalance with dissipation (QCM-D) is used to measure binding of SV40 virus-like particles to bilayers containing the viral receptor GM1. We develop a phenomenological stochastic dynamics model calibrated against this data, and use it to investigate the early events of virus attachment to lipid membranes. Our results indicate that SV40 requires at least 4 attached receptors to achieve stable binding. We moreover find that receptor diffusion is essential for the establishment of stable binding over the physiological range of receptor concentrations and that receptor concentration controls the mode of viral motion on the target membrane. Our results provide quantitative insight into the initial events of virus-host interaction at the nanoscopic level. |
format | Online Article Text |
id | pubmed-3828148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38281482013-11-16 Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers Szklarczyk, Oliwia M. González-Segredo, Nélido Kukura, Philipp Oppenheim, Ariella Choquet, Daniel Sandoghdar, Vahid Helenius, Ari Sbalzarini, Ivo F. Ewers, Helge PLoS Comput Biol Research Article Incoming Simian Virus 40 particles bind to their cellular receptor, the glycolipid GM1, in the plasma membrane and thereby induce membrane deformation beneath the virion leading to endocytosis and infection. Efficient membrane deformation depends on receptor lipid structure and the organization of binding sites on the internalizing particle. To determine the role of receptor diffusion, concentration and the number of receptors required for stable binding in this interaction, we analyze the binding of SV40 to GM1 in supported membrane bilayers by computational modeling based on experimental data. We measure the diffusion rates of SV40 virions in solution by fluorescence correlation spectroscopy and of the receptor in bilayers by single molecule tracking. Quartz-crystal microbalance with dissipation (QCM-D) is used to measure binding of SV40 virus-like particles to bilayers containing the viral receptor GM1. We develop a phenomenological stochastic dynamics model calibrated against this data, and use it to investigate the early events of virus attachment to lipid membranes. Our results indicate that SV40 requires at least 4 attached receptors to achieve stable binding. We moreover find that receptor diffusion is essential for the establishment of stable binding over the physiological range of receptor concentrations and that receptor concentration controls the mode of viral motion on the target membrane. Our results provide quantitative insight into the initial events of virus-host interaction at the nanoscopic level. Public Library of Science 2013-11-14 /pmc/articles/PMC3828148/ /pubmed/24244125 http://dx.doi.org/10.1371/journal.pcbi.1003310 Text en © 2013 Szklarczyk et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Szklarczyk, Oliwia M. González-Segredo, Nélido Kukura, Philipp Oppenheim, Ariella Choquet, Daniel Sandoghdar, Vahid Helenius, Ari Sbalzarini, Ivo F. Ewers, Helge Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers |
title | Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers |
title_full | Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers |
title_fullStr | Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers |
title_full_unstemmed | Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers |
title_short | Receptor Concentration and Diffusivity Control Multivalent Binding of Sv40 to Membrane Bilayers |
title_sort | receptor concentration and diffusivity control multivalent binding of sv40 to membrane bilayers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828148/ https://www.ncbi.nlm.nih.gov/pubmed/24244125 http://dx.doi.org/10.1371/journal.pcbi.1003310 |
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