Cargando…
Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin
INTRODUCTION: Pulmonary fibrosis occurs due to fibroblast proliferation and collagen production in the lung and begins with alveolar inflammatory edema. Aquaporins (AQPs) play pivotal roles in lung fluid transport. In this study we establish the experimental model for pulmonary fibrosis in C57BL/6 m...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832812/ https://www.ncbi.nlm.nih.gov/pubmed/24273579 http://dx.doi.org/10.5114/aoms.2012.31011 |
_version_ | 1782291741563420672 |
---|---|
author | Gao, Xiwen Wang, Guifang Zhang, Wei Peng, Qing Xue, Min Jinhong, Hu |
author_facet | Gao, Xiwen Wang, Guifang Zhang, Wei Peng, Qing Xue, Min Jinhong, Hu |
author_sort | Gao, Xiwen |
collection | PubMed |
description | INTRODUCTION: Pulmonary fibrosis occurs due to fibroblast proliferation and collagen production in the lung and begins with alveolar inflammatory edema. Aquaporins (AQPs) play pivotal roles in lung fluid transport. In this study we establish the experimental model for pulmonary fibrosis in C57BL/6 mice to investigate expressions of AQP1 and AQP5 in lung tissue. MATERIAL AND METHODS: Mice in model groups were treated intratracheally with bleomycin with the dose of 5 mg/kg body weight. The mice were sacrificed at 1 week, 2 weeks and 3 weeks respectively. The left upper lungs were harvested for histopathologic H-E and Masson's staining. The mRNAs of AQP1 and AQP5 were analyzed by real-time polymerase chain reaction (real-time PCR) and the proteins of AQP1 and AQP5 were analyzed by western blotting. RESULTS: Real-time PCR showed that AQP1 mRNA in bleomycin 1 w, 2 w, and 3 w groups increased by 377%, 880% and 823% respectively compared to that in the control group (p < 0.01). Western blotting showed that the expression of AQP1 protein in bleomycin 1 w, 2 w, and 3 w groups increased by 53%, 144%, and 141%, respectively (p < 0.05). AQP5 mRNA in bleomycin 1 w and 2 w group decreased by 78% and 66%, respectively (p < 0.05). In bleomycin 2 w and 3 w groups it decreased by 69% and 80% (p < 0.05). CONCLUSIONS: The expression of AQP1 dramatically increased in pulmonary fibrosis. AQP1 plays an important role in the progress of pulmonary fibrosis. |
format | Online Article Text |
id | pubmed-3832812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-38328122013-11-22 Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin Gao, Xiwen Wang, Guifang Zhang, Wei Peng, Qing Xue, Min Jinhong, Hu Arch Med Sci Experimental Research INTRODUCTION: Pulmonary fibrosis occurs due to fibroblast proliferation and collagen production in the lung and begins with alveolar inflammatory edema. Aquaporins (AQPs) play pivotal roles in lung fluid transport. In this study we establish the experimental model for pulmonary fibrosis in C57BL/6 mice to investigate expressions of AQP1 and AQP5 in lung tissue. MATERIAL AND METHODS: Mice in model groups were treated intratracheally with bleomycin with the dose of 5 mg/kg body weight. The mice were sacrificed at 1 week, 2 weeks and 3 weeks respectively. The left upper lungs were harvested for histopathologic H-E and Masson's staining. The mRNAs of AQP1 and AQP5 were analyzed by real-time polymerase chain reaction (real-time PCR) and the proteins of AQP1 and AQP5 were analyzed by western blotting. RESULTS: Real-time PCR showed that AQP1 mRNA in bleomycin 1 w, 2 w, and 3 w groups increased by 377%, 880% and 823% respectively compared to that in the control group (p < 0.01). Western blotting showed that the expression of AQP1 protein in bleomycin 1 w, 2 w, and 3 w groups increased by 53%, 144%, and 141%, respectively (p < 0.05). AQP5 mRNA in bleomycin 1 w and 2 w group decreased by 78% and 66%, respectively (p < 0.05). In bleomycin 2 w and 3 w groups it decreased by 69% and 80% (p < 0.05). CONCLUSIONS: The expression of AQP1 dramatically increased in pulmonary fibrosis. AQP1 plays an important role in the progress of pulmonary fibrosis. Termedia Publishing House 2012-10-16 2013-10-31 /pmc/articles/PMC3832812/ /pubmed/24273579 http://dx.doi.org/10.5114/aoms.2012.31011 Text en Copyright © 2013 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Experimental Research Gao, Xiwen Wang, Guifang Zhang, Wei Peng, Qing Xue, Min Jinhong, Hu Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
title | Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
title_full | Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
title_fullStr | Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
title_full_unstemmed | Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
title_short | Expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
title_sort | expression of pulmonary aquaporin 1 is dramatically upregulated in mice with pulmonary fibrosis induced by bleomycin |
topic | Experimental Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832812/ https://www.ncbi.nlm.nih.gov/pubmed/24273579 http://dx.doi.org/10.5114/aoms.2012.31011 |
work_keys_str_mv | AT gaoxiwen expressionofpulmonaryaquaporin1isdramaticallyupregulatedinmicewithpulmonaryfibrosisinducedbybleomycin AT wangguifang expressionofpulmonaryaquaporin1isdramaticallyupregulatedinmicewithpulmonaryfibrosisinducedbybleomycin AT zhangwei expressionofpulmonaryaquaporin1isdramaticallyupregulatedinmicewithpulmonaryfibrosisinducedbybleomycin AT pengqing expressionofpulmonaryaquaporin1isdramaticallyupregulatedinmicewithpulmonaryfibrosisinducedbybleomycin AT xuemin expressionofpulmonaryaquaporin1isdramaticallyupregulatedinmicewithpulmonaryfibrosisinducedbybleomycin AT jinhonghu expressionofpulmonaryaquaporin1isdramaticallyupregulatedinmicewithpulmonaryfibrosisinducedbybleomycin |