Cargando…

Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis

Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interact...

Descripción completa

Detalles Bibliográficos
Autores principales: Kwon, Hayeong, Jeong, Kyuho, Pak, Yunbae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828866/
https://www.ncbi.nlm.nih.gov/pubmed/19778377
http://dx.doi.org/10.1111/j.1582-4934.2008.00391.x
_version_ 1782291297615216640
author Kwon, Hayeong
Jeong, Kyuho
Pak, Yunbae
author_facet Kwon, Hayeong
Jeong, Kyuho
Pak, Yunbae
author_sort Kwon, Hayeong
collection PubMed
description Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2 with phospho-ERK was prevented when tyrosine 19 is mutated to alanine. Insulin relocalized phospho-ERK and pY19-caveolin-2 to the nucleus and their nuclear co-localization was impaired by U0126. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested the insulin-induced nuclear localization of ERK with no change in the insulin-stimulated ERK activation. Of consequence, the caveolin-2 siRNA attenuated the ERK-mediated c-Jun and cyclinD1 expression and DNA synthesis by insulin. In addition, actin cytoskeleton influenced the nuclear translocation of caveolin-2-ERK complex. Collectively, our findings underscore the importance of pY19-caveolin-2 with the spatial coordination by insulin in ERK-mediated mitogenic regulation of insulin signalling and indicate that the phosphorylation of pY19-caveolin-2 is required for actin cytoskeleton-dependent ERK nuclear import.
format Online
Article
Text
id pubmed-3828866
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-38288662015-04-27 Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis Kwon, Hayeong Jeong, Kyuho Pak, Yunbae J Cell Mol Med Articles Mitogenic regulation by caveolin-2 in response to insulin was investigated. Insulin triggered phosphorylation of caveolin-2 on tyrosine 19. Insulin increased the interaction between pY19-caveolin-2 and phospho-ERK, and that interaction was inhibited by a MEK inhibitor U0126. Insulin-induced interaction of caveolin-2 with phospho-ERK was prevented when tyrosine 19 is mutated to alanine. Insulin relocalized phospho-ERK and pY19-caveolin-2 to the nucleus and their nuclear co-localization was impaired by U0126. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested the insulin-induced nuclear localization of ERK with no change in the insulin-stimulated ERK activation. Of consequence, the caveolin-2 siRNA attenuated the ERK-mediated c-Jun and cyclinD1 expression and DNA synthesis by insulin. In addition, actin cytoskeleton influenced the nuclear translocation of caveolin-2-ERK complex. Collectively, our findings underscore the importance of pY19-caveolin-2 with the spatial coordination by insulin in ERK-mediated mitogenic regulation of insulin signalling and indicate that the phosphorylation of pY19-caveolin-2 is required for actin cytoskeleton-dependent ERK nuclear import. Blackwell Publishing Ltd 2009-08 2009-09-23 /pmc/articles/PMC3828866/ /pubmed/19778377 http://dx.doi.org/10.1111/j.1582-4934.2008.00391.x Text en © 2008 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Articles
Kwon, Hayeong
Jeong, Kyuho
Pak, Yunbae
Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
title Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
title_full Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
title_fullStr Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
title_full_unstemmed Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
title_short Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
title_sort identification of py19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828866/
https://www.ncbi.nlm.nih.gov/pubmed/19778377
http://dx.doi.org/10.1111/j.1582-4934.2008.00391.x
work_keys_str_mv AT kwonhayeong identificationofpy19caveolin2asapositiveregulatorofinsulinstimulatedactincytoskeletondependentmitogenesis
AT jeongkyuho identificationofpy19caveolin2asapositiveregulatorofinsulinstimulatedactincytoskeletondependentmitogenesis
AT pakyunbae identificationofpy19caveolin2asapositiveregulatorofinsulinstimulatedactincytoskeletondependentmitogenesis