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Bone formation induced by BMP-2 in human osteosarcoma cells

Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH ( br ) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in...

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Autores principales: WANG, LIN, PARK, PAUL, LA MARCA, FRANK, THAN, KHOI, RAHMAN, SHAYAN, LIN, CHIA-YING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 201
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829777/
https://www.ncbi.nlm.nih.gov/pubmed/23900689
http://dx.doi.org/ 10.3892/ijo.2013.2030
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author WANG, LIN
PARK, PAUL
LA MARCA, FRANK
THAN, KHOI
RAHMAN, SHAYAN
LIN, CHIA-YING
author_facet WANG, LIN
PARK, PAUL
LA MARCA, FRANK
THAN, KHOI
RAHMAN, SHAYAN
LIN, CHIA-YING
author_sort WANG, LIN
collection PubMed
description Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH ( br ) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDH ( br ) and non-tumorigenic ALDH ( lo ) cells. qRT-PCR was used to quantify the gene expression. A xenograft model was used to verify the bone formation in vivo . There was significantly higher mRNA expression of BMPR1B and BMPR2 in ALDH ( lo ) cells compared with that in ALDH ( br ) cells and the BMPR1B expression in ALDH ( lo ) cells was ∼8-fold higher compared to that in ALDH ( br ) cells. BMP-2 was also found to induce higher transcription of osteogenic markers Runx-2, Osterix (Osx), alkaline phosphatase (ALP) and collagen type I in ALDH ( lo ) cells compared to ALDH ( br ) cells, which were mediated by the canonical Smad signaling pathway. In vivo , BMP-2 was identified to induce bone formation in both ALDH ( br ) and ALDH ( lo ) cells. All animals receiving 1×10 ( 4 ) ALDH ( lo ) cells treated with 30 μ g of BMP-2 per animal showed bone formation within 1–2 weeks after injection in mice. Bone formation induced by BMP-2 in ALDH ( lo ) cells showed significantly more bone mineral content compared to that in ALDH ( br ) cells. BMP-2 induces bone formation in heterogeneous osteosarcoma cells and BMP-2 may have a promising therapeutic role for treating human osteosarcoma by inducing differentiation along an osteogenic pathway.
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spelling pubmed-38297772013-11-18 Bone formation induced by BMP-2 in human osteosarcoma cells WANG, LIN PARK, PAUL LA MARCA, FRANK THAN, KHOI RAHMAN, SHAYAN LIN, CHIA-YING Int J Oncol Articles Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH ( br ) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDH ( br ) and non-tumorigenic ALDH ( lo ) cells. qRT-PCR was used to quantify the gene expression. A xenograft model was used to verify the bone formation in vivo . There was significantly higher mRNA expression of BMPR1B and BMPR2 in ALDH ( lo ) cells compared with that in ALDH ( br ) cells and the BMPR1B expression in ALDH ( lo ) cells was ∼8-fold higher compared to that in ALDH ( br ) cells. BMP-2 was also found to induce higher transcription of osteogenic markers Runx-2, Osterix (Osx), alkaline phosphatase (ALP) and collagen type I in ALDH ( lo ) cells compared to ALDH ( br ) cells, which were mediated by the canonical Smad signaling pathway. In vivo , BMP-2 was identified to induce bone formation in both ALDH ( br ) and ALDH ( lo ) cells. All animals receiving 1×10 ( 4 ) ALDH ( lo ) cells treated with 30 μ g of BMP-2 per animal showed bone formation within 1–2 weeks after injection in mice. Bone formation induced by BMP-2 in ALDH ( lo ) cells showed significantly more bone mineral content compared to that in ALDH ( br ) cells. BMP-2 induces bone formation in heterogeneous osteosarcoma cells and BMP-2 may have a promising therapeutic role for treating human osteosarcoma by inducing differentiation along an osteogenic pathway. D.A. Spandidos 2013 -07- 23 /pmc/articles/PMC3829777/ /pubmed/23900689 http://dx.doi.org/ 10.3892/ijo.2013.2030 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, LIN
PARK, PAUL
LA MARCA, FRANK
THAN, KHOI
RAHMAN, SHAYAN
LIN, CHIA-YING
Bone formation induced by BMP-2 in human osteosarcoma cells
title Bone formation induced by BMP-2 in human osteosarcoma cells
title_full Bone formation induced by BMP-2 in human osteosarcoma cells
title_fullStr Bone formation induced by BMP-2 in human osteosarcoma cells
title_full_unstemmed Bone formation induced by BMP-2 in human osteosarcoma cells
title_short Bone formation induced by BMP-2 in human osteosarcoma cells
title_sort bone formation induced by bmp-2 in human osteosarcoma cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829777/
https://www.ncbi.nlm.nih.gov/pubmed/23900689
http://dx.doi.org/ 10.3892/ijo.2013.2030
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