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Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion

Plakoglobin (γ-catenin) is a homolog of β-catenin with dual adhesive and signaling functions. Plakoglobin participates in cell-cell adhesion as a component of the adherens junction and desmosomes whereas its signaling function is mediated by its interactions with various intracellular protein partne...

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Autores principales: Aktary, Zackie, Pasdar, Manijeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832639/
https://www.ncbi.nlm.nih.gov/pubmed/24260116
http://dx.doi.org/10.1371/journal.pone.0078388
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author Aktary, Zackie
Pasdar, Manijeh
author_facet Aktary, Zackie
Pasdar, Manijeh
author_sort Aktary, Zackie
collection PubMed
description Plakoglobin (γ-catenin) is a homolog of β-catenin with dual adhesive and signaling functions. Plakoglobin participates in cell-cell adhesion as a component of the adherens junction and desmosomes whereas its signaling function is mediated by its interactions with various intracellular protein partners. To determine the role of plakoglobin during tumorigenesis and metastasis, we expressed plakoglobin in the human tongue squamous cell carcinoma (SCC9) cells and compared the mRNA profiles of parental SCC9 cells and their plakoglobin-expressing transfectants (SCC9-PG). We observed that the mRNA levels of SATB1, the oncogenic chromatin remodeling factor, were decreased approximately 3-fold in SCC9-PG cells compared to parental SCC9 cells. Here, we showed that plakoglobin decreased levels of SATB1 mRNA and protein in SCC9-PG cells and that plakoglobin and p53 associated with the SATB1 promoter. Plakoglobin expression also resulted in decreased SATB1 promoter activity. These results were confirmed following plakoglobin expression in the very low plakoglobin expressing and invasive mammary carcinoma cell line MDA-MB-231 cells (MDA-231-PG). In addition, knockdown of endogenous plakoglobin in the non-invasive mammary carcinoma MCF-7 cells (MCF-7-shPG) resulted in increased SATB1 mRNA and protein. Plakoglobin expression also resulted in increased mRNA and protein levels of the metastasis suppressor Nm23-H1, a SATB1 target gene. Furthermore, the levels of various SATB1 target genes involved in tumorigenesis and metastasis were altered in MCF-7-shPG cells relative to parental MCF-7 cells. Finally, plakoglobin expression resulted in decreased in vitro proliferation, migration and invasion in different carcinoma cell lines. Together with the results of our previous studies, the data suggests that plakoglobin suppresses tumorigenesis and metastasis through the regulation of genes involved in these processes.
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spelling pubmed-38326392013-11-20 Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion Aktary, Zackie Pasdar, Manijeh PLoS One Research Article Plakoglobin (γ-catenin) is a homolog of β-catenin with dual adhesive and signaling functions. Plakoglobin participates in cell-cell adhesion as a component of the adherens junction and desmosomes whereas its signaling function is mediated by its interactions with various intracellular protein partners. To determine the role of plakoglobin during tumorigenesis and metastasis, we expressed plakoglobin in the human tongue squamous cell carcinoma (SCC9) cells and compared the mRNA profiles of parental SCC9 cells and their plakoglobin-expressing transfectants (SCC9-PG). We observed that the mRNA levels of SATB1, the oncogenic chromatin remodeling factor, were decreased approximately 3-fold in SCC9-PG cells compared to parental SCC9 cells. Here, we showed that plakoglobin decreased levels of SATB1 mRNA and protein in SCC9-PG cells and that plakoglobin and p53 associated with the SATB1 promoter. Plakoglobin expression also resulted in decreased SATB1 promoter activity. These results were confirmed following plakoglobin expression in the very low plakoglobin expressing and invasive mammary carcinoma cell line MDA-MB-231 cells (MDA-231-PG). In addition, knockdown of endogenous plakoglobin in the non-invasive mammary carcinoma MCF-7 cells (MCF-7-shPG) resulted in increased SATB1 mRNA and protein. Plakoglobin expression also resulted in increased mRNA and protein levels of the metastasis suppressor Nm23-H1, a SATB1 target gene. Furthermore, the levels of various SATB1 target genes involved in tumorigenesis and metastasis were altered in MCF-7-shPG cells relative to parental MCF-7 cells. Finally, plakoglobin expression resulted in decreased in vitro proliferation, migration and invasion in different carcinoma cell lines. Together with the results of our previous studies, the data suggests that plakoglobin suppresses tumorigenesis and metastasis through the regulation of genes involved in these processes. Public Library of Science 2013-11-08 /pmc/articles/PMC3832639/ /pubmed/24260116 http://dx.doi.org/10.1371/journal.pone.0078388 Text en © 2013 Aktary, Pasdar http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Aktary, Zackie
Pasdar, Manijeh
Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion
title Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion
title_full Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion
title_fullStr Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion
title_full_unstemmed Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion
title_short Plakoglobin Represses SATB1 Expression and Decreases In Vitro Proliferation, Migration and Invasion
title_sort plakoglobin represses satb1 expression and decreases in vitro proliferation, migration and invasion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832639/
https://www.ncbi.nlm.nih.gov/pubmed/24260116
http://dx.doi.org/10.1371/journal.pone.0078388
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