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Effect of whey protein on plasma amino acids in diabetic mice

The aim of this study was to investigate the effect of whey protein on plasma amino acid levels in a mouse model of type II diabetes, using high-performance liquid chromatography (HPLC). The composition and content of amino acids in the whey proteins were analyzed using HPLC. Type I and type II diab...

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Autores principales: HAN, TING, CAI, DONGLIAN, GENG, SHANSHAN, WANG, YING, ZHEN, HUI, WU, PEIYING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829761/
https://www.ncbi.nlm.nih.gov/pubmed/24255674
http://dx.doi.org/10.3892/etm.2013.1329
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author HAN, TING
CAI, DONGLIAN
GENG, SHANSHAN
WANG, YING
ZHEN, HUI
WU, PEIYING
author_facet HAN, TING
CAI, DONGLIAN
GENG, SHANSHAN
WANG, YING
ZHEN, HUI
WU, PEIYING
author_sort HAN, TING
collection PubMed
description The aim of this study was to investigate the effect of whey protein on plasma amino acid levels in a mouse model of type II diabetes, using high-performance liquid chromatography (HPLC). The composition and content of amino acids in the whey proteins were analyzed using HPLC. Type I and type II diabetic mouse models were prepared using streptozotocin (STZ) and normal mice were used as a control. The ICR mice in each group were then randomly divided into four subgroups, to which 0, 10, 20 and 40% whey protein, respectively, was administered for four weeks. Changes in the plasma amino acid levels were observed in each group. The proportions of leucine, isoleucine and valine in the whey proteins were 14.40, 5.93 and 5.32% of the total amino acids, respectively, that is, the branched-chain amino acid content was 25.65%. The levels of branched-chain amino acids increased in the plasma of the normal and model mice following the administration of whey proteins by gavage and the amino acid levels increased as the concentration of the administered protein increased. In addition, the branched-chain amino acid levels in the blood of the model mice were higher than those in the normal mice. The levels of plasma amino acids in diabetic mice increased following gavage with whey protein, which is rich in branched-chain amino acids.
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spelling pubmed-38297612013-11-19 Effect of whey protein on plasma amino acids in diabetic mice HAN, TING CAI, DONGLIAN GENG, SHANSHAN WANG, YING ZHEN, HUI WU, PEIYING Exp Ther Med Articles The aim of this study was to investigate the effect of whey protein on plasma amino acid levels in a mouse model of type II diabetes, using high-performance liquid chromatography (HPLC). The composition and content of amino acids in the whey proteins were analyzed using HPLC. Type I and type II diabetic mouse models were prepared using streptozotocin (STZ) and normal mice were used as a control. The ICR mice in each group were then randomly divided into four subgroups, to which 0, 10, 20 and 40% whey protein, respectively, was administered for four weeks. Changes in the plasma amino acid levels were observed in each group. The proportions of leucine, isoleucine and valine in the whey proteins were 14.40, 5.93 and 5.32% of the total amino acids, respectively, that is, the branched-chain amino acid content was 25.65%. The levels of branched-chain amino acids increased in the plasma of the normal and model mice following the administration of whey proteins by gavage and the amino acid levels increased as the concentration of the administered protein increased. In addition, the branched-chain amino acid levels in the blood of the model mice were higher than those in the normal mice. The levels of plasma amino acids in diabetic mice increased following gavage with whey protein, which is rich in branched-chain amino acids. D.A. Spandidos 2013-12 2013-10-07 /pmc/articles/PMC3829761/ /pubmed/24255674 http://dx.doi.org/10.3892/etm.2013.1329 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
HAN, TING
CAI, DONGLIAN
GENG, SHANSHAN
WANG, YING
ZHEN, HUI
WU, PEIYING
Effect of whey protein on plasma amino acids in diabetic mice
title Effect of whey protein on plasma amino acids in diabetic mice
title_full Effect of whey protein on plasma amino acids in diabetic mice
title_fullStr Effect of whey protein on plasma amino acids in diabetic mice
title_full_unstemmed Effect of whey protein on plasma amino acids in diabetic mice
title_short Effect of whey protein on plasma amino acids in diabetic mice
title_sort effect of whey protein on plasma amino acids in diabetic mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829761/
https://www.ncbi.nlm.nih.gov/pubmed/24255674
http://dx.doi.org/10.3892/etm.2013.1329
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