Cargando…

Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus

Resistance nodulation cell division (RND)-type efflux transporters play the main role in intrinsic resistance to various antimicrobial agents in many gram-negative bacteria. Here, we estimated 12 RND-type efflux transporter genes in Vibrio parahaemolyticus. Because VmeAB has already been characteriz...

Descripción completa

Detalles Bibliográficos
Autores principales: Matsuo, Taira, Nakamura, Koji, Kodama, Toshio, Mikami, Taro, Hiyoshi, Hirotaka, Tsuchiya, Tomofusa, Ogawa, Wakano, Kuroda, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3831635/
https://www.ncbi.nlm.nih.gov/pubmed/23894076
http://dx.doi.org/10.1002/mbo3.100
_version_ 1782291600525754368
author Matsuo, Taira
Nakamura, Koji
Kodama, Toshio
Mikami, Taro
Hiyoshi, Hirotaka
Tsuchiya, Tomofusa
Ogawa, Wakano
Kuroda, Teruo
author_facet Matsuo, Taira
Nakamura, Koji
Kodama, Toshio
Mikami, Taro
Hiyoshi, Hirotaka
Tsuchiya, Tomofusa
Ogawa, Wakano
Kuroda, Teruo
author_sort Matsuo, Taira
collection PubMed
description Resistance nodulation cell division (RND)-type efflux transporters play the main role in intrinsic resistance to various antimicrobial agents in many gram-negative bacteria. Here, we estimated 12 RND-type efflux transporter genes in Vibrio parahaemolyticus. Because VmeAB has already been characterized, we cloned the other 11 RND-type efflux transporter genes and characterized them in Escherichia coli KAM33 cells, a drug hypersusceptible strain. KAM33 expressing either VmeCD, VmeEF, or VmeYZ showed increased minimum inhibitory concentrations (MICs) for several antimicrobial agents. Additional four RND-type transporters were functional as efflux pumps only when co-expressed with VpoC, an outer membrane component in V. parahaemolyticus. Furthermore, VmeCD, VmeEF, and VmeYZ co-expressed with VpoC exhibited a broader substrate specificity and conferred higher resistance than that with TolC of E. coli. Deletion mutants of these transporter genes were constructed in V. parahaemolyticus. TM32 (ΔvmeAB and ΔvmeCD) had significantly decreased MICs for many antimicrobial agents and the number of viable cells after exposure to deoxycholate were markedly reduced. Strains in which 12 operons were all disrupted had very low MICs and much lower fluid accumulation in rabbit ileal loops. These results indicate that resistance nodulation cell division-type efflux transporters contribute not only to intrinsic resistance but also to exerting the virulence of V. parahaemolyticus.
format Online
Article
Text
id pubmed-3831635
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-38316352013-11-29 Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus Matsuo, Taira Nakamura, Koji Kodama, Toshio Mikami, Taro Hiyoshi, Hirotaka Tsuchiya, Tomofusa Ogawa, Wakano Kuroda, Teruo Microbiologyopen Original Research Resistance nodulation cell division (RND)-type efflux transporters play the main role in intrinsic resistance to various antimicrobial agents in many gram-negative bacteria. Here, we estimated 12 RND-type efflux transporter genes in Vibrio parahaemolyticus. Because VmeAB has already been characterized, we cloned the other 11 RND-type efflux transporter genes and characterized them in Escherichia coli KAM33 cells, a drug hypersusceptible strain. KAM33 expressing either VmeCD, VmeEF, or VmeYZ showed increased minimum inhibitory concentrations (MICs) for several antimicrobial agents. Additional four RND-type transporters were functional as efflux pumps only when co-expressed with VpoC, an outer membrane component in V. parahaemolyticus. Furthermore, VmeCD, VmeEF, and VmeYZ co-expressed with VpoC exhibited a broader substrate specificity and conferred higher resistance than that with TolC of E. coli. Deletion mutants of these transporter genes were constructed in V. parahaemolyticus. TM32 (ΔvmeAB and ΔvmeCD) had significantly decreased MICs for many antimicrobial agents and the number of viable cells after exposure to deoxycholate were markedly reduced. Strains in which 12 operons were all disrupted had very low MICs and much lower fluid accumulation in rabbit ileal loops. These results indicate that resistance nodulation cell division-type efflux transporters contribute not only to intrinsic resistance but also to exerting the virulence of V. parahaemolyticus. Blackwell Publishing Ltd 2013-10 2013-07-27 /pmc/articles/PMC3831635/ /pubmed/23894076 http://dx.doi.org/10.1002/mbo3.100 Text en © 2013 Published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Research
Matsuo, Taira
Nakamura, Koji
Kodama, Toshio
Mikami, Taro
Hiyoshi, Hirotaka
Tsuchiya, Tomofusa
Ogawa, Wakano
Kuroda, Teruo
Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus
title Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus
title_full Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus
title_fullStr Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus
title_full_unstemmed Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus
title_short Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus
title_sort characterization of all rnd-type multidrug efflux transporters in vibrio parahaemolyticus
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3831635/
https://www.ncbi.nlm.nih.gov/pubmed/23894076
http://dx.doi.org/10.1002/mbo3.100
work_keys_str_mv AT matsuotaira characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT nakamurakoji characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT kodamatoshio characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT mikamitaro characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT hiyoshihirotaka characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT tsuchiyatomofusa characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT ogawawakano characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus
AT kurodateruo characterizationofallrndtypemultidrugeffluxtransportersinvibrioparahaemolyticus