Cargando…

High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects

Early vascularization is a prerequisite for successful bone healing and endothelial progenitor cells (EPC), seeded on appropriate biomaterials, can improve vascularization. The type of biomaterial influences EPC function with bioglass evoking a vascularizing response. In this study the influence of...

Descripción completa

Detalles Bibliográficos
Autores principales: Eldesoqi, Karam, Seebach, Caroline, Nguyen Ngoc, Christina, Meier, Simon, Nau, Christoph, Schaible, Alexander, Marzi, Ingo, Henrich, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828289/
https://www.ncbi.nlm.nih.gov/pubmed/24244419
http://dx.doi.org/10.1371/journal.pone.0079058
_version_ 1782291218014666752
author Eldesoqi, Karam
Seebach, Caroline
Nguyen Ngoc, Christina
Meier, Simon
Nau, Christoph
Schaible, Alexander
Marzi, Ingo
Henrich, Dirk
author_facet Eldesoqi, Karam
Seebach, Caroline
Nguyen Ngoc, Christina
Meier, Simon
Nau, Christoph
Schaible, Alexander
Marzi, Ingo
Henrich, Dirk
author_sort Eldesoqi, Karam
collection PubMed
description Early vascularization is a prerequisite for successful bone healing and endothelial progenitor cells (EPC), seeded on appropriate biomaterials, can improve vascularization. The type of biomaterial influences EPC function with bioglass evoking a vascularizing response. In this study the influence of a composite biomaterial based on polylactic acid (PLA) and either 20 or 40% bioglass, BG20 and BG40, respectively, on the differentiation and survival of EPCs in vitro was investigated. Subsequently, the effect of the composite material on early vascularization in a rat calvarial critical size defect model with or without EPCs was evaluated. Human EPCs were cultured with β-TCP, PLA, BG20 or BG40, and seeding efficacy, cell viability, cell morphology and apoptosis were analysed in vitro. BG40 released the most calcium, and improved endothelial differentiation and vitality best. This effect was mimicked by adding an equivalent amount of calcium to the medium and was diminished in the presence of the calcium chelator, EGTA. To analyze the effect of BG40 and EPCs in vivo, a 6-mm diameter critical size calvarial defect was created in rats (n = 12). Controls (n = 6) received BG40 and the treatment group (n = 6) received BG40 seeded with 5×10(5) rat EPCs. Vascularization after 1 week was significantly improved when EPCs were seeded onto BG40, compared to implanting BG40 alone. This indicates that Ca(2+) release improves EPC differentiation and is useful for enhanced early vascularization in critical size bone defects.
format Online
Article
Text
id pubmed-3828289
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38282892013-11-16 High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects Eldesoqi, Karam Seebach, Caroline Nguyen Ngoc, Christina Meier, Simon Nau, Christoph Schaible, Alexander Marzi, Ingo Henrich, Dirk PLoS One Research Article Early vascularization is a prerequisite for successful bone healing and endothelial progenitor cells (EPC), seeded on appropriate biomaterials, can improve vascularization. The type of biomaterial influences EPC function with bioglass evoking a vascularizing response. In this study the influence of a composite biomaterial based on polylactic acid (PLA) and either 20 or 40% bioglass, BG20 and BG40, respectively, on the differentiation and survival of EPCs in vitro was investigated. Subsequently, the effect of the composite material on early vascularization in a rat calvarial critical size defect model with or without EPCs was evaluated. Human EPCs were cultured with β-TCP, PLA, BG20 or BG40, and seeding efficacy, cell viability, cell morphology and apoptosis were analysed in vitro. BG40 released the most calcium, and improved endothelial differentiation and vitality best. This effect was mimicked by adding an equivalent amount of calcium to the medium and was diminished in the presence of the calcium chelator, EGTA. To analyze the effect of BG40 and EPCs in vivo, a 6-mm diameter critical size calvarial defect was created in rats (n = 12). Controls (n = 6) received BG40 and the treatment group (n = 6) received BG40 seeded with 5×10(5) rat EPCs. Vascularization after 1 week was significantly improved when EPCs were seeded onto BG40, compared to implanting BG40 alone. This indicates that Ca(2+) release improves EPC differentiation and is useful for enhanced early vascularization in critical size bone defects. Public Library of Science 2013-11-14 /pmc/articles/PMC3828289/ /pubmed/24244419 http://dx.doi.org/10.1371/journal.pone.0079058 Text en © 2013 Eldesoqi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Eldesoqi, Karam
Seebach, Caroline
Nguyen Ngoc, Christina
Meier, Simon
Nau, Christoph
Schaible, Alexander
Marzi, Ingo
Henrich, Dirk
High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects
title High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects
title_full High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects
title_fullStr High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects
title_full_unstemmed High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects
title_short High Calcium Bioglass Enhances Differentiation and Survival of Endothelial Progenitor Cells, Inducing Early Vascularization in Critical Size Bone Defects
title_sort high calcium bioglass enhances differentiation and survival of endothelial progenitor cells, inducing early vascularization in critical size bone defects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828289/
https://www.ncbi.nlm.nih.gov/pubmed/24244419
http://dx.doi.org/10.1371/journal.pone.0079058
work_keys_str_mv AT eldesoqikaram highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT seebachcaroline highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT nguyenngocchristina highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT meiersimon highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT nauchristoph highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT schaiblealexander highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT marziingo highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects
AT henrichdirk highcalciumbioglassenhancesdifferentiationandsurvivalofendothelialprogenitorcellsinducingearlyvascularizationincriticalsizebonedefects