Cargando…

Marker-Free Phenotyping of Tumor Cells by Fractal Analysis of Reflection Interference Contrast Microscopy Images

[Image: see text] Phenotyping of tumor cells by marker-free quantification is important for cancer diagnostics. For the first time, fractal analysis of reflection interference contrast microscopy images of single living cells was employed as a new method to distinguish between different nanoscopic m...

Descripción completa

Detalles Bibliográficos
Autores principales: Klein, Katharina, Maier, Timo, Hirschfeld-Warneken, Vera C., Spatz, Joachim P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3831548/
https://www.ncbi.nlm.nih.gov/pubmed/24079895
http://dx.doi.org/10.1021/nl4030402
Descripción
Sumario:[Image: see text] Phenotyping of tumor cells by marker-free quantification is important for cancer diagnostics. For the first time, fractal analysis of reflection interference contrast microscopy images of single living cells was employed as a new method to distinguish between different nanoscopic membrane features of tumor cells. Since tumor progression correlates with a higher degree of chaos within the cell, it can be quantified mathematically by fractality. Our results show a high accuracy in identifying malignant cells with a failure chance of 3%, which is far better than today’s applied methods.