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Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast

In a “two-component system,” extracellular stimuli are transmitted by the transfer of a phosphoryl group from a sensor histidine kinase to a response regulator (RR), a mechanism referred to as phosphorelay. In the fission yeast Schizosaccharomyces pombe, peroxide stress signals are transmitted by ph...

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Detalles Bibliográficos
Autores principales: Morigasaki, Susumu, Shiozaki, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829895/
https://www.ncbi.nlm.nih.gov/pubmed/24255738
http://dx.doi.org/10.4161/cib.25020
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author Morigasaki, Susumu
Shiozaki, Kazuhiro
author_facet Morigasaki, Susumu
Shiozaki, Kazuhiro
author_sort Morigasaki, Susumu
collection PubMed
description In a “two-component system,” extracellular stimuli are transmitted by the transfer of a phosphoryl group from a sensor histidine kinase to a response regulator (RR), a mechanism referred to as phosphorelay. In the fission yeast Schizosaccharomyces pombe, peroxide stress signals are transmitted by phosphorelay to the Mcs4 RR, which activates the Spc1 MAP kinase (MAPK) cascade. We previously demonstrated that a glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) physically interacts with Mcs4 and promotes phosphorelay signaling to Mcs4. Independently of the phosphorelay mechanism, Mcs4 also plays a critical role in osmostress signaling, as a part of the stable ternary complex with the Wis4 and Win1 MAPK kinase kinases (MAPKKKs). Interestingly, GAPDH dissociates from Mcs4 upon osmostress, while oxidative stress promotes their association. The Mcs4 RR may serve as a switching hub that mediates activation of the Wis4-Win1 MAPKKK heteromer in response to different forms of stress.
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spelling pubmed-38298952013-11-19 Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast Morigasaki, Susumu Shiozaki, Kazuhiro Commun Integr Biol Short Communication In a “two-component system,” extracellular stimuli are transmitted by the transfer of a phosphoryl group from a sensor histidine kinase to a response regulator (RR), a mechanism referred to as phosphorelay. In the fission yeast Schizosaccharomyces pombe, peroxide stress signals are transmitted by phosphorelay to the Mcs4 RR, which activates the Spc1 MAP kinase (MAPK) cascade. We previously demonstrated that a glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) physically interacts with Mcs4 and promotes phosphorelay signaling to Mcs4. Independently of the phosphorelay mechanism, Mcs4 also plays a critical role in osmostress signaling, as a part of the stable ternary complex with the Wis4 and Win1 MAPK kinase kinases (MAPKKKs). Interestingly, GAPDH dissociates from Mcs4 upon osmostress, while oxidative stress promotes their association. The Mcs4 RR may serve as a switching hub that mediates activation of the Wis4-Win1 MAPKKK heteromer in response to different forms of stress. Landes Bioscience 2013-09-01 2013-05-20 /pmc/articles/PMC3829895/ /pubmed/24255738 http://dx.doi.org/10.4161/cib.25020 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Short Communication
Morigasaki, Susumu
Shiozaki, Kazuhiro
Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast
title Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast
title_full Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast
title_fullStr Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast
title_full_unstemmed Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast
title_short Phosphorelay-dependent and -independent regulation of MAPKKK by the Mcs4 response regulator in fission yeast
title_sort phosphorelay-dependent and -independent regulation of mapkkk by the mcs4 response regulator in fission yeast
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829895/
https://www.ncbi.nlm.nih.gov/pubmed/24255738
http://dx.doi.org/10.4161/cib.25020
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