Cargando…
Bone formation induced by BMP-2 in human osteosarcoma cells
Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH ( br ) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
201
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829777/ https://www.ncbi.nlm.nih.gov/pubmed/23900689 http://dx.doi.org/ 10.3892/ijo.2013.2030 |
_version_ | 1782291387018903552 |
---|---|
author | WANG, LIN PARK, PAUL LA MARCA, FRANK THAN, KHOI RAHMAN, SHAYAN LIN, CHIA-YING |
author_facet | WANG, LIN PARK, PAUL LA MARCA, FRANK THAN, KHOI RAHMAN, SHAYAN LIN, CHIA-YING |
author_sort | WANG, LIN |
collection | PubMed |
description | Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH ( br ) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDH ( br ) and non-tumorigenic ALDH ( lo ) cells. qRT-PCR was used to quantify the gene expression. A xenograft model was used to verify the bone formation in vivo . There was significantly higher mRNA expression of BMPR1B and BMPR2 in ALDH ( lo ) cells compared with that in ALDH ( br ) cells and the BMPR1B expression in ALDH ( lo ) cells was ∼8-fold higher compared to that in ALDH ( br ) cells. BMP-2 was also found to induce higher transcription of osteogenic markers Runx-2, Osterix (Osx), alkaline phosphatase (ALP) and collagen type I in ALDH ( lo ) cells compared to ALDH ( br ) cells, which were mediated by the canonical Smad signaling pathway. In vivo , BMP-2 was identified to induce bone formation in both ALDH ( br ) and ALDH ( lo ) cells. All animals receiving 1×10 ( 4 ) ALDH ( lo ) cells treated with 30 μ g of BMP-2 per animal showed bone formation within 1–2 weeks after injection in mice. Bone formation induced by BMP-2 in ALDH ( lo ) cells showed significantly more bone mineral content compared to that in ALDH ( br ) cells. BMP-2 induces bone formation in heterogeneous osteosarcoma cells and BMP-2 may have a promising therapeutic role for treating human osteosarcoma by inducing differentiation along an osteogenic pathway. |
format | Online Article Text |
id | pubmed-3829777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate |
201 |
publisher |
D.A. Spandidos
|
record_format | MEDLINE/PubMed |
spelling | pubmed-38297772013-11-18 Bone formation induced by BMP-2 in human osteosarcoma cells WANG, LIN PARK, PAUL LA MARCA, FRANK THAN, KHOI RAHMAN, SHAYAN LIN, CHIA-YING Int J Oncol Articles Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH ( br ) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDH ( br ) and non-tumorigenic ALDH ( lo ) cells. qRT-PCR was used to quantify the gene expression. A xenograft model was used to verify the bone formation in vivo . There was significantly higher mRNA expression of BMPR1B and BMPR2 in ALDH ( lo ) cells compared with that in ALDH ( br ) cells and the BMPR1B expression in ALDH ( lo ) cells was ∼8-fold higher compared to that in ALDH ( br ) cells. BMP-2 was also found to induce higher transcription of osteogenic markers Runx-2, Osterix (Osx), alkaline phosphatase (ALP) and collagen type I in ALDH ( lo ) cells compared to ALDH ( br ) cells, which were mediated by the canonical Smad signaling pathway. In vivo , BMP-2 was identified to induce bone formation in both ALDH ( br ) and ALDH ( lo ) cells. All animals receiving 1×10 ( 4 ) ALDH ( lo ) cells treated with 30 μ g of BMP-2 per animal showed bone formation within 1–2 weeks after injection in mice. Bone formation induced by BMP-2 in ALDH ( lo ) cells showed significantly more bone mineral content compared to that in ALDH ( br ) cells. BMP-2 induces bone formation in heterogeneous osteosarcoma cells and BMP-2 may have a promising therapeutic role for treating human osteosarcoma by inducing differentiation along an osteogenic pathway. D.A. Spandidos 2013 -07- 23 /pmc/articles/PMC3829777/ /pubmed/23900689 http://dx.doi.org/ 10.3892/ijo.2013.2030 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles WANG, LIN PARK, PAUL LA MARCA, FRANK THAN, KHOI RAHMAN, SHAYAN LIN, CHIA-YING Bone formation induced by BMP-2 in human osteosarcoma cells |
title |
Bone formation induced by BMP-2 in human osteosarcoma cells
|
title_full |
Bone formation induced by BMP-2 in human osteosarcoma cells
|
title_fullStr |
Bone formation induced by BMP-2 in human osteosarcoma cells
|
title_full_unstemmed |
Bone formation induced by BMP-2 in human osteosarcoma cells
|
title_short |
Bone formation induced by BMP-2 in human osteosarcoma cells
|
title_sort | bone formation induced by bmp-2 in human osteosarcoma cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829777/ https://www.ncbi.nlm.nih.gov/pubmed/23900689 http://dx.doi.org/ 10.3892/ijo.2013.2030 |
work_keys_str_mv | AT wanglin boneformationinducedbybmp2inhumanosteosarcomacells AT parkpaul boneformationinducedbybmp2inhumanosteosarcomacells AT lamarcafrank boneformationinducedbybmp2inhumanosteosarcomacells AT thankhoi boneformationinducedbybmp2inhumanosteosarcomacells AT rahmanshayan boneformationinducedbybmp2inhumanosteosarcomacells AT linchiaying boneformationinducedbybmp2inhumanosteosarcomacells |