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Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology
DNA aptamers were developed against murine norovirus (MNV) using SELEX (Systematic Evolution of Ligands by EXponential enrichment). Nine rounds of SELEX led to the discovery of AG3, a promising aptamer with very high affinity for MNV as well as for lab-synthesized capsids of a common human norovirus...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828344/ https://www.ncbi.nlm.nih.gov/pubmed/24244426 http://dx.doi.org/10.1371/journal.pone.0079087 |
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author | Giamberardino, Amanda Labib, Mahmoud Hassan, Eman M. Tetro, Jason A. Springthorpe, Susan Sattar, Syed A. Berezovski, Maxim V. DeRosa, Maria C. |
author_facet | Giamberardino, Amanda Labib, Mahmoud Hassan, Eman M. Tetro, Jason A. Springthorpe, Susan Sattar, Syed A. Berezovski, Maxim V. DeRosa, Maria C. |
author_sort | Giamberardino, Amanda |
collection | PubMed |
description | DNA aptamers were developed against murine norovirus (MNV) using SELEX (Systematic Evolution of Ligands by EXponential enrichment). Nine rounds of SELEX led to the discovery of AG3, a promising aptamer with very high affinity for MNV as well as for lab-synthesized capsids of a common human norovirus (HuNoV) outbreak strain (GII.3). Using fluorescence anisotropy, AG3 was found to bind with MNV with affinity in the low picomolar range. The aptamer could cross-react with HuNoV though it was selected against MNV. As compared to a non-specific DNA control sequence, the norovirus-binding affinity of AG3 was about a million-fold higher. In further tests, the aptamer also showed nearly a million-fold higher affinity for the noroviruses than for the feline calicivirus (FCV), a virus similar in size and structure to noroviruses. AG3 was incorporated into a simple electrochemical sensor using a gold nanoparticle-modified screen-printed carbon electrode (GNPs-SPCE). The aptasensor could detect MNV with a limit of detection of approximately 180 virus particles, for possible on-site applications. The lead aptamer candidate and the aptasensor platform show promise for the rapid detection and identification of noroviruses in environmental and clinical samples. |
format | Online Article Text |
id | pubmed-3828344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38283442013-11-16 Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology Giamberardino, Amanda Labib, Mahmoud Hassan, Eman M. Tetro, Jason A. Springthorpe, Susan Sattar, Syed A. Berezovski, Maxim V. DeRosa, Maria C. PLoS One Research Article DNA aptamers were developed against murine norovirus (MNV) using SELEX (Systematic Evolution of Ligands by EXponential enrichment). Nine rounds of SELEX led to the discovery of AG3, a promising aptamer with very high affinity for MNV as well as for lab-synthesized capsids of a common human norovirus (HuNoV) outbreak strain (GII.3). Using fluorescence anisotropy, AG3 was found to bind with MNV with affinity in the low picomolar range. The aptamer could cross-react with HuNoV though it was selected against MNV. As compared to a non-specific DNA control sequence, the norovirus-binding affinity of AG3 was about a million-fold higher. In further tests, the aptamer also showed nearly a million-fold higher affinity for the noroviruses than for the feline calicivirus (FCV), a virus similar in size and structure to noroviruses. AG3 was incorporated into a simple electrochemical sensor using a gold nanoparticle-modified screen-printed carbon electrode (GNPs-SPCE). The aptasensor could detect MNV with a limit of detection of approximately 180 virus particles, for possible on-site applications. The lead aptamer candidate and the aptasensor platform show promise for the rapid detection and identification of noroviruses in environmental and clinical samples. Public Library of Science 2013-11-14 /pmc/articles/PMC3828344/ /pubmed/24244426 http://dx.doi.org/10.1371/journal.pone.0079087 Text en © 2013 Giamberardino et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Giamberardino, Amanda Labib, Mahmoud Hassan, Eman M. Tetro, Jason A. Springthorpe, Susan Sattar, Syed A. Berezovski, Maxim V. DeRosa, Maria C. Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology |
title | Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology |
title_full | Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology |
title_fullStr | Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology |
title_full_unstemmed | Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology |
title_short | Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology |
title_sort | ultrasensitive norovirus detection using dna aptasensor technology |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828344/ https://www.ncbi.nlm.nih.gov/pubmed/24244426 http://dx.doi.org/10.1371/journal.pone.0079087 |
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