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The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes

Herpes Simplex Virus 1 (HSV-1) is a major pathogen that causes human neurological diseases, including herpes simplex encephalitis (HSE). Previous studies have shown that astrocytes are involved in HSV-1 systemic pathogenesis in the central nervous system (CNS), although the mechanism remains unclear...

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Autores principales: Yue, Lei, Guo, Sujie, Zhang, Ying, Liu, Longding, Wang, Qingling, Wang, Xi, Shen, Dong, Wang, Lichun, Sun, Le, Wang, Jingjing, Liao, Yun, Li, Qihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829861/
https://www.ncbi.nlm.nih.gov/pubmed/24260274
http://dx.doi.org/10.1371/journal.pone.0079648
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author Yue, Lei
Guo, Sujie
Zhang, Ying
Liu, Longding
Wang, Qingling
Wang, Xi
Shen, Dong
Wang, Lichun
Sun, Le
Wang, Jingjing
Liao, Yun
Li, Qihan
author_facet Yue, Lei
Guo, Sujie
Zhang, Ying
Liu, Longding
Wang, Qingling
Wang, Xi
Shen, Dong
Wang, Lichun
Sun, Le
Wang, Jingjing
Liao, Yun
Li, Qihan
author_sort Yue, Lei
collection PubMed
description Herpes Simplex Virus 1 (HSV-1) is a major pathogen that causes human neurological diseases, including herpes simplex encephalitis (HSE). Previous studies have shown that astrocytes are involved in HSV-1 systemic pathogenesis in the central nervous system (CNS), although the mechanism remains unclear. In this study, a high-throughput RNAi library screening method was used to analyze the effect of host phosphatase gene regulation on HSV-1 replication using Macaca mulatta primary astrocytes in an in vitro culture system. The results showed that the downregulation of five phosphatase genes (PNKP, SNAP23, PTPRU, LOC714621 and PPM1M) significantly inhibited HSV-1 infection, suggesting that these phosphatases were needed in HSV-1 replication in rhesus astrocytes. Although statistically significant, the effect of downregulation of these phosphatases on HSV-1 replication in a human astrocytoma cell line appears to be more limited. Our results suggest that the phosphatase genes in astrocytes may regulate the immunological and pathological reactions caused by HSV-1 CNS infection through the regulation of HSV-1 replication or of multiple signal transduction pathways.
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spelling pubmed-38298612013-11-20 The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes Yue, Lei Guo, Sujie Zhang, Ying Liu, Longding Wang, Qingling Wang, Xi Shen, Dong Wang, Lichun Sun, Le Wang, Jingjing Liao, Yun Li, Qihan PLoS One Research Article Herpes Simplex Virus 1 (HSV-1) is a major pathogen that causes human neurological diseases, including herpes simplex encephalitis (HSE). Previous studies have shown that astrocytes are involved in HSV-1 systemic pathogenesis in the central nervous system (CNS), although the mechanism remains unclear. In this study, a high-throughput RNAi library screening method was used to analyze the effect of host phosphatase gene regulation on HSV-1 replication using Macaca mulatta primary astrocytes in an in vitro culture system. The results showed that the downregulation of five phosphatase genes (PNKP, SNAP23, PTPRU, LOC714621 and PPM1M) significantly inhibited HSV-1 infection, suggesting that these phosphatases were needed in HSV-1 replication in rhesus astrocytes. Although statistically significant, the effect of downregulation of these phosphatases on HSV-1 replication in a human astrocytoma cell line appears to be more limited. Our results suggest that the phosphatase genes in astrocytes may regulate the immunological and pathological reactions caused by HSV-1 CNS infection through the regulation of HSV-1 replication or of multiple signal transduction pathways. Public Library of Science 2013-11-15 /pmc/articles/PMC3829861/ /pubmed/24260274 http://dx.doi.org/10.1371/journal.pone.0079648 Text en © 2013 Yue et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yue, Lei
Guo, Sujie
Zhang, Ying
Liu, Longding
Wang, Qingling
Wang, Xi
Shen, Dong
Wang, Lichun
Sun, Le
Wang, Jingjing
Liao, Yun
Li, Qihan
The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes
title The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes
title_full The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes
title_fullStr The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes
title_full_unstemmed The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes
title_short The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected Astrocytes
title_sort modulation of phosphatase expression impacts the proliferation efficiency of hsv-1 in infected astrocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829861/
https://www.ncbi.nlm.nih.gov/pubmed/24260274
http://dx.doi.org/10.1371/journal.pone.0079648
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