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Umbelliprenin Induces Apoptosis in CLL Cell Lines
Chronic lymphocytic leukemia (CLL) remains an incurable disease that requires innovative new approaches to improve therapeutic outcome. Many Ferula species, including F. asa-foetida, synthesize terpenyloxy coumarins. One of these coumarins is umbelliprenin, which has been implicated with induction o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832171/ https://www.ncbi.nlm.nih.gov/pubmed/24250490 |
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author | Ziai, Seyed Ali Gholami, Omid Iranshahi, Mehrdad Zamani, Amir Hassan Jeddi-Tehrani, Mahmood |
author_facet | Ziai, Seyed Ali Gholami, Omid Iranshahi, Mehrdad Zamani, Amir Hassan Jeddi-Tehrani, Mahmood |
author_sort | Ziai, Seyed Ali |
collection | PubMed |
description | Chronic lymphocytic leukemia (CLL) remains an incurable disease that requires innovative new approaches to improve therapeutic outcome. Many Ferula species, including F. asa-foetida, synthesize terpenyloxy coumarins. One of these coumarins is umbelliprenin, which has been implicated with induction of apoptosis in some cancer cell lines. In this study induction of apoptosis by umbelliprenin on Jurkat T-CLL and Raji B-CLL cell lines was studied. In this regard, cells were incubated with various concentrations of umbelliprenin in-vitro for different times and assayed for apoptosis with annexin V–FITC/PI double staining flowcytometry method. Results showed that umbelliprenin induced apoptosis in leukemic cells in a dose- and time-dependent manner and that CLL cells were more susceptible to umbelliprenin induced cell death than normal peripheral blood mononuclear cell (PBMCs). Moreover, we study the induction of apoptosis in Jurkat cells by umbelliprenin in the presence of interleukin 4 (IL-4) as an agent that causes resistance to apoptosis in CLL cells, was also student. We showed that IL-4 can not reduce apoptotic effect of umbelliprenin. The preferential toxicity of umbelliprenin for CLL cells, supports the hypothesis that oral administration of umbelliprenin in the form of foods or folk medicines containing this coumarin, might enhance protection against the development of CLL in man with little side effects. In conclusion, umbelliprenin may be an effective therapeutic agent in the treatment of CLL, and thus clinical studies with umbelliprenin may be appropriate. |
format | Online Article Text |
id | pubmed-3832171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-38321712013-11-18 Umbelliprenin Induces Apoptosis in CLL Cell Lines Ziai, Seyed Ali Gholami, Omid Iranshahi, Mehrdad Zamani, Amir Hassan Jeddi-Tehrani, Mahmood Iran J Pharm Res Original Article Chronic lymphocytic leukemia (CLL) remains an incurable disease that requires innovative new approaches to improve therapeutic outcome. Many Ferula species, including F. asa-foetida, synthesize terpenyloxy coumarins. One of these coumarins is umbelliprenin, which has been implicated with induction of apoptosis in some cancer cell lines. In this study induction of apoptosis by umbelliprenin on Jurkat T-CLL and Raji B-CLL cell lines was studied. In this regard, cells were incubated with various concentrations of umbelliprenin in-vitro for different times and assayed for apoptosis with annexin V–FITC/PI double staining flowcytometry method. Results showed that umbelliprenin induced apoptosis in leukemic cells in a dose- and time-dependent manner and that CLL cells were more susceptible to umbelliprenin induced cell death than normal peripheral blood mononuclear cell (PBMCs). Moreover, we study the induction of apoptosis in Jurkat cells by umbelliprenin in the presence of interleukin 4 (IL-4) as an agent that causes resistance to apoptosis in CLL cells, was also student. We showed that IL-4 can not reduce apoptotic effect of umbelliprenin. The preferential toxicity of umbelliprenin for CLL cells, supports the hypothesis that oral administration of umbelliprenin in the form of foods or folk medicines containing this coumarin, might enhance protection against the development of CLL in man with little side effects. In conclusion, umbelliprenin may be an effective therapeutic agent in the treatment of CLL, and thus clinical studies with umbelliprenin may be appropriate. Shaheed Beheshti University of Medical Sciences 2012 /pmc/articles/PMC3832171/ /pubmed/24250490 Text en © 2012 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ziai, Seyed Ali Gholami, Omid Iranshahi, Mehrdad Zamani, Amir Hassan Jeddi-Tehrani, Mahmood Umbelliprenin Induces Apoptosis in CLL Cell Lines |
title | Umbelliprenin Induces Apoptosis in CLL Cell Lines |
title_full | Umbelliprenin Induces Apoptosis in CLL Cell Lines |
title_fullStr | Umbelliprenin Induces Apoptosis in CLL Cell Lines |
title_full_unstemmed | Umbelliprenin Induces Apoptosis in CLL Cell Lines |
title_short | Umbelliprenin Induces Apoptosis in CLL Cell Lines |
title_sort | umbelliprenin induces apoptosis in cll cell lines |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832171/ https://www.ncbi.nlm.nih.gov/pubmed/24250490 |
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