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In vitro inhibition of mumps virus by retinoids
BACKGROUND: Mumps virus (MuV) is a highly infectious paramyxovirus closely related to measles virus (MeV). Despite the availability of a mumps vaccine, outbreaks continue to occur and no treatment options are available. Vitamin A and other naturally occurring retinoids inhibit the replication of MeV...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829803/ https://www.ncbi.nlm.nih.gov/pubmed/24225020 http://dx.doi.org/10.1186/1743-422X-10-337 |
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author | Soye, Kaitlin J Trottier, Claire Di Lenardo, Thomas Z Restori, Katherine H Reichman, Lee Miller, Wilson H Ward, Brian J |
author_facet | Soye, Kaitlin J Trottier, Claire Di Lenardo, Thomas Z Restori, Katherine H Reichman, Lee Miller, Wilson H Ward, Brian J |
author_sort | Soye, Kaitlin J |
collection | PubMed |
description | BACKGROUND: Mumps virus (MuV) is a highly infectious paramyxovirus closely related to measles virus (MeV). Despite the availability of a mumps vaccine, outbreaks continue to occur and no treatment options are available. Vitamin A and other naturally occurring retinoids inhibit the replication of MeV in vitro. METHODS: Anti-viral effects of retinoids were observed in cell culture using the myelomonocytic U937, NB4/R4, and Huh7/7.5 cells. Observations of anti-viral effect were quantified using TCID50 analysis. Molecular properties of the antiviral effect were analysed using quantitative RT-PCR and western blot. RESULTS: The current work demonstrates that retinoids inhibit MuV in vitro due to up-regulation of type I interferon (IFN) and IFN stimulated genes. This effect is mediated by nuclear retinoid receptor signalling and RIG-I is required. The antiviral retinoid-induced state makes cells less permissive to viral replication from subsequent challenge with either MuV or MeV for less than 12 hours. CONCLUSIONS: These results demonstrate that retinoids inhibit MuV replication in uninfected bystander cells through a retinoid inducible gene I (RIG-I), retinoic acid receptor (RAR) and IFN dependent manner making them refractory to subsequent rounds of viral replication. These observations raise the possibility that pharmacological doses of retinoids might have clinical benefit in MuV infection. |
format | Online Article Text |
id | pubmed-3829803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38298032013-11-16 In vitro inhibition of mumps virus by retinoids Soye, Kaitlin J Trottier, Claire Di Lenardo, Thomas Z Restori, Katherine H Reichman, Lee Miller, Wilson H Ward, Brian J Virol J Research BACKGROUND: Mumps virus (MuV) is a highly infectious paramyxovirus closely related to measles virus (MeV). Despite the availability of a mumps vaccine, outbreaks continue to occur and no treatment options are available. Vitamin A and other naturally occurring retinoids inhibit the replication of MeV in vitro. METHODS: Anti-viral effects of retinoids were observed in cell culture using the myelomonocytic U937, NB4/R4, and Huh7/7.5 cells. Observations of anti-viral effect were quantified using TCID50 analysis. Molecular properties of the antiviral effect were analysed using quantitative RT-PCR and western blot. RESULTS: The current work demonstrates that retinoids inhibit MuV in vitro due to up-regulation of type I interferon (IFN) and IFN stimulated genes. This effect is mediated by nuclear retinoid receptor signalling and RIG-I is required. The antiviral retinoid-induced state makes cells less permissive to viral replication from subsequent challenge with either MuV or MeV for less than 12 hours. CONCLUSIONS: These results demonstrate that retinoids inhibit MuV replication in uninfected bystander cells through a retinoid inducible gene I (RIG-I), retinoic acid receptor (RAR) and IFN dependent manner making them refractory to subsequent rounds of viral replication. These observations raise the possibility that pharmacological doses of retinoids might have clinical benefit in MuV infection. BioMed Central 2013-11-14 /pmc/articles/PMC3829803/ /pubmed/24225020 http://dx.doi.org/10.1186/1743-422X-10-337 Text en Copyright © 2013 Soye et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Soye, Kaitlin J Trottier, Claire Di Lenardo, Thomas Z Restori, Katherine H Reichman, Lee Miller, Wilson H Ward, Brian J In vitro inhibition of mumps virus by retinoids |
title | In vitro inhibition of mumps virus by retinoids |
title_full | In vitro inhibition of mumps virus by retinoids |
title_fullStr | In vitro inhibition of mumps virus by retinoids |
title_full_unstemmed | In vitro inhibition of mumps virus by retinoids |
title_short | In vitro inhibition of mumps virus by retinoids |
title_sort | in vitro inhibition of mumps virus by retinoids |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829803/ https://www.ncbi.nlm.nih.gov/pubmed/24225020 http://dx.doi.org/10.1186/1743-422X-10-337 |
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