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Functional characterization of the progestagen-associated endometrial protein gene in human melanoma

Utilizing gene microarray profiling of melanoma samples, we have recently identified a novel gene overexpressed in both thick primary and metastatic melanomas. This gene, progestagen-associated endometrial protein (PAEP), has never before been implicated in the oncogenic processes of melanoma, with...

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Autores principales: Ren, Suping, Liu, Suhu, Howell Jr, Paul M, Zhang, Guangyu, Pannell, Lewis, Samant, Rajeev, Shevde-Samant, Lalita, Tucker, J Allan, Fodstad, Oystein, Riker, Adam I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829010/
https://www.ncbi.nlm.nih.gov/pubmed/19799645
http://dx.doi.org/10.1111/j.1582-4934.2009.00922.x
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author Ren, Suping
Liu, Suhu
Howell Jr, Paul M
Zhang, Guangyu
Pannell, Lewis
Samant, Rajeev
Shevde-Samant, Lalita
Tucker, J Allan
Fodstad, Oystein
Riker, Adam I
author_facet Ren, Suping
Liu, Suhu
Howell Jr, Paul M
Zhang, Guangyu
Pannell, Lewis
Samant, Rajeev
Shevde-Samant, Lalita
Tucker, J Allan
Fodstad, Oystein
Riker, Adam I
author_sort Ren, Suping
collection PubMed
description Utilizing gene microarray profiling of melanoma samples, we have recently identified a novel gene overexpressed in both thick primary and metastatic melanomas. This gene, progestagen-associated endometrial protein (PAEP), has never before been implicated in the oncogenic processes of melanoma, with its true function in oncogenesis and tumour progression relatively unknown. Overexpression of the PAEP gene in freshly procured thick primary and metastatic melanoma samples (58%) and daughter cell lines (77%) is confirmed by quantitative RT-PCR, immunohistochemistry, Western blotting and mass spectrometric analysis. We suggest that PAEP gene overexpression is involved with melanoma tumour progression as well as an aggressive phenotype. Transfection of melanoma cells with PAEP small interfering RNA (siRNA) reveals a significant decrease in soft agar colony formation and a marked inhibition of both cell migration and cell invasion. Furthermore, we establish stable melanoma transfectants via PAEP lentiviral small hairpin RNA (shRNA), examine their growth characteristics in a murine xenograft model and reveal that tumour growth is significantly inhibited in two separate melanoma cell lines. Our data strongly implicate the PAEP gene as a tumour growth promoter with oncogenic properties and a potential therapeutic target for patients with advanced melanoma.
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spelling pubmed-38290102015-04-20 Functional characterization of the progestagen-associated endometrial protein gene in human melanoma Ren, Suping Liu, Suhu Howell Jr, Paul M Zhang, Guangyu Pannell, Lewis Samant, Rajeev Shevde-Samant, Lalita Tucker, J Allan Fodstad, Oystein Riker, Adam I J Cell Mol Med Original Articles Utilizing gene microarray profiling of melanoma samples, we have recently identified a novel gene overexpressed in both thick primary and metastatic melanomas. This gene, progestagen-associated endometrial protein (PAEP), has never before been implicated in the oncogenic processes of melanoma, with its true function in oncogenesis and tumour progression relatively unknown. Overexpression of the PAEP gene in freshly procured thick primary and metastatic melanoma samples (58%) and daughter cell lines (77%) is confirmed by quantitative RT-PCR, immunohistochemistry, Western blotting and mass spectrometric analysis. We suggest that PAEP gene overexpression is involved with melanoma tumour progression as well as an aggressive phenotype. Transfection of melanoma cells with PAEP small interfering RNA (siRNA) reveals a significant decrease in soft agar colony formation and a marked inhibition of both cell migration and cell invasion. Furthermore, we establish stable melanoma transfectants via PAEP lentiviral small hairpin RNA (shRNA), examine their growth characteristics in a murine xenograft model and reveal that tumour growth is significantly inhibited in two separate melanoma cell lines. Our data strongly implicate the PAEP gene as a tumour growth promoter with oncogenic properties and a potential therapeutic target for patients with advanced melanoma. Blackwell Publishing Ltd 2010-06 2009-10-03 /pmc/articles/PMC3829010/ /pubmed/19799645 http://dx.doi.org/10.1111/j.1582-4934.2009.00922.x Text en © 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Original Articles
Ren, Suping
Liu, Suhu
Howell Jr, Paul M
Zhang, Guangyu
Pannell, Lewis
Samant, Rajeev
Shevde-Samant, Lalita
Tucker, J Allan
Fodstad, Oystein
Riker, Adam I
Functional characterization of the progestagen-associated endometrial protein gene in human melanoma
title Functional characterization of the progestagen-associated endometrial protein gene in human melanoma
title_full Functional characterization of the progestagen-associated endometrial protein gene in human melanoma
title_fullStr Functional characterization of the progestagen-associated endometrial protein gene in human melanoma
title_full_unstemmed Functional characterization of the progestagen-associated endometrial protein gene in human melanoma
title_short Functional characterization of the progestagen-associated endometrial protein gene in human melanoma
title_sort functional characterization of the progestagen-associated endometrial protein gene in human melanoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829010/
https://www.ncbi.nlm.nih.gov/pubmed/19799645
http://dx.doi.org/10.1111/j.1582-4934.2009.00922.x
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